Biogeneration of lipophenols by lipases using selected substrate models
The objective of the research was to carry out the biogeneration of lipophenols by enzymatic esterification of tricaprylin and caprylic acid with catechin and catechol in a model hexane system. Commercial lipases, including Lipase N from Rhizopus niveus, Lipozyme IM from Mucor miehei and Novozym 435...
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2003
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ftcanadathes:oai:collectionscanada.gc.ca:QMM.79113 2023-05-15T13:53:07+02:00 Biogeneration of lipophenols by lipases using selected substrate models Petel, Tamara Kermasha, Selim (advisor) Master of Science (Department of Food Science and Agricultural Chemistry.) 2003 application/pdf http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79113 en eng McGill University alephsysno: 001985015 proquestno: AAIMQ88282 Theses scanned by UMI/ProQuest. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79113 All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. Lipase Esterification Lipids in human nutrition Phenols Food -- Biotechnology Electronic Thesis or Dissertation 2003 ftcanadathes 2014-02-16T01:07:58Z The objective of the research was to carry out the biogeneration of lipophenols by enzymatic esterification of tricaprylin and caprylic acid with catechin and catechol in a model hexane system. Commercial lipases, including Lipase N from Rhizopus niveus, Lipozyme IM from Mucor miehei and Novozym 435 from Candida antarctica were used throughout this study. The effects of reaction time, incubation temperatures and agitation speeds on enzymatic hydrolytic activity were investigated to determine the optimal conditions for biocatalysis. The optimal temperatures for biocatalysis were determined to be 37.5°C for Lipase N, and 55°C for Lipozyme IM and Novozym 435; the optimum agitation speed was 100 rpm. Using Lipase N, maximum hydrolysis of 1.66 mumol free fatty acids/mL was obtained after 1.5 days of incubation, while with Lipozyme IM, maximum hydrolysis of 8.1 and 8.5 mumol free fatty acids/mL was obtained after 1 and 4 days, respectively. With Novozym 435, the highest hydrolysis of 4.0 and 6.1 mumol free fatty acids/mL were found after 2 and 9 days, respectively. Thesis Antarc* Antarctica Theses Canada/Thèses Canada (Library and Archives Canada) |
institution |
Open Polar |
collection |
Theses Canada/Thèses Canada (Library and Archives Canada) |
op_collection_id |
ftcanadathes |
language |
English |
topic |
Lipase Esterification Lipids in human nutrition Phenols Food -- Biotechnology |
spellingShingle |
Lipase Esterification Lipids in human nutrition Phenols Food -- Biotechnology Petel, Tamara Biogeneration of lipophenols by lipases using selected substrate models |
topic_facet |
Lipase Esterification Lipids in human nutrition Phenols Food -- Biotechnology |
description |
The objective of the research was to carry out the biogeneration of lipophenols by enzymatic esterification of tricaprylin and caprylic acid with catechin and catechol in a model hexane system. Commercial lipases, including Lipase N from Rhizopus niveus, Lipozyme IM from Mucor miehei and Novozym 435 from Candida antarctica were used throughout this study. The effects of reaction time, incubation temperatures and agitation speeds on enzymatic hydrolytic activity were investigated to determine the optimal conditions for biocatalysis. The optimal temperatures for biocatalysis were determined to be 37.5°C for Lipase N, and 55°C for Lipozyme IM and Novozym 435; the optimum agitation speed was 100 rpm. Using Lipase N, maximum hydrolysis of 1.66 mumol free fatty acids/mL was obtained after 1.5 days of incubation, while with Lipozyme IM, maximum hydrolysis of 8.1 and 8.5 mumol free fatty acids/mL was obtained after 1 and 4 days, respectively. With Novozym 435, the highest hydrolysis of 4.0 and 6.1 mumol free fatty acids/mL were found after 2 and 9 days, respectively. |
author2 |
Kermasha, Selim (advisor) |
format |
Thesis |
author |
Petel, Tamara |
author_facet |
Petel, Tamara |
author_sort |
Petel, Tamara |
title |
Biogeneration of lipophenols by lipases using selected substrate models |
title_short |
Biogeneration of lipophenols by lipases using selected substrate models |
title_full |
Biogeneration of lipophenols by lipases using selected substrate models |
title_fullStr |
Biogeneration of lipophenols by lipases using selected substrate models |
title_full_unstemmed |
Biogeneration of lipophenols by lipases using selected substrate models |
title_sort |
biogeneration of lipophenols by lipases using selected substrate models |
publisher |
McGill University |
publishDate |
2003 |
url |
http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79113 |
op_coverage |
Master of Science (Department of Food Science and Agricultural Chemistry.) |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
alephsysno: 001985015 proquestno: AAIMQ88282 Theses scanned by UMI/ProQuest. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79113 |
op_rights |
All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
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1766258070983278592 |