Biosynthesis of phenolic lipid models using oleyl alcohol and triolein

• Main Author: Lue, Bena-Marie
• Other Authors: Kermasha, S. (advisor)
• Format: Thesis
• Language: English
• Published: McGill University 2004
• Subjects: Phenols > Synthesis; Plant lipids > Synthesis; Lipase; Antarc*; Antarctica
• Online Access: http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80324

The overall objective of this study was the optimization of a model enzymatic system in organic solvent media for the biosynthesis of selected phenolic lipid compounds. The model enzymatic system consisted of cinnamic acid and oleyl alcohol as substrates using commercial immobilized lipase (Novozym 435) from Candida antarctica. The experimental findings showed that an increase in the hydrophobicity of the solvent mixture and a decrease in the aw values of the reaction medium increased the initial enzymatic activity and bioconversion yield; the use of an iso-octane and butanone solvent mixture (85:15, v/v) and an initial aw of 0.05 resulted in an initial enzymatic activity of 192.7 nmol product/g enzyme/min and a corresponding bioconversion yield of 95.3% after a 16-day reaction period.