Zonation of the active methane-cycling community in deep subsurface sediments of the Peru trench
The production and anaerobic oxidation of methane (AOM) by microorganisms is widespread in organic-rich deep subseafloor sediments. Yet, the organisms that carry out these processes remain largely unknown. Here we identify members of the methane-cycling microbial community in deep subsurface, hydrat...
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ftcarolinadr:cdr.lib.unc.edu:d791ss48v 2023-11-05T03:43:26+01:00 Zonation of the active methane-cycling community in deep subsurface sediments of the Peru trench Lever, Mark A. Alperin, Marc J. Hinrichs, Kai-Uwe Teske, Andreas 2023 https://doi.org/10.17615/dq34-4638 https://cdr.lib.unc.edu/downloads/2v23w516t?file=thumbnail https://cdr.lib.unc.edu/downloads/2v23w516t English eng Frontiers Media https://doi.org/10.17615/dq34-4638 https://cdr.lib.unc.edu/downloads/2v23w516t?file=thumbnail https://cdr.lib.unc.edu/downloads/2v23w516t http://rightsstatements.org/vocab/InC/1.0/ Frontiers in Microbiology, 14 anaerobic oxidation of methane methanogenesis carbon isotopes deep biosphere mcrA methane hydrate subseafloor sediment ocean drilling Article 2023 ftcarolinadr https://doi.org/10.17615/dq34-4638 2023-10-07T22:30:05Z The production and anaerobic oxidation of methane (AOM) by microorganisms is widespread in organic-rich deep subseafloor sediments. Yet, the organisms that carry out these processes remain largely unknown. Here we identify members of the methane-cycling microbial community in deep subsurface, hydrate-containing sediments of the Peru Trench by targeting functional genes of the alpha subunit of methyl coenzyme M reductase (mcrA). The mcrA profile reveals a distinct community zonation that partially matches the zonation of methane oxidizing and –producing activity inferred from sulfate and methane concentrations and carbon-isotopic compositions of methane and dissolved inorganic carbon (DIC). McrA appears absent from sulfate-rich sediments that are devoid of methane, but mcrA sequences belonging to putatively methane-oxidizing ANME-1a-b occur from the zone of methane oxidation to several meters into the methanogenesis zone. A sister group of ANME-1a-b, referred to as ANME-1d, and members of putatively aceticlastic Methanothrix (formerly Methanosaeta) occur throughout the remaining methanogenesis zone. Analyses of 16S rRNA and mcrA-mRNA indicate that the methane-cycling community is alive throughout (rRNA to 230 mbsf) and active in at least parts of the sediment column (mRNA at 44 mbsf). Carbon-isotopic depletions of methane relative to DIC (−80 to −86‰) suggest mostly methane production by CO2 reduction and thus seem at odds with the widespread detection of ANME-1 and Methanothrix. We explain this apparent contradiction based on recent insights into the metabolisms of both ANME-1 and Methanothricaceae, which indicate the potential for methanogenetic growth by CO2 reduction in both groups. Article in Journal/Newspaper Methane hydrate Carolina Digital Repository (UNC - University of North Carolina) |
institution |
Open Polar |
collection |
Carolina Digital Repository (UNC - University of North Carolina) |
op_collection_id |
ftcarolinadr |
language |
English |
topic |
anaerobic oxidation of methane methanogenesis carbon isotopes deep biosphere mcrA methane hydrate subseafloor sediment ocean drilling |
spellingShingle |
anaerobic oxidation of methane methanogenesis carbon isotopes deep biosphere mcrA methane hydrate subseafloor sediment ocean drilling Lever, Mark A. Alperin, Marc J. Hinrichs, Kai-Uwe Teske, Andreas Zonation of the active methane-cycling community in deep subsurface sediments of the Peru trench |
topic_facet |
anaerobic oxidation of methane methanogenesis carbon isotopes deep biosphere mcrA methane hydrate subseafloor sediment ocean drilling |
description |
The production and anaerobic oxidation of methane (AOM) by microorganisms is widespread in organic-rich deep subseafloor sediments. Yet, the organisms that carry out these processes remain largely unknown. Here we identify members of the methane-cycling microbial community in deep subsurface, hydrate-containing sediments of the Peru Trench by targeting functional genes of the alpha subunit of methyl coenzyme M reductase (mcrA). The mcrA profile reveals a distinct community zonation that partially matches the zonation of methane oxidizing and –producing activity inferred from sulfate and methane concentrations and carbon-isotopic compositions of methane and dissolved inorganic carbon (DIC). McrA appears absent from sulfate-rich sediments that are devoid of methane, but mcrA sequences belonging to putatively methane-oxidizing ANME-1a-b occur from the zone of methane oxidation to several meters into the methanogenesis zone. A sister group of ANME-1a-b, referred to as ANME-1d, and members of putatively aceticlastic Methanothrix (formerly Methanosaeta) occur throughout the remaining methanogenesis zone. Analyses of 16S rRNA and mcrA-mRNA indicate that the methane-cycling community is alive throughout (rRNA to 230 mbsf) and active in at least parts of the sediment column (mRNA at 44 mbsf). Carbon-isotopic depletions of methane relative to DIC (−80 to −86‰) suggest mostly methane production by CO2 reduction and thus seem at odds with the widespread detection of ANME-1 and Methanothrix. We explain this apparent contradiction based on recent insights into the metabolisms of both ANME-1 and Methanothricaceae, which indicate the potential for methanogenetic growth by CO2 reduction in both groups. |
format |
Article in Journal/Newspaper |
author |
Lever, Mark A. Alperin, Marc J. Hinrichs, Kai-Uwe Teske, Andreas |
author_facet |
Lever, Mark A. Alperin, Marc J. Hinrichs, Kai-Uwe Teske, Andreas |
author_sort |
Lever, Mark A. |
title |
Zonation of the active methane-cycling community in deep subsurface sediments of the Peru trench |
title_short |
Zonation of the active methane-cycling community in deep subsurface sediments of the Peru trench |
title_full |
Zonation of the active methane-cycling community in deep subsurface sediments of the Peru trench |
title_fullStr |
Zonation of the active methane-cycling community in deep subsurface sediments of the Peru trench |
title_full_unstemmed |
Zonation of the active methane-cycling community in deep subsurface sediments of the Peru trench |
title_sort |
zonation of the active methane-cycling community in deep subsurface sediments of the peru trench |
publisher |
Frontiers Media |
publishDate |
2023 |
url |
https://doi.org/10.17615/dq34-4638 https://cdr.lib.unc.edu/downloads/2v23w516t?file=thumbnail https://cdr.lib.unc.edu/downloads/2v23w516t |
genre |
Methane hydrate |
genre_facet |
Methane hydrate |
op_source |
Frontiers in Microbiology, 14 |
op_relation |
https://doi.org/10.17615/dq34-4638 https://cdr.lib.unc.edu/downloads/2v23w516t?file=thumbnail https://cdr.lib.unc.edu/downloads/2v23w516t |
op_rights |
http://rightsstatements.org/vocab/InC/1.0/ |
op_doi |
https://doi.org/10.17615/dq34-4638 |
_version_ |
1781701573579112448 |