Multivariate statistical analysis of metabolomics profiles in tissues of polar bears (Ursus maritimus) from the Southern and Western Hudson Bay subpopulations
Polar bears (Ursus maritimus) are apex predators of the Arctic, which exposes them to an array of natural and anthropogenic stress factors. Metabolomics analysis profiles endogenous metabolites that reflect the response of biological systems to stimuli, and the effects of multiple stressors can be a...
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ftcarletonunivir:oai:carleton.ca:15130 2023-05-15T15:10:42+02:00 Multivariate statistical analysis of metabolomics profiles in tissues of polar bears (Ursus maritimus) from the Southern and Western Hudson Bay subpopulations Morris, A.D. (A. D.) Letcher, R.J. (Robert J.) Dyck, M. (M.) Chandramouli, B. (B.) Fisk, A.T. (A. T.) Cosgrove, J. (J.) 2017-08-29 https://ir.library.carleton.ca/pub/15130 https://doi.org/10.1007/s00300-017-2200-6 en eng https://ir.library.carleton.ca/pub/15130 doi:10.1007/s00300-017-2200-6 Polar Biology, pp. 1-17 Hudson Bay Metabolomics Multivariate analysis Partial least squares discriminant analysis (PLS-DA) Polar bears (Ursus maritimus) info:eu-repo/semantics/article 2017 ftcarletonunivir https://doi.org/10.1007/s00300-017-2200-6 2022-02-06T21:50:15Z Polar bears (Ursus maritimus) are apex predators of the Arctic, which exposes them to an array of natural and anthropogenic stress factors. Metabolomics analysis profiles endogenous metabolites that reflect the response of biological systems to stimuli, and the effects of multiple stressors can be assessed from an integrated perspective. A targeted, quantitative, liquid chromatography–mass spectrometry-based metabolomics platform [219 metabolites including amino acids, biogenic amines, acylcarnitines, phosphatidylcholines (PCs), sphingomyelins, hexoses (Hex), and fatty acids (FAs)] was applied to the muscle and liver of polar bears from the Southern and Western Hudson Bay (Canada) subpopulations (SHB and WHB, respectively). Multivariate statistics were then applied to establish whether bears were discriminated by sex and/or subpopulation. Five metabolites identified by variable importance projection (VIP) discriminated the hepatic profiles of SHB males and females (Hex, arginine, glutamine, one PC, one sphingomyelin), while fifteen metabolites (primarily PCs along with leucine) contrasted the livers of males from SHB and WHB. Metabolite profiles in the muscle of male and female bears could not be differentiated; however, the muscles of SHB and WHB males were discriminated primarily by PCs and FAs. Stable isotope ratios (δ13C and δ15N) were variably related to metabolites; δ13C was correlated with some VIP metabolite concentrations, particularly in comparisons of male bears from SHB and WHB, suggesting an influence of dietary differences. However, δ15N and age exhibited few, relatively weak correlations wi Article in Journal/Newspaper Arctic Hudson Bay Polar Biology Ursus maritimus Carleton University's Institutional Repository Arctic Hudson Bay Canada Hudson Polar Biology 41 3 433 449 |
institution |
Open Polar |
collection |
Carleton University's Institutional Repository |
op_collection_id |
ftcarletonunivir |
language |
English |
topic |
Hudson Bay Metabolomics Multivariate analysis Partial least squares discriminant analysis (PLS-DA) Polar bears (Ursus maritimus) |
spellingShingle |
Hudson Bay Metabolomics Multivariate analysis Partial least squares discriminant analysis (PLS-DA) Polar bears (Ursus maritimus) Morris, A.D. (A. D.) Letcher, R.J. (Robert J.) Dyck, M. (M.) Chandramouli, B. (B.) Fisk, A.T. (A. T.) Cosgrove, J. (J.) Multivariate statistical analysis of metabolomics profiles in tissues of polar bears (Ursus maritimus) from the Southern and Western Hudson Bay subpopulations |
topic_facet |
