Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity

• Published in: Viruses
• Main Authors: Šimić, Ivana, Zorec, Tomaž Mark, Lojkić, Ivana, Krešić, Nina, Poljak, Mario, Cliquet, Florence, Picard-Meyer, Evelyne, Wasniewski, Marine, Zrnčić, Vida, Ćukušić, Anđela, Bedeković, Tomislav
• Other Authors: Croatian Veterinary Institute, University of Ljubljana, Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Croatian Biospeleological Society
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: Bats; Viral metagenomics; Croatia; Virus; Diversity; [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology; [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie; Guano; Myotis nattereri
• Online Access: https://hal-anses.archives-ouvertes.fr/anses-03294542; https://hal-anses.archives-ouvertes.fr/anses-03294542/document; https://hal-anses.archives-ouvertes.fr/anses-03294542/file/viruses-12-00891-v2.pdf; https://doi.org/10.3390/v12080891

International audience To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus sequences were assigned to retroviruses, parvoviruses, iridoviruses, and poxviruses. We further report the complete genome sequence of a novel adeno-associated virus, densovirus and a near complete length genome sequence of a novel iflavirus. Additionally, one of the most interesting findings in this study was the difference in viromes between two contrasting habitats, the continental and Mediterranean Croatia.