Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris)
Northern elephant seals (Mirounga angustirostris) (NES) are known to be deep, long-duration divers and to sustain long-repeated patterns of breath-hold, or apnea. Some phocid dives remain within the bounds of aerobic metabolism, accompanied by physiological responses inducing lung compression, brady...
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Frontiers in Physiology
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ftcalifstateuniv:oai:scholarworks:w6634422w 2024-09-30T14:34:22+00:00 Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris) Colby Moore Daniel E. Crocker Andreas Fahlman Michael Moore Darryn S. Willoughby Kathleen Robbins Shane B. Kanatous Stephen J. Trumble 2014-05 http://hdl.handle.net/10211.3/121347 English eng Frontiers in Physiology Funding was provided by the Baylor University Faculty Research Investment Program (SJT). http://hdl.handle.net/10211.3/121347 ischemia reperfusion injury elephant seal diving fiber typing myoglobin Article 2014 ftcalifstateuniv 2024-09-10T17:06:15Z Northern elephant seals (Mirounga angustirostris) (NES) are known to be deep, long-duration divers and to sustain long-repeated patterns of breath-hold, or apnea. Some phocid dives remain within the bounds of aerobic metabolism, accompanied by physiological responses inducing lung compression, bradycardia and peripheral vasoconstriction. Current data suggest an absence of type IIb fibers in pinniped locomotory musculature. To date, no fiber type data exist for NES, a consummate deep diver. In this study, NES were biopsied in the wild. Ontogenetic changes in skeletal muscle were revealed through succinate dehydrogenase (SDH) based fiber typing. Results indicated a predominance of uniformly shaped, large type I fibers and elevated myoglobin (Mb) concentrations in the longissimus dorsi (LD) muscle of adults. No type II muscle fibers were detected in any adult sampled. This was in contrast to the juvenile animals that demonstrated type II myosin in Western Blot analysis, indicative of an ontogenetic change in skeletal muscle with maturation. These data support previous hypotheses that the absence of type II fibers indicates reliance on aerobic metabolism during dives, as well as a depressed metabolic rate and low energy locomotion. We also suggest that the lack of type IIb fibers (adults) may provide a protection against ischemia reperfusion (IR) injury in vasoconstricted peripheral skeletal muscle. This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission. The definitive version of this article is available at: http://www.frontiersin.org/physiology Moore, C., Crocker, D.E., Fahlman, A., Moore, M., Willoughby, D.S., Robbins, K., Kanatous, S.B., Trumble, S.J. "Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris)." Frontiers in Physiology 5.217 (2014) 1664-042X Article in Journal/Newspaper Elephant Seal Elephant Seals Scholarworks from California State University Nes ENVELOPE(7.634,7.634,62.795,62.795) Nes’ ENVELOPE(44.681,44.681,66.600,66.600) |
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Open Polar |
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Scholarworks from California State University |
op_collection_id |
ftcalifstateuniv |
language |
English |
topic |
ischemia reperfusion injury elephant seal diving fiber typing myoglobin |
spellingShingle |
ischemia reperfusion injury elephant seal diving fiber typing myoglobin Colby Moore Daniel E. Crocker Andreas Fahlman Michael Moore Darryn S. Willoughby Kathleen Robbins Shane B. Kanatous Stephen J. Trumble Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris) |
topic_facet |
ischemia reperfusion injury elephant seal diving fiber typing myoglobin |
description |
Northern elephant seals (Mirounga angustirostris) (NES) are known to be deep, long-duration divers and to sustain long-repeated patterns of breath-hold, or apnea. Some phocid dives remain within the bounds of aerobic metabolism, accompanied by physiological responses inducing lung compression, bradycardia and peripheral vasoconstriction. Current data suggest an absence of type IIb fibers in pinniped locomotory musculature. To date, no fiber type data exist for NES, a consummate deep diver. In this study, NES were biopsied in the wild. Ontogenetic changes in skeletal muscle were revealed through succinate dehydrogenase (SDH) based fiber typing. Results indicated a predominance of uniformly shaped, large type I fibers and elevated myoglobin (Mb) concentrations in the longissimus dorsi (LD) muscle of adults. No type II muscle fibers were detected in any adult sampled. This was in contrast to the juvenile animals that demonstrated type II myosin in Western Blot analysis, indicative of an ontogenetic change in skeletal muscle with maturation. These data support previous hypotheses that the absence of type II fibers indicates reliance on aerobic metabolism during dives, as well as a depressed metabolic rate and low energy locomotion. We also suggest that the lack of type IIb fibers (adults) may provide a protection against ischemia reperfusion (IR) injury in vasoconstricted peripheral skeletal muscle. This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission. The definitive version of this article is available at: http://www.frontiersin.org/physiology Moore, C., Crocker, D.E., Fahlman, A., Moore, M., Willoughby, D.S., Robbins, K., Kanatous, S.B., Trumble, S.J. "Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris)." Frontiers in Physiology 5.217 (2014) 1664-042X |
format |
Article in Journal/Newspaper |
author |
Colby Moore Daniel E. Crocker Andreas Fahlman Michael Moore Darryn S. Willoughby Kathleen Robbins Shane B. Kanatous Stephen J. Trumble |
author_facet |
Colby Moore Daniel E. Crocker Andreas Fahlman Michael Moore Darryn S. Willoughby Kathleen Robbins Shane B. Kanatous Stephen J. Trumble |
author_sort |
Colby Moore |
title |
Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris) |
title_short |
Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris) |
title_full |
Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris) |
title_fullStr |
Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris) |
title_full_unstemmed |
Ontogenetic Changes in Skeletal Muscle Fiber Type, Fiber Diameter and Myoglobin Concentration in the Northern Elephant Seal (Mirounga angustirostris) |
title_sort |
ontogenetic changes in skeletal muscle fiber type, fiber diameter and myoglobin concentration in the northern elephant seal (mirounga angustirostris) |
publisher |
Frontiers in Physiology |
publishDate |
2014 |
url |
http://hdl.handle.net/10211.3/121347 |
long_lat |
ENVELOPE(7.634,7.634,62.795,62.795) ENVELOPE(44.681,44.681,66.600,66.600) |
geographic |
Nes Nes’ |
geographic_facet |
Nes Nes’ |
genre |
Elephant Seal Elephant Seals |
genre_facet |
Elephant Seal Elephant Seals |
op_relation |
http://hdl.handle.net/10211.3/121347 |
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1811637996348243968 |