Unexpected Foraminiferal Diversity Revealed by Small-subunit rDNA Analysis of Antarctic Sediment
Studies of benthic Foraminifera typically rely on the morphological identification of dried specimens. This approach can introduce sampling bias against small, delicate, or morphologically ambiguous forms. To overcome this limitation, we extracted total DNA from sediment followed by PCR using group-...
Published in: | The Journal of Eukaryotic Microbiology |
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International Society of Protistologists
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ftbioone:10.1111/j.1550-7408.2004.tb00542.x 2024-06-02T07:56:38+00:00 Unexpected Foraminiferal Diversity Revealed by Small-subunit rDNA Analysis of Antarctic Sediment ANDREA HABURA JAN PAWLOWSKI STEVEN D. HANES SAMUEL S. BOWSER ANDREA HABURA JAN PAWLOWSKI STEVEN D. HANES SAMUEL S. BOWSER world 2004-03-01 text/HTML https://doi.org/10.1111/j.1550-7408.2004.tb00542.x en eng International Society of Protistologists doi:10.1111/j.1550-7408.2004.tb00542.x All rights reserved. https://doi.org/10.1111/j.1550-7408.2004.tb00542.x Text 2004 ftbioone https://doi.org/10.1111/j.1550-7408.2004.tb00542.x 2024-05-07T00:59:41Z Studies of benthic Foraminifera typically rely on the morphological identification of dried specimens. This approach can introduce sampling bias against small, delicate, or morphologically ambiguous forms. To overcome this limitation, we extracted total DNA from sediment followed by PCR using group- and species-specific primers. Phylogenetic analyses revealed that approximately ninety percent of the PCR products represented previously undescribed sequence types that group with undersampled members of the allogromiid Foraminifera. We also used a modification of this technique to track individual species in sediment fractions too fine for normal morphological identification, and to confirm species placement of morphologically ambiguous foraminiferans. We were able to identify the DNA of several large foraminiferal species in fine fractions in a seasonally-dependent manner, indicating that in some seasons the majority of the standing stock of these species exists as gametes/juveniles. The approach outlined here represents a powerful strategy for exploring the total diversity of benthic foraminiferal communities. Text Antarc* Antarctic BioOne Online Journals Antarctic The Journal of Eukaryotic Microbiology 51 2 173 179 |
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Open Polar |
collection |
BioOne Online Journals |
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ftbioone |
language |
English |
description |
Studies of benthic Foraminifera typically rely on the morphological identification of dried specimens. This approach can introduce sampling bias against small, delicate, or morphologically ambiguous forms. To overcome this limitation, we extracted total DNA from sediment followed by PCR using group- and species-specific primers. Phylogenetic analyses revealed that approximately ninety percent of the PCR products represented previously undescribed sequence types that group with undersampled members of the allogromiid Foraminifera. We also used a modification of this technique to track individual species in sediment fractions too fine for normal morphological identification, and to confirm species placement of morphologically ambiguous foraminiferans. We were able to identify the DNA of several large foraminiferal species in fine fractions in a seasonally-dependent manner, indicating that in some seasons the majority of the standing stock of these species exists as gametes/juveniles. The approach outlined here represents a powerful strategy for exploring the total diversity of benthic foraminiferal communities. |
author2 |
ANDREA HABURA JAN PAWLOWSKI STEVEN D. HANES SAMUEL S. BOWSER |
format |
Text |
author |
ANDREA HABURA JAN PAWLOWSKI STEVEN D. HANES SAMUEL S. BOWSER |
spellingShingle |
ANDREA HABURA JAN PAWLOWSKI STEVEN D. HANES SAMUEL S. BOWSER Unexpected Foraminiferal Diversity Revealed by Small-subunit rDNA Analysis of Antarctic Sediment |
author_facet |
ANDREA HABURA JAN PAWLOWSKI STEVEN D. HANES SAMUEL S. BOWSER |
author_sort |
ANDREA HABURA |
title |
Unexpected Foraminiferal Diversity Revealed by Small-subunit rDNA Analysis of Antarctic Sediment |
title_short |
Unexpected Foraminiferal Diversity Revealed by Small-subunit rDNA Analysis of Antarctic Sediment |
title_full |
Unexpected Foraminiferal Diversity Revealed by Small-subunit rDNA Analysis of Antarctic Sediment |
title_fullStr |
Unexpected Foraminiferal Diversity Revealed by Small-subunit rDNA Analysis of Antarctic Sediment |
title_full_unstemmed |
Unexpected Foraminiferal Diversity Revealed by Small-subunit rDNA Analysis of Antarctic Sediment |
title_sort |
unexpected foraminiferal diversity revealed by small-subunit rdna analysis of antarctic sediment |
publisher |
International Society of Protistologists |
publishDate |
2004 |
url |
https://doi.org/10.1111/j.1550-7408.2004.tb00542.x |
op_coverage |
world |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
https://doi.org/10.1111/j.1550-7408.2004.tb00542.x |
op_relation |
doi:10.1111/j.1550-7408.2004.tb00542.x |
op_rights |
All rights reserved. |
op_doi |
https://doi.org/10.1111/j.1550-7408.2004.tb00542.x |
container_title |
The Journal of Eukaryotic Microbiology |
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51 |
container_issue |
2 |
container_start_page |
173 |
op_container_end_page |
179 |
_version_ |
1800757478630096896 |