Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1
Research was conducted to define the basic reproductive physiology of killer whales (Orcinus orca) and to use this knowledge to facilitate the development of artificial insemination procedures. The specific objectives were 1) to determine the excretory dynamics of urinary LH and ovarian steroid meta...
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ftbioone:10.1095/biolreprod.104.027961 2024-06-02T08:12:48+00:00 Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1 T. R. Robeck K. J. Steinman S. Gearhart T. R. Reidarson J. F. McBain S. L. Monfort T. R. Robeck K. J. Steinman S. Gearhart T. R. Reidarson J. F. McBain S. L. Monfort world 2004-08-01 text/HTML https://doi.org/10.1095/biolreprod.104.027961 en eng Society for the Study of Reproduction doi:10.1095/biolreprod.104.027961 All rights reserved. https://doi.org/10.1095/biolreprod.104.027961 Text 2004 ftbioone https://doi.org/10.1095/biolreprod.104.027961 2024-05-07T00:57:06Z Research was conducted to define the basic reproductive physiology of killer whales (Orcinus orca) and to use this knowledge to facilitate the development of artificial insemination procedures. The specific objectives were 1) to determine the excretory dynamics of urinary LH and ovarian steroid metabolites during the estrous cycle; 2) to evaluate the effect of an exogenously administered, synthetic progesterone analog on reproductive hormone excretion; 3) to validate the use of transabdominal ultrasound for ovarian evaluation and timing of ovulation; 4) to examine the quality of semen after liquid storage and cryopreservation; and 5) to develop an intrauterine insemination technique. Based on urinary endocrine monitoring of 41 follicular phases and 26 complete cycles from five females, estrous cycles were 41 days long and comprised a 17-day follicular phase and a 21-day luteal phase. A consistent temporal relationship was observed between peak estrogen conjugates and the LH surge, the latter of which occurred approximately 0.5 days later. Two animals placed on oral altrenogest (three separate occasions for 30, 17, and 31 days, respectively) excreted peak urinary estrogen concentrations 25 days after withdrawal that were followed by sustained elevations in urinary pregnanediol-3α-glucuronide excretion. Mean preovulatory follicle diameter was 3.9 cm (n = 6), and ovulation occurred 38 h (n = 5) after the peak of the LH surge. Based on visual estimates of motility, liquid-stored semen maintained 92% of its raw ejaculate sperm motility index (total progressive motility × kinetic rating [0–5 scale, where 0 = no movement and 5 = rapid progressive movement]) when held at 4°C for 3 days postcollection. Semen cryopreserved using a medium freezing rate demonstrated good postthaw total motility (50%), progressive motility (94%), and kinetic rating (3.5). Insemination during eight estrous cycles resulted in three pregnancies (38%), two from liquid-stored and one from cryopreserved semen. Two calves were delivered after ... Text Orca Orcinus orca BioOne Online Journals Biology of Reproduction 71 2 650 660 |
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English |
description |
Research was conducted to define the basic reproductive physiology of killer whales (Orcinus orca) and to use this knowledge to facilitate the development of artificial insemination procedures. The specific objectives were 1) to determine the excretory dynamics of urinary LH and ovarian steroid metabolites during the estrous cycle; 2) to evaluate the effect of an exogenously administered, synthetic progesterone analog on reproductive hormone excretion; 3) to validate the use of transabdominal ultrasound for ovarian evaluation and timing of ovulation; 4) to examine the quality of semen after liquid storage and cryopreservation; and 5) to develop an intrauterine insemination technique. Based on urinary endocrine monitoring of 41 follicular phases and 26 complete cycles from five females, estrous cycles were 41 days long and comprised a 17-day follicular phase and a 21-day luteal phase. A consistent temporal relationship was observed between peak estrogen conjugates and the LH surge, the latter of which occurred approximately 0.5 days later. Two animals placed on oral altrenogest (three separate occasions for 30, 17, and 31 days, respectively) excreted peak urinary estrogen concentrations 25 days after withdrawal that were followed by sustained elevations in urinary pregnanediol-3α-glucuronide excretion. Mean preovulatory follicle diameter was 3.9 cm (n = 6), and ovulation occurred 38 h (n = 5) after the peak of the LH surge. Based on visual estimates of motility, liquid-stored semen maintained 92% of its raw ejaculate sperm motility index (total progressive motility × kinetic rating [0–5 scale, where 0 = no movement and 5 = rapid progressive movement]) when held at 4°C for 3 days postcollection. Semen cryopreserved using a medium freezing rate demonstrated good postthaw total motility (50%), progressive motility (94%), and kinetic rating (3.5). Insemination during eight estrous cycles resulted in three pregnancies (38%), two from liquid-stored and one from cryopreserved semen. Two calves were delivered after ... |
author2 |
T. R. Robeck K. J. Steinman S. Gearhart T. R. Reidarson J. F. McBain S. L. Monfort |
format |
Text |
author |
T. R. Robeck K. J. Steinman S. Gearhart T. R. Reidarson J. F. McBain S. L. Monfort |
spellingShingle |
T. R. Robeck K. J. Steinman S. Gearhart T. R. Reidarson J. F. McBain S. L. Monfort Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1 |
author_facet |
T. R. Robeck K. J. Steinman S. Gearhart T. R. Reidarson J. F. McBain S. L. Monfort |
author_sort |
T. R. Robeck |
title |
Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1 |
title_short |
Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1 |
title_full |
Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1 |
title_fullStr |
Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1 |
title_full_unstemmed |
Reproductive Physiology and Development of Artificial Insemination Technology in Killer Whales (Orcinus orca)1 |
title_sort |
reproductive physiology and development of artificial insemination technology in killer whales (orcinus orca)1 |
publisher |
Society for the Study of Reproduction |
publishDate |
2004 |
url |
https://doi.org/10.1095/biolreprod.104.027961 |
op_coverage |
world |
genre |
Orca Orcinus orca |
genre_facet |
Orca Orcinus orca |
op_source |
https://doi.org/10.1095/biolreprod.104.027961 |
op_relation |
doi:10.1095/biolreprod.104.027961 |
op_rights |
All rights reserved. |
op_doi |
https://doi.org/10.1095/biolreprod.104.027961 |
container_title |
Biology of Reproduction |
container_volume |
71 |
container_issue |
2 |
container_start_page |
650 |
op_container_end_page |
660 |
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1800759352225693696 |