Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice
Carbonic anhydrases are a family of enzymes that catalyze the reversible condensation of water and carbon dioxide to carbonic acid, which spontaneously dissociates to bicarbonate. Carbonic anhydrase III (Car3) is nutritionally regulated at both the mRNA and protein level. It is highly enriched in ti...
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ftcarolinadr:cdr.lib.unc.edu:9p290g33k 2023-12-03T10:21:02+01:00 Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice Renner, Sarah W. Walker, Lauren M. Forsberg, Lawrence J. Sexton, Jonathan Z. Brenman, Jay E. 2017 https://doi.org/10.17615/2hr0-2a05 https://cdr.lib.unc.edu/downloads/41687p48f?file=thumbnail https://cdr.lib.unc.edu/downloads/41687p48f English eng https://doi.org/10.17615/2hr0-2a05 https://cdr.lib.unc.edu/downloads/41687p48f?file=thumbnail https://cdr.lib.unc.edu/downloads/41687p48f http://rightsstatements.org/vocab/InC/1.0/ PloS One, 12(4) Body Composition Male Diet High-Fat Fatty Acids Mice Knockout Carbonic Anhydrase III Liver Animals Adipose Tissue White Body Weight Insulin Resistance Lipogenesis Brown Lipid Metabolism Article 2017 ftcarolinadr https://doi.org/10.17615/2hr0-2a05 2023-11-04T23:26:33Z Carbonic anhydrases are a family of enzymes that catalyze the reversible condensation of water and carbon dioxide to carbonic acid, which spontaneously dissociates to bicarbonate. Carbonic anhydrase III (Car3) is nutritionally regulated at both the mRNA and protein level. It is highly enriched in tissues that synthesize and/or store fat: liver, white adipose tissue, brown adipose tissue, and skeletal muscle. Previous characterization of Car3 knockout mice focused on mice fed standard diets, not high-fat diets that significantly alter the tissues that highly express Car3. We observed lower protein levels of Car3 in high-fat diet fed mice treated with niclosamide, a drug published to improve fatty liver symptoms in mice. However, it is unknown if Car3 is simply a biomarker reflecting lipid accumulation or whether it has a functional role in regulating lipid metabolism. We focused our in vitro studies toward metabolic pathways that require bicarbonate. To further determine the role of Car3 in metabolism, we measured de novo fatty acid synthesis with in vitro radiolabeled experiments and examined metabolic biomarkers in Car3 knockout and wild type mice fed high-fat diet. Specifically, we analyzed body weight, body composition, metabolic rate, insulin resistance, serum and tissue triglycerides. Our results indicate that Car3 is not required for de novo lipogenesis, and Car3 knockout mice fed high-fat diet do not have significant differences in responses to various diets to wild type mice. Article in Journal/Newspaper Carbonic acid Carolina Digital Repository (UNC - University of North Carolina) |
institution |
Open Polar |
collection |
Carolina Digital Repository (UNC - University of North Carolina) |
op_collection_id |
ftcarolinadr |
language |
English |
topic |
Body Composition Male Diet High-Fat Fatty Acids Mice Knockout Carbonic Anhydrase III Liver Animals Adipose Tissue White Body Weight Insulin Resistance Lipogenesis Brown Lipid Metabolism |
spellingShingle |
Body Composition Male Diet High-Fat Fatty Acids Mice Knockout Carbonic Anhydrase III Liver Animals Adipose Tissue White Body Weight Insulin Resistance Lipogenesis Brown Lipid Metabolism Renner, Sarah W. Walker, Lauren M. Forsberg, Lawrence J. Sexton, Jonathan Z. Brenman, Jay E. Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice |
topic_facet |
Body Composition Male Diet High-Fat Fatty Acids Mice Knockout Carbonic Anhydrase III Liver Animals Adipose Tissue White Body Weight Insulin Resistance Lipogenesis Brown Lipid Metabolism |
description |
Carbonic anhydrases are a family of enzymes that catalyze the reversible condensation of water and carbon dioxide to carbonic acid, which spontaneously dissociates to bicarbonate. Carbonic anhydrase III (Car3) is nutritionally regulated at both the mRNA and protein level. It is highly enriched in tissues that synthesize and/or store fat: liver, white adipose tissue, brown adipose tissue, and skeletal muscle. Previous characterization of Car3 knockout mice focused on mice fed standard diets, not high-fat diets that significantly alter the tissues that highly express Car3. We observed lower protein levels of Car3 in high-fat diet fed mice treated with niclosamide, a drug published to improve fatty liver symptoms in mice. However, it is unknown if Car3 is simply a biomarker reflecting lipid accumulation or whether it has a functional role in regulating lipid metabolism. We focused our in vitro studies toward metabolic pathways that require bicarbonate. To further determine the role of Car3 in metabolism, we measured de novo fatty acid synthesis with in vitro radiolabeled experiments and examined metabolic biomarkers in Car3 knockout and wild type mice fed high-fat diet. Specifically, we analyzed body weight, body composition, metabolic rate, insulin resistance, serum and tissue triglycerides. Our results indicate that Car3 is not required for de novo lipogenesis, and Car3 knockout mice fed high-fat diet do not have significant differences in responses to various diets to wild type mice. |
format |
Article in Journal/Newspaper |
author |
Renner, Sarah W. Walker, Lauren M. Forsberg, Lawrence J. Sexton, Jonathan Z. Brenman, Jay E. |
author_facet |
Renner, Sarah W. Walker, Lauren M. Forsberg, Lawrence J. Sexton, Jonathan Z. Brenman, Jay E. |
author_sort |
Renner, Sarah W. |
title |
Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice |
title_short |
Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice |
title_full |
Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice |
title_fullStr |
Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice |
title_full_unstemmed |
Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice |
title_sort |
carbonic anhydrase iii (car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice |
publishDate |
2017 |
url |
https://doi.org/10.17615/2hr0-2a05 https://cdr.lib.unc.edu/downloads/41687p48f?file=thumbnail https://cdr.lib.unc.edu/downloads/41687p48f |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
PloS One, 12(4) |
op_relation |
https://doi.org/10.17615/2hr0-2a05 https://cdr.lib.unc.edu/downloads/41687p48f?file=thumbnail https://cdr.lib.unc.edu/downloads/41687p48f |
op_rights |
http://rightsstatements.org/vocab/InC/1.0/ |
op_doi |
https://doi.org/10.17615/2hr0-2a05 |
_version_ |
1784268588459229184 |