MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears
Hibernating brown bears, Ursus arctos, undergo extended periods of inactivity and yet these large hibernators are resilient to muscle disuse atrophy. Physiological characteristics associated with atrophy resistance in bear muscle have been examined (e.g., muscle mechanics, neural activity) but roles...
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ftcarletonunivir:oai:carleton.ca:25665 2023-05-15T18:41:57+02:00 MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears Luu, B.E. (Bryan E.) Lefai, E. (Etienne) Giroud, S. (Sylvain) Swenson, J.E. (Jon E.) Chazarin, B. (Blandine) Gauquelin-Koch, G. (Guillemette) Arnemo, J.M. (Jon M.) Evans, A.L. (Alina L.) Bertile, F. (Fabrice) Storey, K. (Kenneth B.) 2019-01-01 https://ir.library.carleton.ca/pub/25665 https://doi.org/10.1002/jcp.29294 en eng https://ir.library.carleton.ca/pub/25665 doi:10.1002/jcp.29294 Journal of Cellular Physiology atrophy Mef2a myomiR noncoding RNA ubiquitin ligase Ursus arctos info:eu-repo/semantics/article 2019 ftcarletonunivir https://doi.org/10.1002/jcp.29294 2022-02-06T21:51:01Z Hibernating brown bears, Ursus arctos, undergo extended periods of inactivity and yet these large hibernators are resilient to muscle disuse atrophy. Physiological characteristics associated with atrophy resistance in bear muscle have been examined (e.g., muscle mechanics, neural activity) but roles for molecular signaling/regulatory mechanisms in the resistance to muscle wasting in bears still require investigation. Using quantitative reverse transcription PCR (RT-qPCR), the present study characterized the responses of 36 microRNAs linked with development, metabolism, and regeneration of skeletal muscle, in the vastus lateralis of brown bears comparing winter hibernating and summer active animals. Relative levels of mRNA of selected genes (mef2a, pax7, id2, prkaa1, and mstn) implicated upstream and downstream of the microRNAs were examined. Results indicated that hibernation elicited a myogenic microRNA, or “myomiR”, response via MEF2A-mediated signaling. Upregulation of MEF2A-controlled miR-1 and miR-206 and respective downregulation of pax7 and id2 mRNA are suggestive of responses that promote skeletal muscle maintenance. Increased levels of metabolic microRNAs, such as miR-27, miR-29, and miR-33, may facilitate metabolic suppression during hibernation via mechanisms that decrease glucose uptake and fatty acid oxidation. This study identified myomiR-mediated mechanisms for the promotion of muscle regeneration, suppression of ubiquitin ligases, and resistance to muscle atrophy during hibernation mediated by observed increases in miR-206, miR-221, miR-31, miR-23a, and miR-29b. This was further supported by the downregulation of myomiRs associated with a muscle injury and inflammation (miR-199a and miR-223) during hibernation. The present study provides evidence of myomiR-mediated signaling pathways that are activated during hibernation to maintain skeletal muscle functionality in brown bears. Article in Journal/Newspaper Ursus arctos Carleton University's Institutional Repository Journal of Cellular Physiology 235 4 3984 3993 |
institution |
Open Polar |
collection |
Carleton University's Institutional Repository |
op_collection_id |
ftcarletonunivir |
language |
English |
topic |
atrophy Mef2a myomiR noncoding RNA ubiquitin ligase Ursus arctos |
spellingShingle |
atrophy Mef2a myomiR noncoding RNA ubiquitin ligase Ursus arctos Luu, B.E. (Bryan E.) Lefai, E. (Etienne) Giroud, S. (Sylvain) Swenson, J.E. (Jon E.) Chazarin, B. (Blandine) Gauquelin-Koch, G. (Guillemette) Arnemo, J.M. (Jon M.) Evans, A.L. (Alina L.) Bertile, F. (Fabrice) Storey, K. (Kenneth B.) MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
topic_facet |
atrophy Mef2a myomiR noncoding RNA ubiquitin ligase Ursus arctos |
description |
Hibernating brown bears, Ursus arctos, undergo extended periods of inactivity and yet these large hibernators are resilient to muscle disuse atrophy. Physiological characteristics associated with atrophy resistance in bear muscle have been examined (e.g., muscle mechanics, neural activity) but roles for molecular signaling/regulatory mechanisms in the resistance to muscle wasting in bears still require investigation. Using quantitative reverse transcription PCR (RT-qPCR), the present study characterized the responses of 36 microRNAs linked with development, metabolism, and regeneration of skeletal muscle, in the vastus lateralis of brown bears comparing winter hibernating and summer active animals. Relative levels of mRNA of selected genes (mef2a, pax7, id2, prkaa1, and mstn) implicated upstream and downstream of the microRNAs were examined. Results indicated that hibernation elicited a myogenic microRNA, or “myomiR”, response via MEF2A-mediated signaling. Upregulation of MEF2A-controlled miR-1 and miR-206 and respective downregulation of pax7 and id2 mRNA are suggestive of responses that promote skeletal muscle maintenance. Increased levels of metabolic microRNAs, such as miR-27, miR-29, and miR-33, may facilitate metabolic suppression during hibernation via mechanisms that decrease glucose uptake and fatty acid oxidation. This study identified myomiR-mediated mechanisms for the promotion of muscle regeneration, suppression of ubiquitin ligases, and resistance to muscle atrophy during hibernation mediated by observed increases in miR-206, miR-221, miR-31, miR-23a, and miR-29b. This was further supported by the downregulation of myomiRs associated with a muscle injury and inflammation (miR-199a and miR-223) during hibernation. The present study provides evidence of myomiR-mediated signaling pathways that are activated during hibernation to maintain skeletal muscle functionality in brown bears. |
format |
Article in Journal/Newspaper |
author |
Luu, B.E. (Bryan E.) Lefai, E. (Etienne) Giroud, S. (Sylvain) Swenson, J.E. (Jon E.) Chazarin, B. (Blandine) Gauquelin-Koch, G. (Guillemette) Arnemo, J.M. (Jon M.) Evans, A.L. (Alina L.) Bertile, F. (Fabrice) Storey, K. (Kenneth B.) |
author_facet |
Luu, B.E. (Bryan E.) Lefai, E. (Etienne) Giroud, S. (Sylvain) Swenson, J.E. (Jon E.) Chazarin, B. (Blandine) Gauquelin-Koch, G. (Guillemette) Arnemo, J.M. (Jon M.) Evans, A.L. (Alina L.) Bertile, F. (Fabrice) Storey, K. (Kenneth B.) |
author_sort |
Luu, B.E. (Bryan E.) |
title |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_short |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_full |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_fullStr |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_full_unstemmed |
MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
title_sort |
micrornas facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears |
publishDate |
2019 |
url |
https://ir.library.carleton.ca/pub/25665 https://doi.org/10.1002/jcp.29294 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Journal of Cellular Physiology |
op_relation |
https://ir.library.carleton.ca/pub/25665 doi:10.1002/jcp.29294 |
op_doi |
https://doi.org/10.1002/jcp.29294 |
container_title |
Journal of Cellular Physiology |
container_volume |
235 |
container_issue |
4 |
container_start_page |
3984 |
op_container_end_page |
3993 |
_version_ |
1766231528910618624 |