Time-Dependent Recovery of Mycoplasma lipofaciens (Strain ML64) from Incubated Infertile Chicken Eggs and Dead in Shell Chicken Embryos
Mycoplasmas are pathogens of different avian species, and they are able to be vertically transmitted. Even detected, Mycoplasma prevalence in raptor eggs is very low. In contrast to poultry, raptor eggs submitted for investigations are usually incubated. To investigate the influence of incubation le...
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American Association of Avian Pathologists
2008
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Online Access: | https://doi.org/10.1637/8200-122807-Reg.1 |
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ftbioone:10.1637/8200-122807-Reg.1 2023-07-30T03:55:23+02:00 Time-Dependent Recovery of Mycoplasma lipofaciens (Strain ML64) from Incubated Infertile Chicken Eggs and Dead in Shell Chicken Embryos Michael Lierz Hafez M. Hafez Michael Lierz Hafez M. Hafez world 2008-09-01 text/HTML https://doi.org/10.1637/8200-122807-Reg.1 en eng American Association of Avian Pathologists doi:10.1637/8200-122807-Reg.1 All rights reserved. https://doi.org/10.1637/8200-122807-Reg.1 Text 2008 ftbioone https://doi.org/10.1637/8200-122807-Reg.1 2023-07-09T10:39:55Z Mycoplasmas are pathogens of different avian species, and they are able to be vertically transmitted. Even detected, Mycoplasma prevalence in raptor eggs is very low. In contrast to poultry, raptor eggs submitted for investigations are usually incubated. To investigate the influence of incubation length on the recovery of mycoplasmas from eggs, infertile specific-pathogen-free chicken eggs and embryos were infected with Mycoplasma lipofaciens (strain ML64), which had previously been isolated from an egg of a northern goshawk (Accipiter gentilis), in two different dosages. The eggs were investigated up to 12 days after infection (infertile eggs) or embryonic death. Mycoplasmas were recovered over the entire period after embryonic death by isolation. It was possible to re-isolate M. lipofaciens (strain ML64) from infertile eggs infected with 106 colony-forming units (CFUs) up to 12 days, but only up to 7 days if infected with 102 CFUs, which may be closer to the situation after natural infection. This study demonstrates that incubation of infertile eggs does have an influence on the recovery rate of mycoplasmas. This influence must be considered if interpreting results of Mycoplasma investigations in eggs of nonpoultry species. Additionally, it is recommended to use dead in shell embryos rather than infertile eggs for Mycoplasma detection.Efecto del tiempo de incubación en el aislamiento de Mycoplasma lipofaciens (cepa ML64) a partir de huevos infértiles incubados y embriones de pollo muertos en el cascarón.Los micoplasmas son patógenos de diferentes especies aviares que pueden ser transmitidos verticalmente. Aun cuando se detectan, la prevalencia de micoplasmas en huevos de aves de presa es muy baja. Contrario a lo que ocurre en las aves domésticas, usualmente los huevos de aves de presa enviados para análisis han sido incubados. Para investigar la influencia de la duración de la incubación de los huevos sobre el aislamiento de Mycoplasma, se infectaron huevos infértiles y embriones de aves libres de patógenos ... Text Accipiter gentilis Northern Goshawk BioOne Online Journals Aun ENVELOPE(16.350,16.350,68.867,68.867) Baja Avian Diseases 52 3 441 443 |
institution |
Open Polar |
collection |
BioOne Online Journals |
op_collection_id |
ftbioone |
language |
English |
description |
