lip10-2 (+ megacomplex)
Exposure 1-1.3s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magn...
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ftcaltechdata:oai:data.caltech.edu:y8n82-m4v76 2023-10-01T03:54:27+02:00 lip10-2 (+ megacomplex) Nicolas, William 2020 mp4 jpg mrc rec https://doi.org/10.22002/y8n82-m4v76 eng eng CaltechDATA wnj2020-09-29-47 doi:10.22002/y8n82-m4v76 oai:data.caltech.edu:y8n82-m4v76 info:eu-repo/semantics/openAccess Creative Commons Attribution Non Commercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/legalcode Escherichia coli PK2 BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor info:eu-repo/semantics/other 2020 ftcaltechdata https://doi.org/10.22002/y8n82-m4v76 2023-09-03T20:52:42Z Exposure 1-1.3s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: BL21 cells were transformed with QAB - pCDF plasmid and EFG - pRSF . plasmid to express the inner-membrane macro-complex. . Selection done with Kanamycin and Streptomycin. . Induction of expression ON done with IPTG. . Spheroplasts are generated from the BL21 non transformed cells cells. Spheroplasts are processed through a high-pressure homogenizer and . further purification by ultracentrifugation of the membrane . fractions. . Liposomes are then generated from the membrane fractions by . processing through Potter and sonicator. Sample Preparation: Fiducials were prepared my method (see lab archive). . Grids: Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL pipetted on the grids Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-47 lip10-2-sq3-tg18.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download lip10-2-sq3-tg18_full.rec, Reconstruction (Pixel Size 0.78 nm), 1.8 GB Download Other/Unknown Material artica CaltechDATA (California Institute of Technology Research Data Repository) |
institution |
Open Polar |
collection |
CaltechDATA (California Institute of Technology Research Data Repository) |
op_collection_id |
ftcaltechdata |
language |
English |
topic |
Escherichia coli PK2 BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor |
spellingShingle |
Escherichia coli PK2 BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor Nicolas, William lip10-2 (+ megacomplex) |
topic_facet |
Escherichia coli PK2 BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor |
description |
Exposure 1-1.3s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: BL21 cells were transformed with QAB - pCDF plasmid and EFG - pRSF . plasmid to express the inner-membrane macro-complex. . Selection done with Kanamycin and Streptomycin. . Induction of expression ON done with IPTG. . Spheroplasts are generated from the BL21 non transformed cells cells. Spheroplasts are processed through a high-pressure homogenizer and . further purification by ultracentrifugation of the membrane . fractions. . Liposomes are then generated from the membrane fractions by . processing through Potter and sonicator. Sample Preparation: Fiducials were prepared my method (see lab archive). . Grids: Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL pipetted on the grids Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-47 lip10-2-sq3-tg18.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download lip10-2-sq3-tg18_full.rec, Reconstruction (Pixel Size 0.78 nm), 1.8 GB Download |
format |
Other/Unknown Material |
author |
Nicolas, William |
author_facet |
Nicolas, William |
author_sort |
Nicolas, William |
title |
lip10-2 (+ megacomplex) |
title_short |
lip10-2 (+ megacomplex) |
title_full |
lip10-2 (+ megacomplex) |
title_fullStr |
lip10-2 (+ megacomplex) |
title_full_unstemmed |
lip10-2 (+ megacomplex) |
title_sort |
lip10-2 (+ megacomplex) |
publisher |
CaltechDATA |
publishDate |
2020 |
url |
https://doi.org/10.22002/y8n82-m4v76 |
genre |
artica |
genre_facet |
artica |
op_relation |
wnj2020-09-29-47 doi:10.22002/y8n82-m4v76 oai:data.caltech.edu:y8n82-m4v76 |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution Non Commercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/legalcode |
op_doi |
https://doi.org/10.22002/y8n82-m4v76 |
_version_ |
1778522123086594048 |