Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard)

Measurements of the spectrum of extracellular enzymes present in an environment can indicate the nature of organic substrates available to microorganisms. We report the activities in an Arctic fjord (Smeerenburgfjord, Svalbard) of the extracellular carbohydrate hydrolases a-galactosidase, β-glucosid...

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Main Authors: Steen, Andrew D., Arnosti, Carol
Format: Article in Journal/Newspaper
Language:English
Published: 2013
Subjects:
Online Access:https://doi.org/10.17615/b8v7-7s82
https://cdr.lib.unc.edu/downloads/k3569d269?file=thumbnail
https://cdr.lib.unc.edu/downloads/k3569d269
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spelling ftcarolinadr:cdr.lib.unc.edu:3x816w48h 2023-11-12T04:11:44+01:00 Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard) Steen, Andrew D. Arnosti, Carol 2013 https://doi.org/10.17615/b8v7-7s82 https://cdr.lib.unc.edu/downloads/k3569d269?file=thumbnail https://cdr.lib.unc.edu/downloads/k3569d269 English eng https://doi.org/10.17615/b8v7-7s82 https://cdr.lib.unc.edu/downloads/k3569d269?file=thumbnail https://cdr.lib.unc.edu/downloads/k3569d269 http://rightsstatements.org/vocab/InC/1.0/ Aquatic Microbial Ecology - International Journal, 69(2) Zone polaire Polar region Beta-glucosidase Enzymatic activity Climatic zone Microbial community Microbial loop Glucide Extracellular Peptidases Extracellular enzymes Marine environment Hydrolases Fjord Chitinase Arctic Region Carbohydrate Communauté microbienne Glycosidases Enzyme Activité enzymatique Proteinase Boucle microbienne Extracellulaire Glycosylases Svalbard Article 2013 ftcarolinadr https://doi.org/10.17615/b8v7-7s82 2023-10-21T22:24:21Z Measurements of the spectrum of extracellular enzymes present in an environment can indicate the nature of organic substrates available to microorganisms. We report the activities in an Arctic fjord (Smeerenburgfjord, Svalbard) of the extracellular carbohydrate hydrolases a-galactosidase, β-glucosidase, and chitobiase, and the extracellular peptidases leucyl aminopeptidase, trypsin, and chymotrypsin. Among the carbohydrate hydrolases, β-glucosidase had the highest potential activity. Although extracellular leucyl aminopeptidase is frequently assayed in marine systems, activities of other peptidases have only rarely been reported. Peptidase activities were higher than carbohydrate hydrolase activities by approximately 2 orders of magnitude. Activities of leucyl aminopeptidase (an exopeptidase which cleaves terminal residues from a protein) were higher than trypsin and chymotrypsin (both endopeptidases which cleave interior bonds). In contrast to previous measurements from coastal, temperate environments, potential activity of leucyl aminopeptidase in Smeerenburg was higher than that of the endopeptidases trypsin and of chymotrypsin. These results suggest that leucyl aminopeptidase may not always be a reliable proxy for the total peptidolytic potential of microbial communities. Article in Journal/Newspaper Arctic Smeerenburg Smeerenburgfjord* Svalbard Carolina Digital Repository (UNC - University of North Carolina) Arctic Svalbard
institution Open Polar
collection Carolina Digital Repository (UNC - University of North Carolina)
op_collection_id ftcarolinadr
language English
topic Zone polaire
Polar region
Beta-glucosidase
Enzymatic activity
Climatic zone
Microbial community
Microbial loop
Glucide
Extracellular
Peptidases
Extracellular enzymes
Marine environment
Hydrolases
Fjord
Chitinase
Arctic Region
Carbohydrate
Communauté microbienne
Glycosidases
Enzyme
Activité enzymatique
Proteinase
Boucle microbienne
Extracellulaire
Glycosylases
Svalbard
spellingShingle Zone polaire
Polar region
Beta-glucosidase
Enzymatic activity
Climatic zone
Microbial community
Microbial loop
Glucide
Extracellular
Peptidases
Extracellular enzymes
Marine environment
Hydrolases
Fjord
Chitinase
Arctic Region
Carbohydrate
Communauté microbienne
Glycosidases
Enzyme
Activité enzymatique
Proteinase
Boucle microbienne
Extracellulaire
Glycosylases
Svalbard
Steen, Andrew D.
Arnosti, Carol
Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard)
topic_facet Zone polaire
Polar region
Beta-glucosidase
Enzymatic activity
Climatic zone
Microbial community
Microbial loop
Glucide
Extracellular
Peptidases
Extracellular enzymes
Marine environment
Hydrolases
Fjord
Chitinase
Arctic Region
Carbohydrate
Communauté microbienne
Glycosidases
Enzyme
Activité enzymatique
Proteinase
Boucle microbienne
Extracellulaire
Glycosylases
Svalbard
description Measurements of the spectrum of extracellular enzymes present in an environment can indicate the nature of organic substrates available to microorganisms. We report the activities in an Arctic fjord (Smeerenburgfjord, Svalbard) of the extracellular carbohydrate hydrolases a-galactosidase, β-glucosidase, and chitobiase, and the extracellular peptidases leucyl aminopeptidase, trypsin, and chymotrypsin. Among the carbohydrate hydrolases, β-glucosidase had the highest potential activity. Although extracellular leucyl aminopeptidase is frequently assayed in marine systems, activities of other peptidases have only rarely been reported. Peptidase activities were higher than carbohydrate hydrolase activities by approximately 2 orders of magnitude. Activities of leucyl aminopeptidase (an exopeptidase which cleaves terminal residues from a protein) were higher than trypsin and chymotrypsin (both endopeptidases which cleave interior bonds). In contrast to previous measurements from coastal, temperate environments, potential activity of leucyl aminopeptidase in Smeerenburg was higher than that of the endopeptidases trypsin and of chymotrypsin. These results suggest that leucyl aminopeptidase may not always be a reliable proxy for the total peptidolytic potential of microbial communities.
format Article in Journal/Newspaper
author Steen, Andrew D.
Arnosti, Carol
author_facet Steen, Andrew D.
Arnosti, Carol
author_sort Steen, Andrew D.
title Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard)
title_short Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard)
title_full Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard)
title_fullStr Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard)
title_full_unstemmed Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard)
title_sort extracellular peptidase and carbohydrate hydrolase activities in an arctic fjord (smeerenburgfjord, svalbard)
publishDate 2013
url https://doi.org/10.17615/b8v7-7s82
https://cdr.lib.unc.edu/downloads/k3569d269?file=thumbnail
https://cdr.lib.unc.edu/downloads/k3569d269
geographic Arctic
Svalbard
geographic_facet Arctic
Svalbard
genre Arctic
Smeerenburg
Smeerenburgfjord*
Svalbard
genre_facet Arctic
Smeerenburg
Smeerenburgfjord*
Svalbard
op_source Aquatic Microbial Ecology - International Journal, 69(2)
op_relation https://doi.org/10.17615/b8v7-7s82
https://cdr.lib.unc.edu/downloads/k3569d269?file=thumbnail
https://cdr.lib.unc.edu/downloads/k3569d269
op_rights http://rightsstatements.org/vocab/InC/1.0/
op_doi https://doi.org/10.17615/b8v7-7s82
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