Bioaccumulation of polychlorinated biphenyls (PCBs) and dichlorodiphenylethane (DDE) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims
Polychlorinated biphenyl ( PCB) and dichlorodiphenylethane (DDE) methyl sulfone (MSF) metabolites possess high affinities for binding two homologous 16,000 Da homodimeric receptor proteins in the lung (Clara cell secretory protein, CCSP) and the uterus (uteroglobin, UG) , leading to selective bioacc...
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ftbruneluniv:oai:bura.brunel.ac.uk:2438/9184 2023-05-15T16:33:10+02:00 Bioaccumulation of polychlorinated biphenyls (PCBs) and dichlorodiphenylethane (DDE) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims Troisi, GM Haraguchi, K Kaydoo, DS Nyman, M Aguilar, A Borrell, A Siebert, U Mason, CF 2001 http://bura.brunel.ac.uk/handle/2438/9184 http://dx.doi.10.1080/00984100050201622 en eng Taylor & Francis Journal of Toxicology and Environmental Health, Part A, 62:1–8, 2001 1528-7394 http://bura.brunel.ac.uk/handle/2438/9184 http://dx.doi.10.1080/00984100050201622 Marine mammals Polychlorinated biphenyls (PCBs) Methyl sulphones (MSFs) DDE (primary DDT metabolite) Uteroglobin Article 2001 ftbruneluniv 2021-08-08T08:34:43Z Polychlorinated biphenyl ( PCB) and dichlorodiphenylethane (DDE) methyl sulfone (MSF) metabolites possess high affinities for binding two homologous 16,000 Da homodimeric receptor proteins in the lung (Clara cell secretory protein, CCSP) and the uterus (uteroglobin, UG) , leading to selective bioaccumulation of MSFs in these tissues. As marine mammals are highly exposed to organochlorines, concentrations of PCBs, PCB MSFs, DDT, and DDE MSF were analyzed in blubber, lung, and uterus samples from harbor seal ( Phoca vitulina) and striped dolphin ( Stenella coeruleoalba) morbillivirus epizootic victims to investigate uterine and lung MSF accumulation. Mean uterus concentrations of PCB MSFs and DDE MSF in harbor seals were 0.61 and 0.04 μg/ g lipid weight and in striped dolphins 0.05 and 0.01 μg/ g lipid weight. Mean lung concentrations of PCB MSFs and DDE MSF in harbor seals were 0.96 and 0.02 μg/ g lipid weight and in striped dolphins 0.16 and 0.01 μg/ g lipid weight. To ascertain whether uterine and lung bioaccumulation of MSFs is possible due to the presence of CCSP and UG in seals, CCSP and UG proteins in uterine flushings and in uterine and lung and epithelial tissue from Baltic gray and ringed seals were characterized using gel electrophoresis and Western blotting techniques. UG- and CCSP-like proteins with molecular weights of 16,000 Da were resolved in all samples. This is the first demonstration of this protein in any marine mammalian species. The toxicological implications of MSF binding with UG and CCSP in marine mammals are discussed. Care for the Wild (UK) Article in Journal/Newspaper harbor seal Phoca vitulina Brunel University London: Brunel University Research Archive (BURA) |
institution |
Open Polar |
collection |
Brunel University London: Brunel University Research Archive (BURA) |
op_collection_id |
ftbruneluniv |
language |
English |
topic |
Marine mammals Polychlorinated biphenyls (PCBs) Methyl sulphones (MSFs) DDE (primary DDT metabolite) Uteroglobin |
spellingShingle |
Marine mammals Polychlorinated biphenyls (PCBs) Methyl sulphones (MSFs) DDE (primary DDT metabolite) Uteroglobin Troisi, GM Haraguchi, K Kaydoo, DS Nyman, M Aguilar, A Borrell, A Siebert, U Mason, CF Bioaccumulation of polychlorinated biphenyls (PCBs) and dichlorodiphenylethane (DDE) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims |
topic_facet |
Marine mammals Polychlorinated biphenyls (PCBs) Methyl sulphones (MSFs) DDE (primary DDT metabolite) Uteroglobin |
description |
Polychlorinated biphenyl ( PCB) and dichlorodiphenylethane (DDE) methyl sulfone (MSF) metabolites possess high affinities for binding two homologous 16,000 Da homodimeric receptor proteins in the lung (Clara cell secretory protein, CCSP) and the uterus (uteroglobin, UG) , leading to selective bioaccumulation of MSFs in these tissues. As marine mammals are highly exposed to organochlorines, concentrations of PCBs, PCB MSFs, DDT, and DDE MSF were analyzed in blubber, lung, and uterus samples from harbor seal ( Phoca vitulina) and striped dolphin ( Stenella coeruleoalba) morbillivirus epizootic victims to investigate uterine and lung MSF accumulation. Mean uterus concentrations of PCB MSFs and DDE MSF in harbor seals were 0.61 and 0.04 μg/ g lipid weight and in striped dolphins 0.05 and 0.01 μg/ g lipid weight. Mean lung concentrations of PCB MSFs and DDE MSF in harbor seals were 0.96 and 0.02 μg/ g lipid weight and in striped dolphins 0.16 and 0.01 μg/ g lipid weight. To ascertain whether uterine and lung bioaccumulation of MSFs is possible due to the presence of CCSP and UG in seals, CCSP and UG proteins in uterine flushings and in uterine and lung and epithelial tissue from Baltic gray and ringed seals were characterized using gel electrophoresis and Western blotting techniques. UG- and CCSP-like proteins with molecular weights of 16,000 Da were resolved in all samples. This is the first demonstration of this protein in any marine mammalian species. The toxicological implications of MSF binding with UG and CCSP in marine mammals are discussed. Care for the Wild (UK) |
format |
Article in Journal/Newspaper |
author |
Troisi, GM Haraguchi, K Kaydoo, DS Nyman, M Aguilar, A Borrell, A Siebert, U Mason, CF |
author_facet |
Troisi, GM Haraguchi, K Kaydoo, DS Nyman, M Aguilar, A Borrell, A Siebert, U Mason, CF |
author_sort |
Troisi, GM |
title |
Bioaccumulation of polychlorinated biphenyls (PCBs) and dichlorodiphenylethane (DDE) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims |
title_short |
Bioaccumulation of polychlorinated biphenyls (PCBs) and dichlorodiphenylethane (DDE) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims |
title_full |
Bioaccumulation of polychlorinated biphenyls (PCBs) and dichlorodiphenylethane (DDE) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims |
title_fullStr |
Bioaccumulation of polychlorinated biphenyls (PCBs) and dichlorodiphenylethane (DDE) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims |
title_full_unstemmed |
Bioaccumulation of polychlorinated biphenyls (PCBs) and dichlorodiphenylethane (DDE) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims |
title_sort |
bioaccumulation of polychlorinated biphenyls (pcbs) and dichlorodiphenylethane (dde) methyl sulfones in tissues of seal and dolphin morbillivirus epizootic victims |
publisher |
Taylor & Francis |
publishDate |
2001 |
url |
http://bura.brunel.ac.uk/handle/2438/9184 http://dx.doi.10.1080/00984100050201622 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_relation |
Journal of Toxicology and Environmental Health, Part A, 62:1–8, 2001 1528-7394 http://bura.brunel.ac.uk/handle/2438/9184 http://dx.doi.10.1080/00984100050201622 |
_version_ |
1766022875479801856 |