Hudson Bay Metabolomics Multivariate analysis Partial least squares discriminant analysis (PLS-DA) Polar bears (Ursus maritimus) |
description |
Polar bears (Ursus maritimus) are apex predators of the Arctic, which exposes them to an array of natural and anthropogenic stress factors. Metabolomics analysis profiles endogenous metabolites that reflect the response of biological systems to stimuli, and the effects of multiple stressors can be assessed from an integrated perspective. A targeted, quantitative, liquid chromatography–mass spectrometry-based metabolomics platform [219 metabolites including amino acids, biogenic amines, acylcarnitines, phosphatidylcholines (PCs), sphingomyelins, hexoses (Hex), and fatty acids (FAs)] was applied to the muscle and liver of polar bears from the Southern and Western Hudson Bay (Canada) subpopulations (SHB and WHB, respectively). Multivariate statistics were then applied to establish whether bears were discriminated by sex and/or subpopulation. Five metabolites identified by variable importance projection (VIP) discriminated the hepatic profiles of SHB males and females (Hex, arginine, glutamine, one PC, one sphingomyelin), while fifteen metabolites (primarily PCs along with leucine) contrasted the livers of males from SHB and WHB. Metabolite profiles in the muscle of male and female bears could not be differentiated; however, the muscles of SHB and WHB males were discriminated primarily by PCs and FAs. Stable isotope ratios (δ13C and δ15N) were variably related to metabolites; δ13C was correlated with some VIP metabolite concentrations, particularly in comparisons of male bears from SHB and WHB, suggesting an influence of dietary differences. However, δ15N and age exhibited few, relatively weak correlations wi |
format |
Article in Journal/Newspaper |
author |
Morris, A.D. (A. D.) Letcher, R.J. (Robert J.) Dyck, M. (M.) Chandramouli, B. (B.) Fisk, A.T. (A. T.) Cosgrove, J. (J.) |
author_facet |
Morris, A.D. (A. D.) Letcher, R.J. (Robert J.) Dyck, M. (M.) Chandramouli, B. (B.) Fisk, A.T. (A. T.) Cosgrove, J. (J.) |
author_sort |
Morris, A.D. (A. D.) |
title |
Multivariate statistical analysis of metabolomics profiles in tissues of polar bears (Ursus maritimus) from the Southern and Western Hudson Bay subpopulations |
title_short |
Multivariate statistical analysis of metabolomics profiles in tissues of polar bears (Ursus maritimus) from the Southern and Western Hudson Bay subpopulations |
title_full |
Multivariate statistical analysis of metabolomics profiles in tissues of polar bears (Ursus maritimus) from the Southern and Western Hudson Bay subpopulations |
title_fullStr |
Multivariate statistical analysis of metabolomics profiles in tissues of polar bears (Ursus maritimus) from the Southern and Western Hudson Bay subpopulations |
title_full_unstemmed |
Multivariate statistical analysis of metabolomics profiles in tissues of polar bears (Ursus maritimus) from the Southern and Western Hudson Bay subpopulations |
title_sort |
multivariate statistical analysis of metabolomics profiles in tissues of polar bears (ursus maritimus) from the southern and western hudson bay subpopulations |
publishDate |
2017 |
url |
https://ir.library.carleton.ca/pub/15130 https://doi.org/10.1007/s00300-017-2200-6 |
geographic |
Arctic Hudson Bay Canada Hudson |
geographic_facet |
Arctic Hudson Bay Canada Hudson |
genre |
Arctic Hudson Bay Polar Biology Ursus maritimus |
genre_facet |
Arctic Hudson Bay Polar Biology Ursus maritimus |
op_source |
Polar Biology, pp. 1-17 |
op_relation |
https://ir.library.carleton.ca/pub/15130 doi:10.1007/s00300-017-2200-6 |
op_doi |
https://doi.org/10.1007/s00300-017-2200-6 |
container_title |
Polar Biology |
container_volume |
41 |
container_issue |
3 |
container_start_page |
433 |
op_container_end_page |
449 |
_version_ |
1766341681813127168 |