Genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, Coregonus clupeaformis (Mitchill) in Western Canada

Genetic studies were undertaken to establish the bases of four electrophoretically polymorphic proteins in lake whitefish, Coregonus clupeaformis. These biochemical characters were then used to investigate zoogeographic problems in lake whitefish which had been posed as a result of morphological stu...

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Main Author: Franzin, William Gilbert.
Language:unknown
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/1993/6153
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spelling ftcanadathes:oai:collectionscanada.gc.ca:MWU.1993/6153 2023-05-15T18:45:58+02:00 Genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, Coregonus clupeaformis (Mitchill) in Western Canada Franzin, William Gilbert. 2012-05-15T15:54:16Z http://hdl.handle.net/1993/6153 unknown http://hdl.handle.net/1993/6153 2012 ftcanadathes 2014-03-30T00:51:21Z Genetic studies were undertaken to establish the bases of four electrophoretically polymorphic proteins in lake whitefish, Coregonus clupeaformis. These biochemical characters were then used to investigate zoogeographic problems in lake whitefish which had been posed as a result of morphological study by other workers. Breeding experiments revealed the genetics of the electrophoretic phenotypes of glycerol-3-phosphate dehydrogenase (G-3-PDH) isozymes from white muscle. The G-3-PDH isozyme phenotypes were explained on the basis of a molecular, genetic model involving two loci, one having two alleles and the second three alleles. This model predicted, through simple Mendelian non-dominance, a total of eighteen phenotypes, fifteen of which were observed among 2200 lake whitefish from 38 lakes in Western Canada. Additional genetic information was derived from examination of lake whitefish muscle for phenotypes of lactate dehydrogenase (LDH) produced by a heart-type LDH locus. This locus is represented by two alleles, the genetics of which were determined in an earlier study (Clayton and Franzin, 1970). Less precise information was obtained from study of malate dehydrogenase (MDH) and hemoglobin phenotypes. All biochemical data were used collectively to test an hypothesis, developed from morphological evidence, that lake whitefish existed in at least two refugia (Bering and Mississippi) during the Wisconsin glaciation, and that the variability seen among lake whitefish populations in Western Canada is at least partly due to postglacial admixture of two or more discrete stocks. The biochemical observations all revealed a break in gene frequencies at the periphery of the Yukon River watershed which is roughly consistent with morphological observations. Biochemical data suggest that gene flow in lake whitefish has been unidirectionally out of the Yukon River watershed. Other/Unknown Material Yukon river Yukon Theses Canada/Thèses Canada (Library and Archives Canada) Canada Clayton ENVELOPE(-64.183,-64.183,-65.167,-65.167) Yukon
institution Open Polar
collection Theses Canada/Thèses Canada (Library and Archives Canada)
op_collection_id ftcanadathes
language unknown
description Genetic studies were undertaken to establish the bases of four electrophoretically polymorphic proteins in lake whitefish, Coregonus clupeaformis. These biochemical characters were then used to investigate zoogeographic problems in lake whitefish which had been posed as a result of morphological study by other workers. Breeding experiments revealed the genetics of the electrophoretic phenotypes of glycerol-3-phosphate dehydrogenase (G-3-PDH) isozymes from white muscle. The G-3-PDH isozyme phenotypes were explained on the basis of a molecular, genetic model involving two loci, one having two alleles and the second three alleles. This model predicted, through simple Mendelian non-dominance, a total of eighteen phenotypes, fifteen of which were observed among 2200 lake whitefish from 38 lakes in Western Canada. Additional genetic information was derived from examination of lake whitefish muscle for phenotypes of lactate dehydrogenase (LDH) produced by a heart-type LDH locus. This locus is represented by two alleles, the genetics of which were determined in an earlier study (Clayton and Franzin, 1970). Less precise information was obtained from study of malate dehydrogenase (MDH) and hemoglobin phenotypes. All biochemical data were used collectively to test an hypothesis, developed from morphological evidence, that lake whitefish existed in at least two refugia (Bering and Mississippi) during the Wisconsin glaciation, and that the variability seen among lake whitefish populations in Western Canada is at least partly due to postglacial admixture of two or more discrete stocks. The biochemical observations all revealed a break in gene frequencies at the periphery of the Yukon River watershed which is roughly consistent with morphological observations. Biochemical data suggest that gene flow in lake whitefish has been unidirectionally out of the Yukon River watershed.
author Franzin, William Gilbert.
spellingShingle Franzin, William Gilbert.
Genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, Coregonus clupeaformis (Mitchill) in Western Canada
author_facet Franzin, William Gilbert.
author_sort Franzin, William Gilbert.
title Genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, Coregonus clupeaformis (Mitchill) in Western Canada
title_short Genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, Coregonus clupeaformis (Mitchill) in Western Canada
title_full Genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, Coregonus clupeaformis (Mitchill) in Western Canada
title_fullStr Genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, Coregonus clupeaformis (Mitchill) in Western Canada
title_full_unstemmed Genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, Coregonus clupeaformis (Mitchill) in Western Canada
title_sort genetic studies of protein variants and their use in a zoogeographic study of lake whitefish, coregonus clupeaformis (mitchill) in western canada
publishDate 2012
url http://hdl.handle.net/1993/6153
long_lat ENVELOPE(-64.183,-64.183,-65.167,-65.167)
geographic Canada
Clayton
Yukon
geographic_facet Canada
Clayton
Yukon
genre Yukon river
Yukon
genre_facet Yukon river
Yukon
op_relation http://hdl.handle.net/1993/6153
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