Anaerobic Degradation of Alkanes by Marine Archaea

• Published in: Annual Review of Microbiology
• Main Authors: Wegener, Gunter, Laso-Pérez, Rafael, Orphan, Victoria J., Boetius, Antje
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Annual Reviews 2022
• Subjects: archaea; alkanes; methyl coenzyme M reductase; anaerobic metabolism; microbial consortia; syntrophy; Microbiology; Chadwick; Alfred Wegener Institute
• Online Access: https://doi.org/10.1146/annurev-micro-111021-045911

Alkanes are saturated apolar hydrocarbons that range from their simplest form, methane, to high-molecular-weight compounds. Although alkanes were once considered biologically recalcitrant under anaerobic conditions, microbiological investigations have now identified several microbial taxa that can anaerobically degrade alkanes. Here we review recent discoveries in the anaerobic oxidation of alkanes with a specific focus on archaea that use specific methyl coenzyme M reductases to activate their substrates. Our understanding of the diversity of uncultured alkane-oxidizing archaea has expanded through the use of environmental metagenomics and enrichment cultures of syntrophic methane-, ethane-, propane-, and butane-oxidizing marine archaea with sulfate-reducing bacteria. A recently cultured group of archaea directly couples long-chain alkane degradation with methane formation, expanding the range of substrates used for methanogenesis. This article summarizes the rapidly growing knowledge of the diversity, physiology, and habitat distribution of alkane-degrading archaea. This review was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Initiative/Strategy through the Cluster of Excellence "The Ocean Floor-Earth's Uncharted Interface" (EXC-2077–390741603 to G.W., R.L.-P., A.B., and V.J.O.). Further support came from the Max Planck Institute for Marine Microbiology and the Alfred Wegener Institute Helmholtz Center for Polar and Marine Research. V.J.O.'s contributions were supported by the Department of Energy (DE-SC0020373) and by the Symbiosis Model Systems Initiative of the Gordon and Betty Moore Foundation. V.J.O. is a fellow of the Canadian Institute for Advanced Research (CIFAR) in the Earth 4D program. We thank C. Hahn, H. Yu, G. Chadwick, Y. Guo, C. Welte, A. Arshad, J. Kurth, and F. Musat for providing microscopy images. This article was prepared in the memory of the methane pioneers William S. Reeburgh and Ronald S. Oremland, who have inspired ...