First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes)::insights into the karyotype evolution of this ancient fish order
Osteoglossiformes is one of the most ancient (Early Triassic) groups of freshwater teleosts and has at least one representative on each continent of the Southern Hemisphere, with the exception of Antarctica. Among its six described families, the Gymnarchidae occurs in many African rivers, including...
Published in: | Biological Journal of the Linnean Society |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | https://researchprofiles.canberra.edu.au/en/publications/1f89aa6a-a2e2-4e94-b07c-6d29a4a7d559 https://doi.org/10.1093/biolinnean/bly098 http://www.scopus.com/inward/record.url?scp=85054860856&partnerID=8YFLogxK http://www.mendeley.com/research/first-chromosomal-analysis-gymnarchus-niloticus-gymnarchidae-osteoglossiformes-insights-karyotype-ev-1 |
id |
ftcanberrauncris:oai:pure.atira.dk:publications/1f89aa6a-a2e2-4e94-b07c-6d29a4a7d559 |
---|---|
record_format |
openpolar |
spelling |
ftcanberrauncris:oai:pure.atira.dk:publications/1f89aa6a-a2e2-4e94-b07c-6d29a4a7d559 2023-05-15T13:38:46+02:00 First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes)::insights into the karyotype evolution of this ancient fish order Hatanaka, Terumi De Oliveira, Ezequiel Aguiar Ráb, Petr Yano, Cassia Fernanda Bertollo, Luiz Antônio Carlos EZAZ, Tariq Jegedi, Oladele OI Liehr, Thomas Olaleye, Victor F Cioffi, Marcelo DB 2018-07-20 https://researchprofiles.canberra.edu.au/en/publications/1f89aa6a-a2e2-4e94-b07c-6d29a4a7d559 https://doi.org/10.1093/biolinnean/bly098 http://www.scopus.com/inward/record.url?scp=85054860856&partnerID=8YFLogxK http://www.mendeley.com/research/first-chromosomal-analysis-gymnarchus-niloticus-gymnarchidae-osteoglossiformes-insights-karyotype-ev-1 eng eng info:eu-repo/semantics/closedAccess Hatanaka , T , De Oliveira , E A , Ráb , P , Yano , C F , Bertollo , L A C , EZAZ , T , Jegedi , O OI , Liehr , T , Olaleye , V F & Cioffi , M DB 2018 , ' First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes): insights into the karyotype evolution of this ancient fish order ' , Biological Journal of the Linnean Society , vol. 125 , no. 1 , pp. 83-92 . https://doi.org/10.1093/biolinnean/bly098 article 2018 ftcanberrauncris https://doi.org/10.1093/biolinnean/bly098 2022-10-16T06:19:28Z Osteoglossiformes is one of the most ancient (Early Triassic) groups of freshwater teleosts and has at least one representative on each continent of the Southern Hemisphere, with the exception of Antarctica. Among its six described families, the Gymnarchidae occurs in many African rivers, including the river Nile, where Gymnarchus niloticus is its only representative. In this study we provide, for the first time, a comprehensive cytogenetic analysis for G. niloticus, which includes definition of the standard karyotype, C-banding, repetitive DNA mapping and comparative genomic hybridization, in order to investigate its chromosomal patterns and provide new data for comparative analyses with other Osteoglossiformes species. The 2n was 34 (26 metacentric + 8 submetacentric) in both sexes, and C-positive heterochromatic bands were detected in the centromeric/pericentromeric regions of all chromosomes. The simultaneous detection of 18S and 5S ribosomal DNAs showed simple and non-syntenical locations for such sequences, and a Chromomycin A3-positive band was observed, which is co-localized within the 18S ribosomal RNA gene sequences. Fluorescence in situ hybridization using U2 small nuclear RNA, Rex6 and some microssatelites as probes showed a scattered distribution among chromosomes. Data from comparative genomic hybridization experiments with some other African Osteoglossiformes species showed that all these species have an advanced stage of sequence divergence. The results highlight the evolutionary diversification of osteoglossiform fishes. Article in Journal/Newspaper Antarc* Antarctica University of Canberra Research Portal Biological Journal of the Linnean Society 125 1 83 92 |
institution |
Open Polar |
collection |
University of Canberra Research Portal |
op_collection_id |
ftcanberrauncris |
language |
English |
description |
