Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previou...
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Society for the Study of Reproduction
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ftbioone:10.1095/biolreprod.113.109728 2024-06-02T08:05:38+00:00 Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1 Eric Guévélou Arnaud Huvet Clara E. Galindo-Sánchez Massimo Milan Virgile Quillien Jean-Yves Daniel Claudie Quéré Pierre Boudry Charlotte Corporeau Eric Guévélou Arnaud Huvet Clara E. Galindo-Sánchez Massimo Milan Virgile Quillien Jean-Yves Daniel Claudie Quéré Pierre Boudry Charlotte Corporeau world 2013-08-07 text/HTML https://doi.org/10.1095/biolreprod.113.109728 en eng Society for the Study of Reproduction doi:10.1095/biolreprod.113.109728 All rights reserved. https://doi.org/10.1095/biolreprod.113.109728 Text 2013 ftbioone https://doi.org/10.1095/biolreprod.113.109728 2024-05-07T00:58:23Z The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. Text Crassostrea gigas Pacific oyster BioOne Online Journals Pacific Biology of Reproduction 89 4 |
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Open Polar |
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BioOne Online Journals |
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ftbioone |
language |
English |
description |
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. |
author2 |
Eric Guévélou Arnaud Huvet Clara E. Galindo-Sánchez Massimo Milan Virgile Quillien Jean-Yves Daniel Claudie Quéré Pierre Boudry Charlotte Corporeau |
format |
Text |
author |
Eric Guévélou Arnaud Huvet Clara E. Galindo-Sánchez Massimo Milan Virgile Quillien Jean-Yves Daniel Claudie Quéré Pierre Boudry Charlotte Corporeau |
spellingShingle |
Eric Guévélou Arnaud Huvet Clara E. Galindo-Sánchez Massimo Milan Virgile Quillien Jean-Yves Daniel Claudie Quéré Pierre Boudry Charlotte Corporeau Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1 |
author_facet |
Eric Guévélou Arnaud Huvet Clara E. Galindo-Sánchez Massimo Milan Virgile Quillien Jean-Yves Daniel Claudie Quéré Pierre Boudry Charlotte Corporeau |
author_sort |
Eric Guévélou |
title |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1 |
title_short |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1 |
title_full |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1 |
title_fullStr |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1 |
title_full_unstemmed |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1 |
title_sort |
sex-specific regulation of amp-activated protein kinase (ampk) in the pacific oyster crassostrea gigas1 |
publisher |
Society for the Study of Reproduction |
publishDate |
2013 |
url |
https://doi.org/10.1095/biolreprod.113.109728 |
op_coverage |
world |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
https://doi.org/10.1095/biolreprod.113.109728 |
op_relation |
doi:10.1095/biolreprod.113.109728 |
op_rights |
All rights reserved. |
op_doi |
https://doi.org/10.1095/biolreprod.113.109728 |
container_title |
Biology of Reproduction |
container_volume |
89 |
container_issue |
4 |
_version_ |
1800750495380275200 |