Characterization of mercury and selenium complex in ringed seal liver
Ringed seal (Phoca hispida) is a major component and a major source of mercury (Hg) in the Inuit traditional diet. A high correlation between Hg and selenium (Se), as different forms of Hg-Se complex, has been reported in many species of marine mammals. The chemical form of the Hg-Se complex in ring...
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ftcanadathes:oai:collectionscanada.gc.ca:QMM.31231 2023-05-15T16:55:13+02:00 Characterization of mercury and selenium complex in ringed seal liver Ha, Pengcheng, 1963- Chan, Laurie (advisor) Master of Science (School of Dietetics and Human Nutrition.) 2001 application/pdf http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=31231 en eng McGill University alephsysno: 001810502 proquestno: MQ70428 Theses scanned by UMI/ProQuest. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=31231 All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. Ringed seal -- Québec (Province) -- Kuujjuaq Region Ringed seal -- Northwest Territories Mercury -- Bioaccumulation Selenium -- Bioaccumulation Electronic Thesis or Dissertation 2001 ftcanadathes 2014-02-16T01:06:41Z Ringed seal (Phoca hispida) is a major component and a major source of mercury (Hg) in the Inuit traditional diet. A high correlation between Hg and selenium (Se), as different forms of Hg-Se complex, has been reported in many species of marine mammals. The chemical form of the Hg-Se complex in ringed seals has never be characterised. In this study, Hg and Se concentrations in different seal tissues: liver, kidney, muscle and brain, were measured. The highest Hg and Se concentrations were found in the liver and a strong linear correlation was also observed between Hg and Se concentration in the seal liver. Extensive chromatographic and mass spectrometry techniques have been used to isolate and characterize the Hg and Se ligands in seal liver. Extraction of Hg and Se showed that Hg and Se were bound to ligands within the cell membranes of ringed seal liver. The Hg/Se binding protein has a MW range of about 65 kDa and Hg and Se had a 1:1 molecular ratio. The Hg/Se binding protein may contain 3 major polypeptides with MW of 6510.8, 14305.1 and 14353.1 Da. The toxicology of this Hg/Se binding protein will be studied using an animal feeding experiment. Thesis inuit Kuujjuaq Northwest Territories Phoca hispida ringed seal Theses Canada/Thèses Canada (Library and Archives Canada) Kuujjuaq ENVELOPE(-68.398,-68.398,58.100,58.100) Northwest Territories |
institution |
Open Polar |
collection |
Theses Canada/Thèses Canada (Library and Archives Canada) |
op_collection_id |
ftcanadathes |
language |
English |
topic |
Ringed seal -- Québec (Province) -- Kuujjuaq Region Ringed seal -- Northwest Territories Mercury -- Bioaccumulation Selenium -- Bioaccumulation |
spellingShingle |
Ringed seal -- Québec (Province) -- Kuujjuaq Region Ringed seal -- Northwest Territories Mercury -- Bioaccumulation Selenium -- Bioaccumulation Ha, Pengcheng, 1963- Characterization of mercury and selenium complex in ringed seal liver |
topic_facet |
Ringed seal -- Québec (Province) -- Kuujjuaq Region Ringed seal -- Northwest Territories Mercury -- Bioaccumulation Selenium -- Bioaccumulation |
description |
Ringed seal (Phoca hispida) is a major component and a major source of mercury (Hg) in the Inuit traditional diet. A high correlation between Hg and selenium (Se), as different forms of Hg-Se complex, has been reported in many species of marine mammals. The chemical form of the Hg-Se complex in ringed seals has never be characterised. In this study, Hg and Se concentrations in different seal tissues: liver, kidney, muscle and brain, were measured. The highest Hg and Se concentrations were found in the liver and a strong linear correlation was also observed between Hg and Se concentration in the seal liver. Extensive chromatographic and mass spectrometry techniques have been used to isolate and characterize the Hg and Se ligands in seal liver. Extraction of Hg and Se showed that Hg and Se were bound to ligands within the cell membranes of ringed seal liver. The Hg/Se binding protein has a MW range of about 65 kDa and Hg and Se had a 1:1 molecular ratio. The Hg/Se binding protein may contain 3 major polypeptides with MW of 6510.8, 14305.1 and 14353.1 Da. The toxicology of this Hg/Se binding protein will be studied using an animal feeding experiment. |
author2 |
Chan, Laurie (advisor) |
format |
Thesis |
author |
Ha, Pengcheng, 1963- |
author_facet |
Ha, Pengcheng, 1963- |
author_sort |
Ha, Pengcheng, 1963- |
title |
Characterization of mercury and selenium complex in ringed seal liver |
title_short |
Characterization of mercury and selenium complex in ringed seal liver |
title_full |
Characterization of mercury and selenium complex in ringed seal liver |
title_fullStr |
Characterization of mercury and selenium complex in ringed seal liver |
title_full_unstemmed |
Characterization of mercury and selenium complex in ringed seal liver |
title_sort |
characterization of mercury and selenium complex in ringed seal liver |
publisher |
McGill University |
publishDate |
2001 |
url |
http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=31231 |
op_coverage |
Master of Science (School of Dietetics and Human Nutrition.) |
long_lat |
ENVELOPE(-68.398,-68.398,58.100,58.100) |
geographic |
Kuujjuaq Northwest Territories |
geographic_facet |
Kuujjuaq Northwest Territories |
genre |
inuit Kuujjuaq Northwest Territories Phoca hispida ringed seal |
genre_facet |
inuit Kuujjuaq Northwest Territories Phoca hispida ringed seal |
op_relation |
alephsysno: 001810502 proquestno: MQ70428 Theses scanned by UMI/ProQuest. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=31231 |
op_rights |
All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
_version_ |
1766046202111983616 |