Osteoglossiformes is one of the most ancient (Early Triassic) groups of freshwater teleosts and has at least one representative on each continent of the Southern Hemisphere, with the exception of Antarctica. Among its six described families, the Gymnarchidae occurs in many African rivers, including the river Nile, where Gymnarchus niloticus is its only representative. In this study we provide, for the first time, a comprehensive cytogenetic analysis for G. niloticus, which includes definition of the standard karyotype, C-banding, repetitive DNA mapping and comparative genomic hybridization, in order to investigate its chromosomal patterns and provide new data for comparative analyses with other Osteoglossiformes species. The 2n was 34 (26 metacentric + 8 submetacentric) in both sexes, and C-positive heterochromatic bands were detected in the centromeric/pericentromeric regions of all chromosomes. The simultaneous detection of 18S and 5S ribosomal DNAs showed simple and non-syntenical locations for such sequences, and a Chromomycin A3-positive band was observed, which is co-localized within the 18S ribosomal RNA gene sequences. Fluorescence in situ hybridization using U2 small nuclear RNA, Rex6 and some microssatelites as probes showed a scattered distribution among chromosomes. Data from comparative genomic hybridization experiments with some other African Osteoglossiformes species showed that all these species have an advanced stage of sequence divergence. The results highlight the evolutionary diversification of osteoglossiform fishes. |
format |
Article in Journal/Newspaper |
author |
Hatanaka, Terumi De Oliveira, Ezequiel Aguiar Ráb, Petr Yano, Cassia Fernanda Bertollo, Luiz Antônio Carlos EZAZ, Tariq Jegedi, Oladele OI Liehr, Thomas Olaleye, Victor F Cioffi, Marcelo DB |
spellingShingle |
Hatanaka, Terumi De Oliveira, Ezequiel Aguiar Ráb, Petr Yano, Cassia Fernanda Bertollo, Luiz Antônio Carlos EZAZ, Tariq Jegedi, Oladele OI Liehr, Thomas Olaleye, Victor F Cioffi, Marcelo DB First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes)::insights into the karyotype evolution of this ancient fish order |
author_facet |
Hatanaka, Terumi De Oliveira, Ezequiel Aguiar Ráb, Petr Yano, Cassia Fernanda Bertollo, Luiz Antônio Carlos EZAZ, Tariq Jegedi, Oladele OI Liehr, Thomas Olaleye, Victor F Cioffi, Marcelo DB |
author_sort |
Hatanaka, Terumi |
title |
First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes)::insights into the karyotype evolution of this ancient fish order |
title_short |
First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes)::insights into the karyotype evolution of this ancient fish order |
title_full |
First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes)::insights into the karyotype evolution of this ancient fish order |
title_fullStr |
First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes)::insights into the karyotype evolution of this ancient fish order |
title_full_unstemmed |
First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes)::insights into the karyotype evolution of this ancient fish order |
title_sort |
first chromosomal analysis in gymnarchus niloticus (gymnarchidae: osteoglossiformes)::insights into the karyotype evolution of this ancient fish order |
publishDate |
2018 |
url |
https://researchprofiles.canberra.edu.au/en/publications/1f89aa6a-a2e2-4e94-b07c-6d29a4a7d559 https://doi.org/10.1093/biolinnean/bly098 http://www.scopus.com/inward/record.url?scp=85054860856&partnerID=8YFLogxK http://www.mendeley.com/research/first-chromosomal-analysis-gymnarchus-niloticus-gymnarchidae-osteoglossiformes-insights-karyotype-ev-1 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Hatanaka , T , De Oliveira , E A , Ráb , P , Yano , C F , Bertollo , L A C , EZAZ , T , Jegedi , O OI , Liehr , T , Olaleye , V F & Cioffi , M DB 2018 , ' First chromosomal analysis in Gymnarchus niloticus (Gymnarchidae: Osteoglossiformes): insights into the karyotype evolution of this ancient fish order ' , Biological Journal of the Linnean Society , vol. 125 , no. 1 , pp. 83-92 . https://doi.org/10.1093/biolinnean/bly098 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1093/biolinnean/bly098 |
container_title |
Biological Journal of the Linnean Society |
container_volume |
125 |
container_issue |
1 |
container_start_page |
83 |
op_container_end_page |
92 |
_version_ |
1766110947672326144 |