Mycoplasmas are pathogens of different avian species, and they are able to be vertically transmitted. Even detected, Mycoplasma prevalence in raptor eggs is very low. In contrast to poultry, raptor eggs submitted for investigations are usually incubated. To investigate the influence of incubation length on the recovery of mycoplasmas from eggs, infertile specific-pathogen-free chicken eggs and embryos were infected with Mycoplasma lipofaciens (strain ML64), which had previously been isolated from an egg of a northern goshawk (Accipiter gentilis), in two different dosages. The eggs were investigated up to 12 days after infection (infertile eggs) or embryonic death. Mycoplasmas were recovered over the entire period after embryonic death by isolation. It was possible to re-isolate M. lipofaciens (strain ML64) from infertile eggs infected with 106 colony-forming units (CFUs) up to 12 days, but only up to 7 days if infected with 102 CFUs, which may be closer to the situation after natural infection. This study demonstrates that incubation of infertile eggs does have an influence on the recovery rate of mycoplasmas. This influence must be considered if interpreting results of Mycoplasma investigations in eggs of nonpoultry species. Additionally, it is recommended to use dead in shell embryos rather than infertile eggs for Mycoplasma detection.Efecto del tiempo de incubación en el aislamiento de Mycoplasma lipofaciens (cepa ML64) a partir de huevos infértiles incubados y embriones de pollo muertos en el cascarón.Los micoplasmas son patógenos de diferentes especies aviares que pueden ser transmitidos verticalmente. Aun cuando se detectan, la prevalencia de micoplasmas en huevos de aves de presa es muy baja. Contrario a lo que ocurre en las aves domésticas, usualmente los huevos de aves de presa enviados para análisis han sido incubados. Para investigar la influencia de la duración de la incubación de los huevos sobre el aislamiento de Mycoplasma, se infectaron huevos infértiles y embriones de aves libres de patógenos ... |
author2 |
Michael Lierz Hafez M. Hafez |
format |
Text |
author |
Michael Lierz Hafez M. Hafez |
spellingShingle |
Michael Lierz Hafez M. Hafez Time-Dependent Recovery of Mycoplasma lipofaciens (Strain ML64) from Incubated Infertile Chicken Eggs and Dead in Shell Chicken Embryos |
author_facet |
Michael Lierz Hafez M. Hafez |
author_sort |
Michael Lierz |
title |
Time-Dependent Recovery of Mycoplasma lipofaciens (Strain ML64) from Incubated Infertile Chicken Eggs and Dead in Shell Chicken Embryos |
title_short |
Time-Dependent Recovery of Mycoplasma lipofaciens (Strain ML64) from Incubated Infertile Chicken Eggs and Dead in Shell Chicken Embryos |
title_full |
Time-Dependent Recovery of Mycoplasma lipofaciens (Strain ML64) from Incubated Infertile Chicken Eggs and Dead in Shell Chicken Embryos |
title_fullStr |
Time-Dependent Recovery of Mycoplasma lipofaciens (Strain ML64) from Incubated Infertile Chicken Eggs and Dead in Shell Chicken Embryos |
title_full_unstemmed |
Time-Dependent Recovery of Mycoplasma lipofaciens (Strain ML64) from Incubated Infertile Chicken Eggs and Dead in Shell Chicken Embryos |
title_sort |
time-dependent recovery of mycoplasma lipofaciens (strain ml64) from incubated infertile chicken eggs and dead in shell chicken embryos |
publisher |
American Association of Avian Pathologists |
publishDate |
2008 |
url |
https://doi.org/10.1637/8200-122807-Reg.1 |
op_coverage |
world |
long_lat |
ENVELOPE(16.350,16.350,68.867,68.867) |
geographic |
Aun Baja |
geographic_facet |
Aun Baja |
genre |
Accipiter gentilis Northern Goshawk |
genre_facet |
Accipiter gentilis Northern Goshawk |
op_source |
https://doi.org/10.1637/8200-122807-Reg.1 |
op_relation |
doi:10.1637/8200-122807-Reg.1 |
op_rights |
All rights reserved. |
op_doi |
https://doi.org/10.1637/8200-122807-Reg.1 |
container_title |
Avian Diseases |
container_volume |
52 |
container_issue |
3 |
container_start_page |
441 |
op_container_end_page |
443 |
_version_ |
1772809738019405824 |