VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TECHNIQUES TO MEASURE SERUM DEHYDROEPIANDROSTERONE (DHEA) AND DHEA-S IN NARWHALS (MONODON MONOCEROS)
Narwhals (Monodon monoceros) are increasingly exposed to anthropogenic disturbances that may increase their stress levels with unknown consequences for the overall population dynamics. The validation and measurement of chronic stress biomarkers could contribute toward improved understanding and cons...
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American Association of Zoo Veterinarians
2023
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ftbioone:10.1638/2022-0049 2023-07-30T04:02:29+02:00 VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TECHNIQUES TO MEASURE SERUM DEHYDROEPIANDROSTERONE (DHEA) AND DHEA-S IN NARWHALS (MONODON MONOCEROS) Karine Béland Stéphane Lair Mario Guay Tristan Juette Christian Bédard Sandra R. Black Marianne Marcoux Cortney A. Watt Nigel E. Hussey Marion Desmarchelier Karine Béland Stéphane Lair Mario Guay Tristan Juette Christian Bédard Sandra R. Black Marianne Marcoux Cortney A. Watt Nigel E. Hussey Marion Desmarchelier world 2023-03-20 text/HTML https://doi.org/10.1638/2022-0049 en eng American Association of Zoo Veterinarians doi:10.1638/2022-0049 All rights reserved. https://doi.org/10.1638/2022-0049 Text 2023 ftbioone https://doi.org/10.1638/2022-0049 2023-07-09T10:54:38Z Narwhals (Monodon monoceros) are increasingly exposed to anthropogenic disturbances that may increase their stress levels with unknown consequences for the overall population dynamics. The validation and measurement of chronic stress biomarkers could contribute toward improved understanding and conservation efforts for this species. Dehydroepiandrosterone (DHEA) and its sulfated metabolite DHEA-S are collectively referred to as DHEA(S). Serum DHEA(S) concentrations combined in ratios with cortisol [cortisol/DHEA(S)] have been shown to be promising indicators of chronic stress in humans, domestic animals, and wildlife. During field tagging in 2017 and 2018 in Baffin Bay, Nunavut, Canada, 14 wild narwhals were sampled at the beginning and end of the capture-tagging procedures. Serum DHEA(S) were measured with commercially available competitive enzyme-linked immunosorbent assays (ELISA) developed for humans. A partial validation of the ELISA assays was performed by the determination of the intra-assay coefficient of variation, confirmation of the DHEA(S) dilutional linearity, and the calculation of the percentage of recovery. Mean values (nanograms per milliliter ± standard error of the mean) of narwhal serum cortisol, DHEA(S), and cortisol/DHEA(S) ratios, at the beginning and at the end of handling, respectively, are reported (cortisol = 30.74 ± 4.87 and 41.83 ± 4.83; DHEA = 1.01 ± 0.52 and 0.99 ± 0.50; DHEA-S = 8.72 ± 1.68 and 7.70 ± 1.02; cortisol/DHEA = 75.43 ± 24.35 and 84.41 ± 11.76, and cortisol/DHEA-S = 4.16 ± 1.07 and 6.14 ± 1.00). Serum cortisol and cortisol/DHEA-S were statistically higher at the end of the capture (P= 0.024 and P= 0.035, respectively). Moreover, serum cortisol at the end of handling was positively correlated to total body length (P = 0.042) and tended to be higher in males (P = 0.086). These assays proved easy to perform, rapid, and suitable for measuring serum DHEA(S) of narwhals and that calculated cortisol/DHEA(S) are potential biomarkers for chronic stress in narwhals and possibly ... Text Baffin Bay Baffin Bay Baffin Monodon monoceros narwhal* Nunavut BioOne Online Journals Baffin Bay Canada Nunavut Journal of Zoo and Wildlife Medicine 54 1 |
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Open Polar |
collection |
BioOne Online Journals |
op_collection_id |
ftbioone |
language |
English |
description |
