lip10-2 (+ megacomplex)

Exposure 1-1.3s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magn...

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Bibliographic Details
Main Author: Nicolas, William
Format: Other/Unknown Material
Language:English
Published: CaltechDATA 2020
Subjects:
Escherichia coli
PK2
BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
artica
Online Access:https://doi.org/10.22002/c6p8n-dy013
id ftcaltechdata:oai:data.caltech.edu:c6p8n-dy013
record_format openpolar
spelling ftcaltechdata:oai:data.caltech.edu:c6p8n-dy013 2023-10-01T03:54:27+02:00 lip10-2 (+ megacomplex) Nicolas, William 2020 mp4 jpg mrc rec https://doi.org/10.22002/c6p8n-dy013 eng eng CaltechDATA wnj2020-09-29-36 doi:10.22002/c6p8n-dy013 oai:data.caltech.edu:c6p8n-dy013 info:eu-repo/semantics/openAccess Creative Commons Attribution Non Commercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/legalcode Escherichia coli PK2 BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor info:eu-repo/semantics/other 2020 ftcaltechdata https://doi.org/10.22002/c6p8n-dy013 2023-09-03T21:24:46Z Exposure 1-1.3s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: BL21 cells were transformed with QAB - pCDF plasmid and EFG - pRSF . plasmid to express the inner-membrane macro-complex. . Selection done with Kanamycin and Streptomycin. . Induction of expression ON done with IPTG. . Spheroplasts are generated from the BL21 non transformed cells cells. Spheroplasts are processed through a high-pressure homogenizer and . further purification by ultracentrifugation of the membrane . fractions. . Liposomes are then generated from the membrane fractions by . processing through Potter and sonicator. Sample Preparation: Fiducials were prepared my method (see lab archive). . Grids: Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL pipetted on the grids Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-36 lip10-2-sq3-tg9.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download lip10-2-sq3-tg9_full.rec, Reconstruction (Pixel Size 0.78 nm), 1.8 GB Download Other/Unknown Material artica CaltechDATA (California Institute of Technology Research Data Repository)
institution Open Polar
collection CaltechDATA (California Institute of Technology Research Data Repository)
op_collection_id ftcaltechdata
language English
topic Escherichia coli
PK2
BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
spellingShingle Escherichia coli
PK2
BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
Nicolas, William
lip10-2 (+ megacomplex)
topic_facet Escherichia coli
PK2
BL21 dHisdHA RQABpCDF+EFGpRSF
Single Axis
IECB Talos Artica
Serial EM
pipeline
Raptor
description Exposure 1-1.3s Tilt Series Date: 2020-09-29 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -8.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: BL21 cells were transformed with QAB - pCDF plasmid and EFG - pRSF . plasmid to express the inner-membrane macro-complex. . Selection done with Kanamycin and Streptomycin. . Induction of expression ON done with IPTG. . Spheroplasts are generated from the BL21 non transformed cells cells. Spheroplasts are processed through a high-pressure homogenizer and . further purification by ultracentrifugation of the membrane . fractions. . Liposomes are then generated from the membrane fractions by . processing through Potter and sonicator. Sample Preparation: Fiducials were prepared my method (see lab archive). . Grids: Quantifoil Cu NH2 R2/2. . Glowdischarged 3mA - 2min. BT3s - BF0. 4uL pipetted on the grids Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2020-09-29-36 lip10-2-sq3-tg9.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download lip10-2-sq3-tg9_full.rec, Reconstruction (Pixel Size 0.78 nm), 1.8 GB Download
format Other/Unknown Material
author Nicolas, William
author_facet Nicolas, William
author_sort Nicolas, William
title lip10-2 (+ megacomplex)
title_short lip10-2 (+ megacomplex)
title_full lip10-2 (+ megacomplex)
title_fullStr lip10-2 (+ megacomplex)
title_full_unstemmed lip10-2 (+ megacomplex)
title_sort lip10-2 (+ megacomplex)
publisher CaltechDATA
publishDate 2020
url https://doi.org/10.22002/c6p8n-dy013
genre artica
genre_facet artica
op_relation wnj2020-09-29-36
doi:10.22002/c6p8n-dy013
oai:data.caltech.edu:c6p8n-dy013
op_rights info:eu-repo/semantics/openAccess
Creative Commons Attribution Non Commercial 4.0 International
https://creativecommons.org/licenses/by-nc/4.0/legalcode
op_doi https://doi.org/10.22002/c6p8n-dy013
_version_ 1778522123928600576

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