Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox
The 3561 m Vostok ice core sample originating from the subglacial Lake Vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium Hydrogenophilus thermoluteolus reported earlier in the 3607 m accretio...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2006
|
Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-00135745 https://doi.org/10.1111/j.1462-2920.2006.01087.x |
id |
ftccsdartic:oai:HAL:hal-00135745v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Lavire, Céline Normand, P. Alekhina, Irina Bulat, Serguey Prieur, Daniel Birrien, Jean-Louis Fournier, Pascale Hänni, Catherine Petit, Jean-Robert Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
topic_facet |
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
The 3561 m Vostok ice core sample originating from the subglacial Lake Vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium Hydrogenophilus thermoluteolus reported earlier in the 3607 m accretion ice sample. PCR and molecular-phylogenetic analyses performed in two independent laboratories were made using different 16S rRNA gene ( rrs ) targeted primers. As a result, rrs -targeted PCR permitted to recover several very closely related clones with a small genetic distance to Hydrogenophilus thermoluteolus ( < 1%). In addition, RubisCO ( cbbL or rbcL ) and NiFe-Hydrogenase (hoxV or hupL ) targeted PCR have also allowed to recover sequences highly related to Hydrogenophilus thermoluteolus . All these results point to the presence of thermophilic chemoautotrophic microorganisms in Lake Vostok accretion ice. They presumably originate from deep faults in the bedrock cavity containing the lake in which episodes of seismotectonic activity would release debris along with microbial cells. |
author2 |
Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) Saint Petersburg Nuclear Physics Institute RAS St Petersburg Nuclear Physics Institute Laboratoire de glaciologie et géophysique de l'environnement (LGGE) Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Grenoble (OSUG) Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) Laboratoire de microbiologie des environnements extrêmophiles (LM2E) Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC) Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon |
format |
Article in Journal/Newspaper |
author |
Lavire, Céline Normand, P. Alekhina, Irina Bulat, Serguey Prieur, Daniel Birrien, Jean-Louis Fournier, Pascale Hänni, Catherine Petit, Jean-Robert |
author_facet |
Lavire, Céline Normand, P. Alekhina, Irina Bulat, Serguey Prieur, Daniel Birrien, Jean-Louis Fournier, Pascale Hänni, Catherine Petit, Jean-Robert |
author_sort |
Lavire, Céline |
title |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_short |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_full |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_fullStr |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_full_unstemmed |
Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox |
title_sort |
presence of hydrogenophilus thermoluteolus dna in accretion ice in the subglacial lake vostok, antarctica, assessed using rrs, cbb and hox |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.archives-ouvertes.fr/hal-00135745 https://doi.org/10.1111/j.1462-2920.2006.01087.x |
long_lat |
ENVELOPE(76.108,76.108,-69.415,-69.415) ENVELOPE(106.000,106.000,-77.500,-77.500) |
geographic |
Frozen Lake Lake Vostok |
geographic_facet |
Frozen Lake Lake Vostok |
genre |
Antarc* Antarctica ice core |
genre_facet |
Antarc* Antarctica ice core |
op_source |
ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.archives-ouvertes.fr/hal-00135745 Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2006, 8 (12), pp.2106-2114. ⟨10.1111/j.1462-2920.2006.01087.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1462-2920.2006.01087.x hal-00135745 https://hal.archives-ouvertes.fr/hal-00135745 doi:10.1111/j.1462-2920.2006.01087.x PRODINRA: 251868 |
op_doi |
https://doi.org/10.1111/j.1462-2920.2006.01087.x |
container_title |
Environmental Microbiology |
container_volume |
8 |
container_issue |
12 |
container_start_page |
2106 |
op_container_end_page |
2114 |
_version_ |
1766191360669384704 |
spelling |
ftccsdartic:oai:HAL:hal-00135745v1 2023-05-15T13:43:38+02:00 Presence of Hydrogenophilus thermoluteolus DNA in accretion ice in the subglacial Lake Vostok, Antarctica, assessed using rrs, cbb and hox Lavire, Céline Normand, P. Alekhina, Irina Bulat, Serguey Prieur, Daniel Birrien, Jean-Louis Fournier, Pascale Hänni, Catherine Petit, Jean-Robert Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) Saint Petersburg Nuclear Physics Institute RAS St Petersburg Nuclear Physics Institute Laboratoire de glaciologie et géophysique de l'environnement (LGGE) Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Grenoble (OSUG) Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) Laboratoire de microbiologie des environnements extrêmophiles (LM2E) Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC) Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon 2006 https://hal.archives-ouvertes.fr/hal-00135745 https://doi.org/10.1111/j.1462-2920.2006.01087.x en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1462-2920.2006.01087.x hal-00135745 https://hal.archives-ouvertes.fr/hal-00135745 doi:10.1111/j.1462-2920.2006.01087.x PRODINRA: 251868 ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.archives-ouvertes.fr/hal-00135745 Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2006, 8 (12), pp.2106-2114. ⟨10.1111/j.1462-2920.2006.01087.x⟩ [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2006 ftccsdartic https://doi.org/10.1111/j.1462-2920.2006.01087.x 2021-10-24T22:31:30Z The 3561 m Vostok ice core sample originating from the subglacial Lake Vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium Hydrogenophilus thermoluteolus reported earlier in the 3607 m accretion ice sample. PCR and molecular-phylogenetic analyses performed in two independent laboratories were made using different 16S rRNA gene ( rrs ) targeted primers. As a result, rrs -targeted PCR permitted to recover several very closely related clones with a small genetic distance to Hydrogenophilus thermoluteolus ( < 1%). In addition, RubisCO ( cbbL or rbcL ) and NiFe-Hydrogenase (hoxV or hupL ) targeted PCR have also allowed to recover sequences highly related to Hydrogenophilus thermoluteolus . All these results point to the presence of thermophilic chemoautotrophic microorganisms in Lake Vostok accretion ice. They presumably originate from deep faults in the bedrock cavity containing the lake in which episodes of seismotectonic activity would release debris along with microbial cells. Article in Journal/Newspaper Antarc* Antarctica ice core Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Frozen Lake ENVELOPE(76.108,76.108,-69.415,-69.415) Lake Vostok ENVELOPE(106.000,106.000,-77.500,-77.500) Environmental Microbiology 8 12 2106 2114 |