Towards quantitative microbiome community profiling using internal standards

• Main Authors: Lin, Y., Gifford, S., Ducklow, H., Schofield, O., Cassara, N.
• Other Authors: College of Arts and Sciences, Department of Marine Sciences
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 2018
• Subjects: Community profiling; Internal standard; Amplicon sequencing; Marine microbiome; Antarc*; Antarctica
• Online Access: https://doi.org/10.17615/hhfw-ey69; https://cdr.lib.unc.edu/downloads/8623j7243?file=thumbnail; https://cdr.lib.unc.edu/downloads/8623j7243

An inherent issue in high-throughput rRNA gene tag sequencing microbiome surveys is that they provide compositional data in relative abundances. This often leads to spurious correlations, making the interpretation of relationships to biogeochemical rates challenging. To overcome this issue, we quantitatively estimated the abundance of microorganisms by spiking in known amounts of internal DNA standards. Using a 3-year sample set of diverse microbial communities from the Western Antarctica Peninsula, we demonstrated that the internal standard method yielded community profiles and taxon cooccurrence patterns substantially different from those derived using relative abundances. We found that the method provided results consistent with the traditional CHEMTAX analysis of pigments and total bacterial counts by flow cytometry. Using the internal standard method, we also showed that chloroplast 16S rRNA gene data in microbial surveys can be used to estimate abundances of certain eukaryotic phototrophs such as cryptophytes and diatoms. In Phaeocystis, scatter in the 16S/18S rRNA gene ratio may be explained by physiological adaptation to environmental conditions. We conclude that the internal standard method, when applied to rRNA gene microbial community profiling, is quantitative and that its application will substantially improve our understanding of microbial ecosystems.