Folding of Deoxymyoglobin Triggered by Electron Transfer
The met and deoxy forms of sperm whale myoglobin (Mb) can be unfolded by guanidine hydrochloride (GuHCl). Electronic absorption and circular dichroism spectroscopic measurements show that folded deoxyMb is more stable than the folded met protein. Laser excitation of NADH generates species that rapid...
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American Chemical Society
1998
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Online Access: | https://doi.org/10.1021/jp9802228 |
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ftcaltechauth:oai:authors.library.caltech.edu:g2eze-j8f85 2024-06-23T07:56:58+00:00 Folding of Deoxymyoglobin Triggered by Electron Transfer Wittung-Stafshede, Pernilla Malmström, Bo G. Winkler, Jay R. Gray, Harry B. 1998-07-09 https://doi.org/10.1021/jp9802228 unknown American Chemical Society https://doi.org/10.1021/jp9802228 oai:authors.library.caltech.edu:g2eze-j8f85 eprintid:85293 resolverid:CaltechAUTHORS:20180313-160333459 info:eu-repo/semantics/closedAccess Other Journal of Physical Chemistry A, 102(28), 5599-5601, (1998-07-09) info:eu-repo/semantics/article 1998 ftcaltechauth https://doi.org/10.1021/jp9802228 2024-06-12T02:13:56Z The met and deoxy forms of sperm whale myoglobin (Mb) can be unfolded by guanidine hydrochloride (GuHCl). Electronic absorption and circular dichroism spectroscopic measurements show that folded deoxyMb is more stable than the folded met protein. Laser excitation of NADH generates species that rapidly reduce unfolded metMb, triggering the formation of folded deoxyMb in less than 10 ms (pH 7.0, 2.5 to 3 M GuHCl, 20 °C). At comparable reaction driving forces (∼10 kJ/mol), deoxyMb folds much faster than reduced cytochrome c. © 1998 American Chemical Society. Received: November 17, 1997; In Final Form: April 1, 1998. Publication Date (Web): May 23, 1998. P.W.-S. thanks the Swedish Technical Research Council for a postdoctoral fellowship and Ivan Dmochowski for assistance with the diode array measurements. This work was supported by the National Science Foundation (H.B.G., J.R.W.), the Nobel Institute for Chemistry (B.G.M.), and the Arnold and Mabel Beckman Foundation. Article in Journal/Newspaper Sperm whale Caltech Authors (California Institute of Technology) Mabel ENVELOPE(-44.683,-44.683,-60.667,-60.667) The Journal of Physical Chemistry A 102 28 5599 5601 |
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Caltech Authors (California Institute of Technology) |
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The met and deoxy forms of sperm whale myoglobin (Mb) can be unfolded by guanidine hydrochloride (GuHCl). Electronic absorption and circular dichroism spectroscopic measurements show that folded deoxyMb is more stable than the folded met protein. Laser excitation of NADH generates species that rapidly reduce unfolded metMb, triggering the formation of folded deoxyMb in less than 10 ms (pH 7.0, 2.5 to 3 M GuHCl, 20 °C). At comparable reaction driving forces (∼10 kJ/mol), deoxyMb folds much faster than reduced cytochrome c. © 1998 American Chemical Society. Received: November 17, 1997; In Final Form: April 1, 1998. Publication Date (Web): May 23, 1998. P.W.-S. thanks the Swedish Technical Research Council for a postdoctoral fellowship and Ivan Dmochowski for assistance with the diode array measurements. This work was supported by the National Science Foundation (H.B.G., J.R.W.), the Nobel Institute for Chemistry (B.G.M.), and the Arnold and Mabel Beckman Foundation. |
format |
Article in Journal/Newspaper |
author |
Wittung-Stafshede, Pernilla Malmström, Bo G. Winkler, Jay R. Gray, Harry B. |
spellingShingle |
Wittung-Stafshede, Pernilla Malmström, Bo G. Winkler, Jay R. Gray, Harry B. Folding of Deoxymyoglobin Triggered by Electron Transfer |
author_facet |
Wittung-Stafshede, Pernilla Malmström, Bo G. Winkler, Jay R. Gray, Harry B. |
author_sort |
Wittung-Stafshede, Pernilla |
title |
Folding of Deoxymyoglobin Triggered by Electron Transfer |
title_short |
Folding of Deoxymyoglobin Triggered by Electron Transfer |
title_full |
Folding of Deoxymyoglobin Triggered by Electron Transfer |
title_fullStr |
Folding of Deoxymyoglobin Triggered by Electron Transfer |
title_full_unstemmed |
Folding of Deoxymyoglobin Triggered by Electron Transfer |
title_sort |
folding of deoxymyoglobin triggered by electron transfer |
publisher |
American Chemical Society |
publishDate |
1998 |
url |
https://doi.org/10.1021/jp9802228 |
long_lat |
ENVELOPE(-44.683,-44.683,-60.667,-60.667) |
geographic |
Mabel |
geographic_facet |
Mabel |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Journal of Physical Chemistry A, 102(28), 5599-5601, (1998-07-09) |
op_relation |
https://doi.org/10.1021/jp9802228 oai:authors.library.caltech.edu:g2eze-j8f85 eprintid:85293 resolverid:CaltechAUTHORS:20180313-160333459 |
op_rights |
info:eu-repo/semantics/closedAccess Other |
op_doi |
https://doi.org/10.1021/jp9802228 |
container_title |
The Journal of Physical Chemistry A |
container_volume |
102 |
container_issue |
28 |
container_start_page |
5599 |
op_container_end_page |
5601 |
_version_ |
1802650387023396864 |