lip8-4 (ctrl)

Exposure 1s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magnific...

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Bibliographic Details
Main Author: Nicolas, William
Format: Other/Unknown Material
Language:English
Published: CaltechDATA 2020
Subjects:
Escherichia coli
PK2
BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
artica
Online Access:https://doi.org/10.22002/xpbpe-47v36
id ftcaltechdata:oai:data.caltech.edu:xpbpe-47v36
record_format openpolar
spelling ftcaltechdata:oai:data.caltech.edu:xpbpe-47v36 2023-10-09T21:49:41+02:00 lip8-4 (ctrl) Nicolas, William 2020 mp4 jpg mrc rec https://doi.org/10.22002/xpbpe-47v36 eng eng CaltechDATA wnj2020-09-29-54 oai:data.caltech.edu:xpbpe-47v36 doi:10.22002/xpbpe-47v36 info:eu-repo/semantics/openAccess Creative Commons Attribution Non Commercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/legalcode Escherichia coli PK2 BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor info:eu-repo/semantics/other 2020 ftcaltechdata https://doi.org/10.22002/xpbpe-47v36 2023-09-10T20:44:42Z Exposure 1s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: . . Spheroplasts are generated from the BL21 non transformed cells . cells. Spheroplasts are processed through a high-pressure . homogenizer and further purification by . ultracentrifugation of the . membrane fractions. . Liposomes are then generated from the membrane . fractions by processing through Potter and sonicator. Sample Preparation: Fiducials were prepared my method (see lab archive). . Grids: . Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL . pipetted on the grids Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-54 lip8-4_sq1_tg2.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download lip8-4_sq1_tg2_full.rec, Reconstruction (Pixel Size 0.78 nm), 1.8 GB Download Other/Unknown Material artica CaltechDATA (California Institute of Technology Research Data Repository)
institution Open Polar
collection CaltechDATA (California Institute of Technology Research Data Repository)
op_collection_id ftcaltechdata
language English
topic Escherichia coli
PK2
BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
spellingShingle Escherichia coli
PK2
BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
Nicolas, William
lip8-4 (ctrl)
topic_facet Escherichia coli
PK2
BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
description Exposure 1s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: . . Spheroplasts are generated from the BL21 non transformed cells . cells. Spheroplasts are processed through a high-pressure . homogenizer and further purification by . ultracentrifugation of the . membrane fractions. . Liposomes are then generated from the membrane . fractions by processing through Potter and sonicator. Sample Preparation: Fiducials were prepared my method (see lab archive). . Grids: . Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL . pipetted on the grids Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-54 lip8-4_sq1_tg2.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download lip8-4_sq1_tg2_full.rec, Reconstruction (Pixel Size 0.78 nm), 1.8 GB Download
format Other/Unknown Material
author Nicolas, William
author_facet Nicolas, William
author_sort Nicolas, William
title lip8-4 (ctrl)
title_short lip8-4 (ctrl)
title_full lip8-4 (ctrl)
title_fullStr lip8-4 (ctrl)
title_full_unstemmed lip8-4 (ctrl)
title_sort lip8-4 (ctrl)
publisher CaltechDATA
publishDate 2020
url https://doi.org/10.22002/xpbpe-47v36
genre artica
genre_facet artica
op_relation wnj2020-09-29-54
oai:data.caltech.edu:xpbpe-47v36
doi:10.22002/xpbpe-47v36
op_rights info:eu-repo/semantics/openAccess
Creative Commons Attribution Non Commercial 4.0 International
https://creativecommons.org/licenses/by-nc/4.0/legalcode
op_doi https://doi.org/10.22002/xpbpe-47v36
_version_ 1779312715150393344

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