Hydrophobic mismatch in gramicidin A'/Lecithin systems
Gramicidin A’ (GA’) has been added to three lipid systems of varying hydrophobic thicknesses: dimyristoyllecithin (DML), dipalmitoyllecithin (DPL), and distearoyllecithin (DSL). The similarity in length between the hydrophobic portion of GA’ and the hydrocarbon chains of the lipid bilayers has been...
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American Chemical Society
1990
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ftcaltechauth:oai:authors.library.caltech.edu:84796 2023-05-15T16:01:35+02:00 Hydrophobic mismatch in gramicidin A'/Lecithin systems Watnick, Paula I. Chan, Sunney I. Dea, Phoebe 1990-07-03 https://authors.library.caltech.edu/84796/ https://resolver.caltech.edu/CaltechAUTHORS:20180212-141345237 unknown American Chemical Society Watnick, Paula I. and Chan, Sunney I. and Dea, Phoebe (1990) Hydrophobic mismatch in gramicidin A'/Lecithin systems. Biochemistry, 29 (26). pp. 6215-6221. ISSN 0006-2960. doi:10.1021/bi00478a015. https://resolver.caltech.edu/CaltechAUTHORS:20180212-141345237 <https://resolver.caltech.edu/CaltechAUTHORS:20180212-141345237> Article PeerReviewed 1990 ftcaltechauth https://doi.org/10.1021/bi00478a015 2021-11-18T18:45:00Z Gramicidin A’ (GA’) has been added to three lipid systems of varying hydrophobic thicknesses: dimyristoyllecithin (DML), dipalmitoyllecithin (DPL), and distearoyllecithin (DSL). The similarity in length between the hydrophobic portion of GA’ and the hydrocarbon chains of the lipid bilayers has been studied by using ^(31)P and ^2H NMR. Hydrophobic mismatch has been found to be most severe in the DML bilayer system and minimal in the case of DSL. In addition, the effects of hydrophobic mismatch on the cooperative properties of the bilayer have been obtained from ^2H NMR relaxation measurements. The results indicate that incorporation of the peptide into the bilayer disrupts the cooperative director fluctuations characteristic of pure multilamellar lipid dispersions. Finally, the GA’llecithin ratio at which the well-known transformation from bilayer to reverse hexagonal (H_(II)) phase occurs (Van Echteld et al., 1982; Chupin et al., 1987) is shown to depend on the acyl chain length of the phospholipid. A rationale is proposed for this chain length dependence. Article in Journal/Newspaper DML Caltech Authors (California Institute of Technology) Biochemistry 29 26 6215 6221 |
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Caltech Authors (California Institute of Technology) |
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Gramicidin A’ (GA’) has been added to three lipid systems of varying hydrophobic thicknesses: dimyristoyllecithin (DML), dipalmitoyllecithin (DPL), and distearoyllecithin (DSL). The similarity in length between the hydrophobic portion of GA’ and the hydrocarbon chains of the lipid bilayers has been studied by using ^(31)P and ^2H NMR. Hydrophobic mismatch has been found to be most severe in the DML bilayer system and minimal in the case of DSL. In addition, the effects of hydrophobic mismatch on the cooperative properties of the bilayer have been obtained from ^2H NMR relaxation measurements. The results indicate that incorporation of the peptide into the bilayer disrupts the cooperative director fluctuations characteristic of pure multilamellar lipid dispersions. Finally, the GA’llecithin ratio at which the well-known transformation from bilayer to reverse hexagonal (H_(II)) phase occurs (Van Echteld et al., 1982; Chupin et al., 1987) is shown to depend on the acyl chain length of the phospholipid. A rationale is proposed for this chain length dependence. |
format |
Article in Journal/Newspaper |
author |
Watnick, Paula I. Chan, Sunney I. Dea, Phoebe |
spellingShingle |
Watnick, Paula I. Chan, Sunney I. Dea, Phoebe Hydrophobic mismatch in gramicidin A'/Lecithin systems |
author_facet |
Watnick, Paula I. Chan, Sunney I. Dea, Phoebe |
author_sort |
Watnick, Paula I. |
title |
Hydrophobic mismatch in gramicidin A'/Lecithin systems |
title_short |
Hydrophobic mismatch in gramicidin A'/Lecithin systems |
title_full |
Hydrophobic mismatch in gramicidin A'/Lecithin systems |
title_fullStr |
Hydrophobic mismatch in gramicidin A'/Lecithin systems |
title_full_unstemmed |
Hydrophobic mismatch in gramicidin A'/Lecithin systems |
title_sort |
hydrophobic mismatch in gramicidin a'/lecithin systems |
publisher |
American Chemical Society |
publishDate |
1990 |
url |
https://authors.library.caltech.edu/84796/ https://resolver.caltech.edu/CaltechAUTHORS:20180212-141345237 |
genre |
DML |
genre_facet |
DML |
op_relation |
Watnick, Paula I. and Chan, Sunney I. and Dea, Phoebe (1990) Hydrophobic mismatch in gramicidin A'/Lecithin systems. Biochemistry, 29 (26). pp. 6215-6221. ISSN 0006-2960. doi:10.1021/bi00478a015. https://resolver.caltech.edu/CaltechAUTHORS:20180212-141345237 <https://resolver.caltech.edu/CaltechAUTHORS:20180212-141345237> |
op_doi |
https://doi.org/10.1021/bi00478a015 |
container_title |
Biochemistry |
container_volume |
29 |
container_issue |
26 |
container_start_page |
6215 |
op_container_end_page |
6221 |
_version_ |
1766397379491135488 |