Local Efforts to Expand the World’s Largest Trichomycete Collection
Trichomycetes (gut fungi) are obligate symbionts of various arthropods and have been found in marine, freshwater, and terrestrial habitats on every continent except Antarctica. Gut fungi associate commensally with their immature aquatic hosts (including black flies, mayflies, stoneflies, isopods, an...
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ftboisestateu:oai:scholarworks.boisestate.edu:as_12-1070 2023-10-29T02:30:30+01:00 Local Efforts to Expand the World’s Largest Trichomycete Collection Gause, Justin W. Wilson, Emma Reynolds, Nicole Sugden, Luke Chamberlin, Alison White, Merlin 2012-04-16T07:00:00Z https://scholarworks.boisestate.edu/as_12/71 unknown ScholarWorks https://scholarworks.boisestate.edu/as_12/71 College of Arts and Sciences Poster Presentations text 2012 ftboisestateu 2023-09-29T15:00:52Z Trichomycetes (gut fungi) are obligate symbionts of various arthropods and have been found in marine, freshwater, and terrestrial habitats on every continent except Antarctica. Gut fungi associate commensally with their immature aquatic hosts (including black flies, mayflies, stoneflies, isopods, and others) attaching to the chitinous lining of the mid- or hindgut. Gut fungi are routinely isolated from the host’s digestive tract into axenic cultures, which provide a rich resource of genetic material for phylogenic analysis. Given the obligate endosymbiotic nature of the fungi, unique challenges are encountered upon culturing, thus only 30% of all trichomycetes have been successfully cultured. Our laboratory houses the world’s largest culture collection of trichomycetes. We have been endeavoring to expand the collection by formulating suitable media in which field collected trichomycete specimens can grow. This requires attention to variables such as competing bacterial growth, pH changes required for sporulation, agar density and proper nutrient balance within the media. The continued development and enhancement of culture media is intended to increase the number of isolates that previously were considered "unculturable" and offer more robust samples of genetic material for future analyses. Text Antarc* Antarctica Boise State University: Scholar Works |
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Open Polar |
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Boise State University: Scholar Works |
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ftboisestateu |
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unknown |
description |
Trichomycetes (gut fungi) are obligate symbionts of various arthropods and have been found in marine, freshwater, and terrestrial habitats on every continent except Antarctica. Gut fungi associate commensally with their immature aquatic hosts (including black flies, mayflies, stoneflies, isopods, and others) attaching to the chitinous lining of the mid- or hindgut. Gut fungi are routinely isolated from the host’s digestive tract into axenic cultures, which provide a rich resource of genetic material for phylogenic analysis. Given the obligate endosymbiotic nature of the fungi, unique challenges are encountered upon culturing, thus only 30% of all trichomycetes have been successfully cultured. Our laboratory houses the world’s largest culture collection of trichomycetes. We have been endeavoring to expand the collection by formulating suitable media in which field collected trichomycete specimens can grow. This requires attention to variables such as competing bacterial growth, pH changes required for sporulation, agar density and proper nutrient balance within the media. The continued development and enhancement of culture media is intended to increase the number of isolates that previously were considered "unculturable" and offer more robust samples of genetic material for future analyses. |
format |
Text |
author |
Gause, Justin W. Wilson, Emma Reynolds, Nicole Sugden, Luke Chamberlin, Alison White, Merlin |
spellingShingle |
Gause, Justin W. Wilson, Emma Reynolds, Nicole Sugden, Luke Chamberlin, Alison White, Merlin Local Efforts to Expand the World’s Largest Trichomycete Collection |
author_facet |
Gause, Justin W. Wilson, Emma Reynolds, Nicole Sugden, Luke Chamberlin, Alison White, Merlin |
author_sort |
Gause, Justin W. |
title |
Local Efforts to Expand the World’s Largest Trichomycete Collection |
title_short |
Local Efforts to Expand the World’s Largest Trichomycete Collection |
title_full |
Local Efforts to Expand the World’s Largest Trichomycete Collection |
title_fullStr |
Local Efforts to Expand the World’s Largest Trichomycete Collection |
title_full_unstemmed |
Local Efforts to Expand the World’s Largest Trichomycete Collection |
title_sort |
local efforts to expand the world’s largest trichomycete collection |
publisher |
ScholarWorks |
publishDate |
2012 |
url |
https://scholarworks.boisestate.edu/as_12/71 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
College of Arts and Sciences Poster Presentations |
op_relation |
https://scholarworks.boisestate.edu/as_12/71 |
_version_ |
1781058946334720000 |