The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids
Several lipases from microbial and plant, i.e Rhizomucor miehei, Pseudomonas sp., Candida antartica, rice bran, and Carica papaya latex (CPL) were examined for synthesis of omega-3 (n-3) PUFA-rich glyceride by hydrolysis and acidolysis reaction. Tuna oil was used in hydrolysis reaction, whereas tuna...
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Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia
2010
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ftbogoraunivojs:oai:ojs.journal.ipb.ac.id:article/820 2024-09-15T17:49:15+00:00 The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids Elisabeth, Jenny Yuliani, T Tambunan, P M Purba, J M 2010-05-18 http://journal.ipb.ac.id/index.php/jtip/article/view/820 en eng Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia http://journal.ipb.ac.id/index.php/jtip/article/view/820 Jurnal Teknologi dan Industri Pangan; Vol. 12 No. 1 (2001): Jurnal Teknologi dan Industri Pangan 2087-751X 1979-7788 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2010 ftbogoraunivojs 2024-08-13T04:17:55Z Several lipases from microbial and plant, i.e Rhizomucor miehei, Pseudomonas sp., Candida antartica, rice bran, and Carica papaya latex (CPL) were examined for synthesis of omega-3 (n-3) PUFA-rich glyceride by hydrolysis and acidolysis reaction. Tuna oil was used in hydrolysis reaction, whereas tuna and palm oils were used as source of triglyceride (TAG) molecules and n-3 PUFA concentrate from tuna oil as source of EPA and DHA in acidolysis reaction.For hydrolysis reaction, the rice bran and CPL lipases showed the lowest hydrolytic activity of the tuna oil, whereas the R. miehei lipase showed the highest hydrolytic activity but was unable to hydrolyze EPA and DHA. On the contrary, the C. antartica and Pseudomonas sp. lipases acted stronger on hydrolysis of DHA ester bond than EPA.For acidolysis reaction, all the lipases showed ability to incorporate n-3 PUFA into tuna and palm oils. C. antartica lipase had the maximum DHA incorporation into tuna and palm oils, rice bran lipase had relatively similar ability with R. miehei lipase, and the CPL lipase had the lowest ability. This study proved that rice bran and CPL lipases also had transesterification activity and showed the feasibility of the rice bran lipase to be a biocatalyst for n-3 PUFA-rich glyceride production. Increasing the substrate ratio, of n-3 PUFA concentrate and tuna or palm oil, could increase the EPA and DHA incorporation. The R. miehei, rice bran, and CPL lipases unabled to incorporate DHA into DHA-containing glyceride molecule, whereas C. antartica lipase had the capability in high ratio of n-3 PUFA concentrate to oil. Therefore, the lipases were easier to incorporate n-3 PUFA into palm oil than tuna oil, since the TAG molecules of palm oil was not as complex as tuna oil. It could be suggested that the lipases did not only have acyl chain and positional specificity, but also the whole glyceride structure specificity.Keywords : acidolysis, lipase, n-3 PUFA, palm oil, specificity, tuna oil. Article in Journal/Newspaper antartic* Scientific Journals of Bogor Agricultural University |
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Open Polar |
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Scientific Journals of Bogor Agricultural University |
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ftbogoraunivojs |
language |
English |
description |
Several lipases from microbial and plant, i.e Rhizomucor miehei, Pseudomonas sp., Candida antartica, rice bran, and Carica papaya latex (CPL) were examined for synthesis of omega-3 (n-3) PUFA-rich glyceride by hydrolysis and acidolysis reaction. Tuna oil was used in hydrolysis reaction, whereas tuna and palm oils were used as source of triglyceride (TAG) molecules and n-3 PUFA concentrate from tuna oil as source of EPA and DHA in acidolysis reaction.For hydrolysis reaction, the rice bran and CPL lipases showed the lowest hydrolytic activity of the tuna oil, whereas the R. miehei lipase showed the highest hydrolytic activity but was unable to hydrolyze EPA and DHA. On the contrary, the C. antartica and Pseudomonas sp. lipases acted stronger on hydrolysis of DHA ester bond than EPA.For acidolysis reaction, all the lipases showed ability to incorporate n-3 PUFA into tuna and palm oils. C. antartica lipase had the maximum DHA incorporation into tuna and palm oils, rice bran lipase had relatively similar ability with R. miehei lipase, and the CPL lipase had the lowest ability. This study proved that rice bran and CPL lipases also had transesterification activity and showed the feasibility of the rice bran lipase to be a biocatalyst for n-3 PUFA-rich glyceride production. Increasing the substrate ratio, of n-3 PUFA concentrate and tuna or palm oil, could increase the EPA and DHA incorporation. The R. miehei, rice bran, and CPL lipases unabled to incorporate DHA into DHA-containing glyceride molecule, whereas C. antartica lipase had the capability in high ratio of n-3 PUFA concentrate to oil. Therefore, the lipases were easier to incorporate n-3 PUFA into palm oil than tuna oil, since the TAG molecules of palm oil was not as complex as tuna oil. It could be suggested that the lipases did not only have acyl chain and positional specificity, but also the whole glyceride structure specificity.Keywords : acidolysis, lipase, n-3 PUFA, palm oil, specificity, tuna oil. |
format |
Article in Journal/Newspaper |
author |
Elisabeth, Jenny Yuliani, T Tambunan, P M Purba, J M |
spellingShingle |
Elisabeth, Jenny Yuliani, T Tambunan, P M Purba, J M The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids |
author_facet |
Elisabeth, Jenny Yuliani, T Tambunan, P M Purba, J M |
author_sort |
Elisabeth, Jenny |
title |
The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids |
title_short |
The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids |
title_full |
The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids |
title_fullStr |
The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids |
title_full_unstemmed |
The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids |
title_sort |
specificity of several kinds lipases on n-3 polyunsaturated fatty acids |
publisher |
Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia |
publishDate |
2010 |
url |
http://journal.ipb.ac.id/index.php/jtip/article/view/820 |
genre |
antartic* |
genre_facet |
antartic* |
op_source |
Jurnal Teknologi dan Industri Pangan; Vol. 12 No. 1 (2001): Jurnal Teknologi dan Industri Pangan 2087-751X 1979-7788 |
op_relation |
http://journal.ipb.ac.id/index.php/jtip/article/view/820 |
_version_ |
1810291026080825344 |