The role of iron in the ecology and physiology of marine bacteria /

• Main Author: Adly, Carol.
• Format: Thesis
• Language: English
• Published: McGill University 2005
• Subjects: Iron > Physiological transport; Iron > Metabolism; Heterotrophic bacteria; Marine bacteria; Pacific; Subarctic
• Online Access: http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97884

Despite being abundant in the earth's crust, the concentration of Fe in many oceanic regions is so low that it is limiting to the growth of photosynthetic plankton. Heterotrophic bacteria play key roles in the oceanic cycling of carbon and nutrients, but it is unclear whether they can be Fe-deficient in nature, or what possible effects Fe-deficiency might have on their ecology and physiology. In chapter 1, I investigated the response of a natural bacterial community to a mesoscale Fe-enrichment experiment in the northeast subarctic Pacific. The addition of Fe to surface waters caused a rapid stimulation of bacterial growth and production, and induced the organic Fe uptake systems of bacteria. These findings suggest that bacteria responded directly to increased Fe availability, and may be Fe-deficient in situ. In chapter 2, I examined the effects of Fe-deficiency on the coupled processes of carbon catabolism and adenosine triphosphate (ATP) production in cultures of the marine bacterium Pseudoalteromonas haloplanktis. In Fe-limited cells, Fe-dependent oxidative pathways of ATP production were downregulated, leading to an intracellular energy deficit. Thus, by altering carbon metabolism and energy acquisition of heterotrophic bacteria, Fe may affect the cycling of carbon in parts of the sea.