Narwhals (Monodon monoceros) are increasingly exposed to anthropogenic disturbances that may increase their stress levels with unknown consequences for the overall population dynamics. The validation and measurement of chronic stress biomarkers could contribute toward improved understanding and conservation efforts for this species. Dehydroepiandrosterone (DHEA) and its sulfated metabolite DHEA-S are collectively referred to as DHEA(S). Serum DHEA(S) concentrations combined in ratios with cortisol [cortisol/DHEA(S)] have been shown to be promising indicators of chronic stress in humans, domestic animals, and wildlife. During field tagging in 2017 and 2018 in Baffin Bay, Nunavut, Canada, 14 wild narwhals were sampled at the beginning and end of the capture-tagging procedures. Serum DHEA(S) were measured with commercially available competitive enzyme-linked immunosorbent assays (ELISA) developed for humans. A partial validation of the ELISA assays was performed by the determination of the intra-assay coefficient of variation, confirmation of the DHEA(S) dilutional linearity, and the calculation of the percentage of recovery. Mean values (nanograms per milliliter ± standard error of the mean) of narwhal serum cortisol, DHEA(S), and cortisol/DHEA(S) ratios, at the beginning and at the end of handling, respectively, are reported (cortisol = 30.74 ± 4.87 and 41.83 ± 4.83; DHEA = 1.01 ± 0.52 and 0.99 ± 0.50; DHEA-S = 8.72 ± 1.68 and 7.70 ± 1.02; cortisol/DHEA = 75.43 ± 24.35 and 84.41 ± 11.76, and cortisol/DHEA-S = 4.16 ± 1.07 and 6.14 ± 1.00). Serum cortisol and cortisol/DHEA-S were statistically higher at the end of the capture (P= 0.024 and P= 0.035, respectively). Moreover, serum cortisol at the end of handling was positively correlated to total body length (P = 0.042) and tended to be higher in males (P = 0.086). These assays proved easy to perform, rapid, and suitable for measuring serum DHEA(S) of narwhals and that calculated cortisol/DHEA(S) are potential biomarkers for chronic stress in narwhals and possibly ... |
author2 |
Karine Béland Stéphane Lair Mario Guay Tristan Juette Christian Bédard Sandra R. Black Marianne Marcoux Cortney A. Watt Nigel E. Hussey Marion Desmarchelier |
format |
Text |
author |
Karine Béland Stéphane Lair Mario Guay Tristan Juette Christian Bédard Sandra R. Black Marianne Marcoux Cortney A. Watt Nigel E. Hussey Marion Desmarchelier |
spellingShingle |
Karine Béland Stéphane Lair Mario Guay Tristan Juette Christian Bédard Sandra R. Black Marianne Marcoux Cortney A. Watt Nigel E. Hussey Marion Desmarchelier VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TECHNIQUES TO MEASURE SERUM DEHYDROEPIANDROSTERONE (DHEA) AND DHEA-S IN NARWHALS (MONODON MONOCEROS) |
author_facet |
Karine Béland Stéphane Lair Mario Guay Tristan Juette Christian Bédard Sandra R. Black Marianne Marcoux Cortney A. Watt Nigel E. Hussey Marion Desmarchelier |
author_sort |
Karine Béland |
title |
VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TECHNIQUES TO MEASURE SERUM DEHYDROEPIANDROSTERONE (DHEA) AND DHEA-S IN NARWHALS (MONODON MONOCEROS) |
title_short |
VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TECHNIQUES TO MEASURE SERUM DEHYDROEPIANDROSTERONE (DHEA) AND DHEA-S IN NARWHALS (MONODON MONOCEROS) |
title_full |
VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TECHNIQUES TO MEASURE SERUM DEHYDROEPIANDROSTERONE (DHEA) AND DHEA-S IN NARWHALS (MONODON MONOCEROS) |
title_fullStr |
VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TECHNIQUES TO MEASURE SERUM DEHYDROEPIANDROSTERONE (DHEA) AND DHEA-S IN NARWHALS (MONODON MONOCEROS) |
title_full_unstemmed |
VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TECHNIQUES TO MEASURE SERUM DEHYDROEPIANDROSTERONE (DHEA) AND DHEA-S IN NARWHALS (MONODON MONOCEROS) |
title_sort |
validation of enzyme-linked immunosorbent assay techniques to measure serum dehydroepiandrosterone (dhea) and dhea-s in narwhals (monodon monoceros) |
publisher |
American Association of Zoo Veterinarians |
publishDate |
2023 |
url |
https://doi.org/10.1638/2022-0049 |
op_coverage |
world |
geographic |
Baffin Bay Canada Nunavut |
geographic_facet |
Baffin Bay Canada Nunavut |
genre |
Baffin Bay Baffin Bay Baffin Monodon monoceros narwhal* Nunavut |
genre_facet |
Baffin Bay Baffin Bay Baffin Monodon monoceros narwhal* Nunavut |
op_source |
https://doi.org/10.1638/2022-0049 |
op_relation |
doi:10.1638/2022-0049 |
op_rights |
All rights reserved. |
op_doi |
https://doi.org/10.1638/2022-0049 |
container_title |
Journal of Zoo and Wildlife Medicine |
container_volume |
54 |
container_issue |
1 |
_version_ |
1772813301798928384 |