Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia
International audience Background Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H....
Published in: | Parasites & Vectors |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2023
|
Subjects: | |
Online Access: | https://anses.hal.science/anses-04228183 https://anses.hal.science/anses-04228183/document https://anses.hal.science/anses-04228183/file/s13071-023-05879-x.pdf https://doi.org/10.1186/s13071-023-05879-x |
id |
ftccsdartic:oai:HAL:anses-04228183v1 |
---|---|
record_format |
openpolar |
spelling |
ftccsdartic:oai:HAL:anses-04228183v1 2023-12-31T10:09:15+01:00 Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia Miljević, Milan Rajičić, Marija Umhang, Gérald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena University of Belgrade Belgrade Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Faculty of Sciences Novi Sad University of Novi Sad Ministry of Education, Science and Technological Development of the Republic of Serbia 2023-07-25 https://anses.hal.science/anses-04228183 https://anses.hal.science/anses-04228183/document https://anses.hal.science/anses-04228183/file/s13071-023-05879-x.pdf https://doi.org/10.1186/s13071-023-05879-x en eng HAL CCSD BioMed Central info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-023-05879-x info:eu-repo/semantics/altIdentifier/pmid/37491284 anses-04228183 https://anses.hal.science/anses-04228183 https://anses.hal.science/anses-04228183/document https://anses.hal.science/anses-04228183/file/s13071-023-05879-x.pdf doi:10.1186/s13071-023-05879-x PUBMED: 37491284 PUBMEDCENTRAL: PMC10369706 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1756-3305 EISSN: 1756-3305 Parasites & Vectors https://anses.hal.science/anses-04228183 Parasites & Vectors, 2023, 16 (1), pp.250. ⟨10.1186/s13071-023-05879-x⟩ https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-023-05879-x Parasite Taenia Rodents cox1 12S rDNA Phylogenetics [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology info:eu-repo/semantics/article Journal articles 2023 ftccsdartic https://doi.org/10.1186/s13071-023-05879-x 2023-12-02T23:39:28Z International audience Background Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu lato (s.l.) species is difficult without the use of molecular methods. The aim of this study was to identify and explore the genetic diversity of Hydatigera and other taeniid species. Methods Ten different small mammals species (856 individuals) (Rattus rattus, three Apodemus, three Arvicolinae and three Soricidae species) were examined from 2013 to 2023. Captured animals were visually examined for cysts and visible lesions. Two markers were used for amplification and sequencing: cox1 and 12S rDNA. Results Molecular analysis of cysts and visible lesions revealed four taeniid species: Hydatigera kamiyai, H. taeniaeformis sensu stricto (s.s.), Taenia martis and T. crassiceps. Hydatigera kamiyai was found in Apodemus flavicollis, A. agrarius, Microtus arvalis and Crocidrua leucodon, while H. taeniaeformis s.s. is registered in R. rattus. Hydatigera kamiyai cox1 sequences clustered with European populations and showed at least 25 nucleotid differences compared to Asian, African, Australian and one of our isolates of H. taeniaeformis s.s acquired from a rat, followed by large sequence distances (9.4% to 12.9%), indicating clear molecular distinction of two species. Conclusions This is one of the few mitochondrial gene-based studies performed after the description of cryptic entities within the Hydatigera taeniaeformis s.l. complex and represents a valuable contribution to understanding of genetic diversity, host suitability and geographic distribution of these tapeworm species. Also, our study provides an important basis of molecular data from this part of Europe for further studies. We emphasize the importance of additional studies of intermediate hosts, especially rats from Europe and Apodemus spp. ... Article in Journal/Newspaper Microtus arvalis Rattus rattus Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Parasites & Vectors 16 1 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Parasite Taenia Rodents cox1 12S rDNA Phylogenetics [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology |
spellingShingle |
Parasite Taenia Rodents cox1 12S rDNA Phylogenetics [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Miljević, Milan Rajičić, Marija Umhang, Gérald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
topic_facet |
Parasite Taenia Rodents cox1 12S rDNA Phylogenetics [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology |
description |
International audience Background Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu lato (s.l.) species is difficult without the use of molecular methods. The aim of this study was to identify and explore the genetic diversity of Hydatigera and other taeniid species. Methods Ten different small mammals species (856 individuals) (Rattus rattus, three Apodemus, three Arvicolinae and three Soricidae species) were examined from 2013 to 2023. Captured animals were visually examined for cysts and visible lesions. Two markers were used for amplification and sequencing: cox1 and 12S rDNA. Results Molecular analysis of cysts and visible lesions revealed four taeniid species: Hydatigera kamiyai, H. taeniaeformis sensu stricto (s.s.), Taenia martis and T. crassiceps. Hydatigera kamiyai was found in Apodemus flavicollis, A. agrarius, Microtus arvalis and Crocidrua leucodon, while H. taeniaeformis s.s. is registered in R. rattus. Hydatigera kamiyai cox1 sequences clustered with European populations and showed at least 25 nucleotid differences compared to Asian, African, Australian and one of our isolates of H. taeniaeformis s.s acquired from a rat, followed by large sequence distances (9.4% to 12.9%), indicating clear molecular distinction of two species. Conclusions This is one of the few mitochondrial gene-based studies performed after the description of cryptic entities within the Hydatigera taeniaeformis s.l. complex and represents a valuable contribution to understanding of genetic diversity, host suitability and geographic distribution of these tapeworm species. Also, our study provides an important basis of molecular data from this part of Europe for further studies. We emphasize the importance of additional studies of intermediate hosts, especially rats from Europe and Apodemus spp. ... |
author2 |
University of Belgrade Belgrade Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Faculty of Sciences Novi Sad University of Novi Sad Ministry of Education, Science and Technological Development of the Republic of Serbia |
format |
Article in Journal/Newspaper |
author |
Miljević, Milan Rajičić, Marija Umhang, Gérald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena |
author_facet |
Miljević, Milan Rajičić, Marija Umhang, Gérald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena |
author_sort |
Miljević, Milan |
title |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_short |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_full |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_fullStr |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_full_unstemmed |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_sort |
cryptic species hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in serbia |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://anses.hal.science/anses-04228183 https://anses.hal.science/anses-04228183/document https://anses.hal.science/anses-04228183/file/s13071-023-05879-x.pdf https://doi.org/10.1186/s13071-023-05879-x |
genre |
Microtus arvalis Rattus rattus |
genre_facet |
Microtus arvalis Rattus rattus |
op_source |
ISSN: 1756-3305 EISSN: 1756-3305 Parasites & Vectors https://anses.hal.science/anses-04228183 Parasites & Vectors, 2023, 16 (1), pp.250. ⟨10.1186/s13071-023-05879-x⟩ https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-023-05879-x |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-023-05879-x info:eu-repo/semantics/altIdentifier/pmid/37491284 anses-04228183 https://anses.hal.science/anses-04228183 https://anses.hal.science/anses-04228183/document https://anses.hal.science/anses-04228183/file/s13071-023-05879-x.pdf doi:10.1186/s13071-023-05879-x PUBMED: 37491284 PUBMEDCENTRAL: PMC10369706 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s13071-023-05879-x |
container_title |
Parasites & Vectors |
container_volume |
16 |
container_issue |
1 |
_version_ |
1786842300669755392 |