Biodiversity and activity of microbial mat communities from Canadian high Arctic ice shelf ecosystems
Microbial mats exist in freshwater pools atop the Ward Hunt and Markham ice shelves in the Canadian high Arctic. In this study, culture-dependent and culture-independent techniques were used to describe the microbial diversity and activity of these mat communities.Bacterial and archaeal 16S rRNA gen...
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ftcanadathes:oai:collectionscanada.gc.ca:QMM.100776 2023-05-15T14:55:03+02:00 Biodiversity and activity of microbial mat communities from Canadian high Arctic ice shelf ecosystems Bottos, Eric. Master of Science (Department of Natural Resource Sciences.) 2007 application/pdf http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100776 en eng McGill University alephsysno: 002611980 proquestno: AAIMR32672 Theses scanned by UMI/ProQuest. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100776 © Eric Bottos, 2007 Microbial mats -- Canada Northern Microbial diversity -- Canada Electronic Thesis or Dissertation 2007 ftcanadathes 2014-02-16T01:05:35Z Microbial mats exist in freshwater pools atop the Ward Hunt and Markham ice shelves in the Canadian high Arctic. In this study, culture-dependent and culture-independent techniques were used to describe the microbial diversity and activity of these mat communities.Bacterial and archaeal 16S rRNA gene clone libraries were constructed from Markham and Ward Hunt mat samples. Bacterial libraries from both mats had high diversity, though the Markham library appeared more diverse than the Ward Hunt library. Over 95% of sequences in both bacterial libraries, and all isolates from both mats, grouped within the phyla Bacteroidetes, Proteobacteria, and Actinobacteria. Archaeal diversity appeared low in both mats. Only one phylotype, potentially representing a novel Euryarchaeota, was observed in both archaeal libraries. Populations in both communities showed activity at subzero temperatures, with growth of isolates at -5°C and detectable metabolic activity at -10°C, measured by radiorespiration assays of mat microcosms. Thesis Arctic Ice Shelf Ice Shelves Theses Canada/Thèses Canada (Library and Archives Canada) Arctic Canada Markham ENVELOPE(-57.358,-57.358,-64.296,-64.296) |
institution |
Open Polar |
collection |
Theses Canada/Thèses Canada (Library and Archives Canada) |
op_collection_id |
ftcanadathes |
language |
English |
topic |
Microbial mats -- Canada Northern Microbial diversity -- Canada |
spellingShingle |
Microbial mats -- Canada Northern Microbial diversity -- Canada Bottos, Eric. Biodiversity and activity of microbial mat communities from Canadian high Arctic ice shelf ecosystems |
topic_facet |
Microbial mats -- Canada Northern Microbial diversity -- Canada |
description |
Microbial mats exist in freshwater pools atop the Ward Hunt and Markham ice shelves in the Canadian high Arctic. In this study, culture-dependent and culture-independent techniques were used to describe the microbial diversity and activity of these mat communities.Bacterial and archaeal 16S rRNA gene clone libraries were constructed from Markham and Ward Hunt mat samples. Bacterial libraries from both mats had high diversity, though the Markham library appeared more diverse than the Ward Hunt library. Over 95% of sequences in both bacterial libraries, and all isolates from both mats, grouped within the phyla Bacteroidetes, Proteobacteria, and Actinobacteria. Archaeal diversity appeared low in both mats. Only one phylotype, potentially representing a novel Euryarchaeota, was observed in both archaeal libraries. Populations in both communities showed activity at subzero temperatures, with growth of isolates at -5°C and detectable metabolic activity at -10°C, measured by radiorespiration assays of mat microcosms. |
format |
Thesis |
author |
Bottos, Eric. |
author_facet |
Bottos, Eric. |
author_sort |
Bottos, Eric. |
title |
Biodiversity and activity of microbial mat communities from Canadian high Arctic ice shelf ecosystems |
title_short |
Biodiversity and activity of microbial mat communities from Canadian high Arctic ice shelf ecosystems |
title_full |
Biodiversity and activity of microbial mat communities from Canadian high Arctic ice shelf ecosystems |
title_fullStr |
Biodiversity and activity of microbial mat communities from Canadian high Arctic ice shelf ecosystems |
title_full_unstemmed |
Biodiversity and activity of microbial mat communities from Canadian high Arctic ice shelf ecosystems |
title_sort |
biodiversity and activity of microbial mat communities from canadian high arctic ice shelf ecosystems |
publisher |
McGill University |
publishDate |
2007 |
url |
http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100776 |
op_coverage |
Master of Science (Department of Natural Resource Sciences.) |
long_lat |
ENVELOPE(-57.358,-57.358,-64.296,-64.296) |
geographic |
Arctic Canada Markham |
geographic_facet |
Arctic Canada Markham |
genre |
Arctic Ice Shelf Ice Shelves |
genre_facet |
Arctic Ice Shelf Ice Shelves |
op_relation |
alephsysno: 002611980 proquestno: AAIMR32672 Theses scanned by UMI/ProQuest. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100776 |
op_rights |
© Eric Bottos, 2007 |
_version_ |
1766326831945875456 |