Development of a Highly Sensitive Screen for Influenza A in Guano and Its Application in the Search for Ancient RNA Preserved Under Antarctic Adelie Penguin Colonies

• Published in: Avian Diseases
• Main Authors: L. C. Briggs, R. M. Ashton, P. Metcalf
• Format: Text
• Language: English
• Published: American Association of Avian Pathologists 2003
• Subjects: Antarctic; Guano; Adelie penguin; Antarc*; Pygoscelis adeliae
• Online Access: https://doi.org/10.1637/0005-2086-47.s3.1200

We have developed a reverse transcriptase polymerase chain reaction (RT-PCR)-based assay to detect influenza A in guano samples as part of our program to investigate ancient viral RNA from under Antarctic Adelie penguin (Pygoscelis adeliae) colonies. Of five extraction protocols tested (RNeasy, GTC...