Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity
International audience To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolop...
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ftccsdartic:oai:HAL:anses-03294542v1 2023-05-15T17:13:46+02:00 Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity Šimić, Ivana Zorec, Tomaž Mark Lojkić, Ivana Krešić, Nina Poljak, Mario Cliquet, Florence Picard-Meyer, Evelyne Wasniewski, Marine Zrnčić, Vida Ćukušić, Anđela Bedeković, Tomislav Croatian Veterinary Institute University of Ljubljana Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Croatian Biospeleological Society 2020 https://hal-anses.archives-ouvertes.fr/anses-03294542 https://hal-anses.archives-ouvertes.fr/anses-03294542/document https://hal-anses.archives-ouvertes.fr/anses-03294542/file/viruses-12-00891-v2.pdf https://doi.org/10.3390/v12080891 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/v12080891 anses-03294542 https://hal-anses.archives-ouvertes.fr/anses-03294542 https://hal-anses.archives-ouvertes.fr/anses-03294542/document https://hal-anses.archives-ouvertes.fr/anses-03294542/file/viruses-12-00891-v2.pdf doi:10.3390/v12080891 info:eu-repo/semantics/OpenAccess ISSN: 1999-4915 Viruses https://hal-anses.archives-ouvertes.fr/anses-03294542 Viruses, MDPI, 2020, 12 (8), pp.891. ⟨10.3390/v12080891⟩ Bats Viral metagenomics Croatia Virus Diversity [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie info:eu-repo/semantics/article Journal articles 2020 ftccsdartic https://doi.org/10.3390/v12080891 2021-11-06T23:35:00Z International audience To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus sequences were assigned to retroviruses, parvoviruses, iridoviruses, and poxviruses. We further report the complete genome sequence of a novel adeno-associated virus, densovirus and a near complete length genome sequence of a novel iflavirus. Additionally, one of the most interesting findings in this study was the difference in viromes between two contrasting habitats, the continental and Mediterranean Croatia. Article in Journal/Newspaper Myotis nattereri Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) Guano ENVELOPE(141.604,141.604,-66.775,-66.775) Viruses 12 8 891 |
institution |
Open Polar |
collection |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
op_collection_id |
ftccsdartic |
language |
English |
topic |
Bats Viral metagenomics Croatia Virus Diversity [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie |
spellingShingle |
Bats Viral metagenomics Croatia Virus Diversity [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Šimić, Ivana Zorec, Tomaž Mark Lojkić, Ivana Krešić, Nina Poljak, Mario Cliquet, Florence Picard-Meyer, Evelyne Wasniewski, Marine Zrnčić, Vida Ćukušić, Anđela Bedeković, Tomislav Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
topic_facet |
Bats Viral metagenomics Croatia Virus Diversity [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie |
description |
International audience To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus sequences were assigned to retroviruses, parvoviruses, iridoviruses, and poxviruses. We further report the complete genome sequence of a novel adeno-associated virus, densovirus and a near complete length genome sequence of a novel iflavirus. Additionally, one of the most interesting findings in this study was the difference in viromes between two contrasting habitats, the continental and Mediterranean Croatia. |
author2 |
Croatian Veterinary Institute University of Ljubljana Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Croatian Biospeleological Society |
format |
Article in Journal/Newspaper |
author |
Šimić, Ivana Zorec, Tomaž Mark Lojkić, Ivana Krešić, Nina Poljak, Mario Cliquet, Florence Picard-Meyer, Evelyne Wasniewski, Marine Zrnčić, Vida Ćukušić, Anđela Bedeković, Tomislav |
author_facet |
Šimić, Ivana Zorec, Tomaž Mark Lojkić, Ivana Krešić, Nina Poljak, Mario Cliquet, Florence Picard-Meyer, Evelyne Wasniewski, Marine Zrnčić, Vida Ćukušić, Anđela Bedeković, Tomislav |
author_sort |
Šimić, Ivana |
title |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_short |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_full |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_fullStr |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_full_unstemmed |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_sort |
viral metagenomic profiling of croatian bat population reveals sample and habitat dependent diversity |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal-anses.archives-ouvertes.fr/anses-03294542 https://hal-anses.archives-ouvertes.fr/anses-03294542/document https://hal-anses.archives-ouvertes.fr/anses-03294542/file/viruses-12-00891-v2.pdf https://doi.org/10.3390/v12080891 |
long_lat |
ENVELOPE(141.604,141.604,-66.775,-66.775) |
geographic |
Guano |
geographic_facet |
Guano |
genre |
Myotis nattereri |
genre_facet |
Myotis nattereri |
op_source |
ISSN: 1999-4915 Viruses https://hal-anses.archives-ouvertes.fr/anses-03294542 Viruses, MDPI, 2020, 12 (8), pp.891. ⟨10.3390/v12080891⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/v12080891 anses-03294542 https://hal-anses.archives-ouvertes.fr/anses-03294542 https://hal-anses.archives-ouvertes.fr/anses-03294542/document https://hal-anses.archives-ouvertes.fr/anses-03294542/file/viruses-12-00891-v2.pdf doi:10.3390/v12080891 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/v12080891 |
container_title |
Viruses |
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12 |
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8 |
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891 |
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