An Improved Extraction Method to Increase DNA Yield from Molted Feathers
To assess the value of molted feathers as a noninvasive source of DNA for genetic studies of Northern Goshawks (Accipiter gentilis), we isolated and quantified DNA from molted feathers and compared yields across five feather types. We also compared PCR success across the same five feather types usin...
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American Ornithological Society
2008
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ftbioone:10.1525/cond.2008.8586 2024-05-12T07:52:04+00:00 An Improved Extraction Method to Increase DNA Yield from Molted Feathers Shelley Bayard De Volo Richard T. Reynolds Marlis R. Douglas Michael F. Antolin Shelley Bayard De Volo Richard T. Reynolds Marlis R. Douglas Michael F. Antolin world 2008-11-01 text/HTML https://doi.org/10.1525/cond.2008.8586 en eng American Ornithological Society doi:10.1525/cond.2008.8586 All rights reserved. https://doi.org/10.1525/cond.2008.8586 Text 2008 ftbioone https://doi.org/10.1525/cond.2008.8586 2024-04-16T02:14:21Z To assess the value of molted feathers as a noninvasive source of DNA for genetic studies of Northern Goshawks (Accipiter gentilis), we isolated and quantified DNA from molted feathers and compared yields across five feather types. We also compared PCR success across the same five feather types using five microsatellite genetic markers of varying size. In addition, we compared DNA yields from a commonly used extraction method versus one we modified to increase DNA yield. Results indicated molted feathers provided on average 24 ng μl−1 of DNA, which is a relatively high DNA yield compared to other noninvasive tissue sources. Tail feathers yielded significantly more DNA than primary, secondary, and smaller feathers, yet all feather sizes produced equally high rates of PCR success. Although our modified extraction method increased the time required for processing feathers, it resulted in significantly higher yields of DNA as compared to the unmodified protocol. Text Accipiter gentilis BioOne Online Journals The Condor 110 4 762 766 |
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BioOne Online Journals |
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English |
description |
To assess the value of molted feathers as a noninvasive source of DNA for genetic studies of Northern Goshawks (Accipiter gentilis), we isolated and quantified DNA from molted feathers and compared yields across five feather types. We also compared PCR success across the same five feather types using five microsatellite genetic markers of varying size. In addition, we compared DNA yields from a commonly used extraction method versus one we modified to increase DNA yield. Results indicated molted feathers provided on average 24 ng μl−1 of DNA, which is a relatively high DNA yield compared to other noninvasive tissue sources. Tail feathers yielded significantly more DNA than primary, secondary, and smaller feathers, yet all feather sizes produced equally high rates of PCR success. Although our modified extraction method increased the time required for processing feathers, it resulted in significantly higher yields of DNA as compared to the unmodified protocol. |
author2 |
Shelley Bayard De Volo Richard T. Reynolds Marlis R. Douglas Michael F. Antolin |
format |
Text |
author |
Shelley Bayard De Volo Richard T. Reynolds Marlis R. Douglas Michael F. Antolin |
spellingShingle |
Shelley Bayard De Volo Richard T. Reynolds Marlis R. Douglas Michael F. Antolin An Improved Extraction Method to Increase DNA Yield from Molted Feathers |
author_facet |
Shelley Bayard De Volo Richard T. Reynolds Marlis R. Douglas Michael F. Antolin |
author_sort |
Shelley Bayard De Volo |
title |
An Improved Extraction Method to Increase DNA Yield from Molted Feathers |
title_short |
An Improved Extraction Method to Increase DNA Yield from Molted Feathers |
title_full |
An Improved Extraction Method to Increase DNA Yield from Molted Feathers |
title_fullStr |
An Improved Extraction Method to Increase DNA Yield from Molted Feathers |
title_full_unstemmed |
An Improved Extraction Method to Increase DNA Yield from Molted Feathers |
title_sort |
improved extraction method to increase dna yield from molted feathers |
publisher |
American Ornithological Society |
publishDate |
2008 |
url |
https://doi.org/10.1525/cond.2008.8586 |
op_coverage |
world |
genre |
Accipiter gentilis |
genre_facet |
Accipiter gentilis |
op_source |
https://doi.org/10.1525/cond.2008.8586 |
op_relation |
doi:10.1525/cond.2008.8586 |
op_rights |
All rights reserved. |
op_doi |
https://doi.org/10.1525/cond.2008.8586 |
container_title |
The Condor |
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110 |
container_issue |
4 |
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762 |
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766 |
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1798835715413377024 |