Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan
The occurrence of black fur, or melanism, in many mammalian species is known to be linked to DNA sequence variation in the agouti signaling protein (Asip) gene, which is a major determinant of eumelanin and pheomelanin pigments in coat color. We investigated 38 agouti (i.e., banded wildtype) and fou...
Published in: | Zoological Science |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2017
|
Subjects: | |
Online Access: | https://researchprofiles.canberra.edu.au/en/publications/6344e4ab-4f62-41f3-b4bd-e1f836fee7e5 https://doi.org/10.2108/zs170027 https://researchsystem.canberra.edu.au/ws/files/60819590/3811895850003996.pdf http://www.scopus.com/inward/record.url?scp=85045881295&partnerID=8YFLogxK |
id |
ftcanberrauncris:oai:pure.atira.dk:publications/6344e4ab-4f62-41f3-b4bd-e1f836fee7e5 |
---|---|
record_format |
openpolar |
spelling |
ftcanberrauncris:oai:pure.atira.dk:publications/6344e4ab-4f62-41f3-b4bd-e1f836fee7e5 2024-09-15T18:31:57+00:00 Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan Sasamori, Shoichi Wiewel, Andrew S. Thomson, Vicki A. Kobayashi, Motoko Nakata, Katsushi Suzuki, Hitoshi 2017-12 application/pdf https://researchprofiles.canberra.edu.au/en/publications/6344e4ab-4f62-41f3-b4bd-e1f836fee7e5 https://doi.org/10.2108/zs170027 https://researchsystem.canberra.edu.au/ws/files/60819590/3811895850003996.pdf http://www.scopus.com/inward/record.url?scp=85045881295&partnerID=8YFLogxK eng eng info:eu-repo/semantics/closedAccess Sasamori , S , Wiewel , A S , Thomson , V A , Kobayashi , M , Nakata , K & Suzuki , H 2017 , ' Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan ' , Zoological Science , vol. 34 , no. 6 , pp. 513-522 . https://doi.org/10.2108/zs170027 article 2017 ftcanberrauncris https://doi.org/10.2108/zs170027 2024-07-17T23:33:54Z The occurrence of black fur, or melanism, in many mammalian species is known to be linked to DNA sequence variation in the agouti signaling protein (Asip) gene, which is a major determinant of eumelanin and pheomelanin pigments in coat color. We investigated 38 agouti (i.e., banded wildtype) and four melanistic Rattus rattus species complex (RrC) lineage II specimens from Okinawa Island, Ryukyu Islands, Japan, for genetic variation in three exons and associated flanking regions in the Asip gene. On Okinawa, a predicted loss-of-function mutation caused by a cysteine to serine amino acid change at p.124C>S (c.370T>A) in the highly conserved functional domain of Asip was found in melanistic rats, but was absent in agouti specimens, suggesting that the p.124C>S mutation is responsible for the observed melanism. Phylogeographic analysis found that Asip sequences from Okinawan RrC lineage II, including both agouti and melanistic specimens, differed from: 1) both agouti and melanistic RrC lineage I from Otaru, Hokkaido, Japan, and 2) agouti RrC lineages I and II from South Australia. This suggests the possibility of in-situ mutation of the Asip gene, either within the RrC lineage II population on Okinawa or in an unsampled RrC lineage II population with biogeographic links to Okinawa, although incomplete lineage sorting could not be ruled out. Article in Journal/Newspaper Rattus rattus University of Canberra Research Portal Zoological Science 34 6 513 |
institution |
Open Polar |
collection |
University of Canberra Research Portal |
op_collection_id |
ftcanberrauncris |
language |
English |
description |
The occurrence of black fur, or melanism, in many mammalian species is known to be linked to DNA sequence variation in the agouti signaling protein (Asip) gene, which is a major determinant of eumelanin and pheomelanin pigments in coat color. We investigated 38 agouti (i.e., banded wildtype) and four melanistic Rattus rattus species complex (RrC) lineage II specimens from Okinawa Island, Ryukyu Islands, Japan, for genetic variation in three exons and associated flanking regions in the Asip gene. On Okinawa, a predicted loss-of-function mutation caused by a cysteine to serine amino acid change at p.124C>S (c.370T>A) in the highly conserved functional domain of Asip was found in melanistic rats, but was absent in agouti specimens, suggesting that the p.124C>S mutation is responsible for the observed melanism. Phylogeographic analysis found that Asip sequences from Okinawan RrC lineage II, including both agouti and melanistic specimens, differed from: 1) both agouti and melanistic RrC lineage I from Otaru, Hokkaido, Japan, and 2) agouti RrC lineages I and II from South Australia. This suggests the possibility of in-situ mutation of the Asip gene, either within the RrC lineage II population on Okinawa or in an unsampled RrC lineage II population with biogeographic links to Okinawa, although incomplete lineage sorting could not be ruled out. |
format |
Article in Journal/Newspaper |
author |
Sasamori, Shoichi Wiewel, Andrew S. Thomson, Vicki A. Kobayashi, Motoko Nakata, Katsushi Suzuki, Hitoshi |
spellingShingle |
Sasamori, Shoichi Wiewel, Andrew S. Thomson, Vicki A. Kobayashi, Motoko Nakata, Katsushi Suzuki, Hitoshi Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan |
author_facet |
Sasamori, Shoichi Wiewel, Andrew S. Thomson, Vicki A. Kobayashi, Motoko Nakata, Katsushi Suzuki, Hitoshi |
author_sort |
Sasamori, Shoichi |
title |
Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan |
title_short |
Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan |
title_full |
Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan |
title_fullStr |
Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan |
title_full_unstemmed |
Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan |
title_sort |
potential causative mutation for melanism in rats identified in the agouti signaling protein gene (asip) of the rattus rattus species complex on okinawa island, japan |
publishDate |
2017 |
url |
https://researchprofiles.canberra.edu.au/en/publications/6344e4ab-4f62-41f3-b4bd-e1f836fee7e5 https://doi.org/10.2108/zs170027 https://researchsystem.canberra.edu.au/ws/files/60819590/3811895850003996.pdf http://www.scopus.com/inward/record.url?scp=85045881295&partnerID=8YFLogxK |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Sasamori , S , Wiewel , A S , Thomson , V A , Kobayashi , M , Nakata , K & Suzuki , H 2017 , ' Potential Causative Mutation for Melanism in Rats Identified in the Agouti Signaling Protein Gene (Asip) of the Rattus rattus Species Complex on Okinawa Island, Japan ' , Zoological Science , vol. 34 , no. 6 , pp. 513-522 . https://doi.org/10.2108/zs170027 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.2108/zs170027 |
container_title |
Zoological Science |
container_volume |
34 |
container_issue |
6 |
container_start_page |
513 |
_version_ |
1810473688794923008 |