DNA persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses
Molecular forensics is an important component of wildlife research and management. Using DNA from noninvasive samples collected at predation sites, we can identify predator species and obtain individual genotypes, improving our understanding of predator–prey dynamics and impacts of predators on live...
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American Society of Mammalogists
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Online Access: | https://doi.org/10.1093/jmammal/gyz156 |
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ftbioone:10.1093/jmammal/gyz156 2024-06-02T08:05:03+00:00 DNA persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses Antoinette J. Piaggio Susan A. Shriner Julie K. Young Doreen L. Griffin Peggy Callahan Darren J. Wostenberg Eric M. Gese Matthew W. Hopken Antoinette J. Piaggio Susan A. Shriner Julie K. Young Doreen L. Griffin Peggy Callahan Darren J. Wostenberg Eric M. Gese Matthew W. Hopken world 2019-11-20 text/HTML https://doi.org/10.1093/jmammal/gyz156 en eng American Society of Mammalogists doi:10.1093/jmammal/gyz156 All rights reserved. https://doi.org/10.1093/jmammal/gyz156 Text 2019 ftbioone https://doi.org/10.1093/jmammal/gyz156 2024-05-07T00:55:29Z Molecular forensics is an important component of wildlife research and management. Using DNA from noninvasive samples collected at predation sites, we can identify predator species and obtain individual genotypes, improving our understanding of predator–prey dynamics and impacts of predators on livestock and endangered species. To improve sample collection strategies, we tested two sample collection methods and estimated degradation rates of predator DNA on the carcasses of multiple prey species. We fed carcasses of calves (Bos taurus) and lambs (Ovis aires) to three captive predator species: wolves (Canis lupus), coyotes (C. latrans), and mountain lions (Puma concolor). We swabbed the carcass in the field, as well as removed a piece of hide from the carcasses and then swabbed it in the laboratory. We swabbed all tissue samples through time and attempted to identify the predator involved in the depredation using salivary DNA. We found the most successful approach for yielding viable salivary DNA was removing hide from the prey and swabbing it in the laboratory. As expected, genotyping error increased through time and our ability to obtain complete genotypes decreased over time, the latter falling below 50% after 24 h. We provide guidelines for sampling salivary DNA from tissues of depredated carcasses for maximum probability of detection. Text Canis lupus BioOne Online Journals Journal of Mammalogy |
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Molecular forensics is an important component of wildlife research and management. Using DNA from noninvasive samples collected at predation sites, we can identify predator species and obtain individual genotypes, improving our understanding of predator–prey dynamics and impacts of predators on livestock and endangered species. To improve sample collection strategies, we tested two sample collection methods and estimated degradation rates of predator DNA on the carcasses of multiple prey species. We fed carcasses of calves (Bos taurus) and lambs (Ovis aires) to three captive predator species: wolves (Canis lupus), coyotes (C. latrans), and mountain lions (Puma concolor). We swabbed the carcass in the field, as well as removed a piece of hide from the carcasses and then swabbed it in the laboratory. We swabbed all tissue samples through time and attempted to identify the predator involved in the depredation using salivary DNA. We found the most successful approach for yielding viable salivary DNA was removing hide from the prey and swabbing it in the laboratory. As expected, genotyping error increased through time and our ability to obtain complete genotypes decreased over time, the latter falling below 50% after 24 h. We provide guidelines for sampling salivary DNA from tissues of depredated carcasses for maximum probability of detection. |
author2 |
Antoinette J. Piaggio Susan A. Shriner Julie K. Young Doreen L. Griffin Peggy Callahan Darren J. Wostenberg Eric M. Gese Matthew W. Hopken |
format |
Text |
author |
Antoinette J. Piaggio Susan A. Shriner Julie K. Young Doreen L. Griffin Peggy Callahan Darren J. Wostenberg Eric M. Gese Matthew W. Hopken |
spellingShingle |
Antoinette J. Piaggio Susan A. Shriner Julie K. Young Doreen L. Griffin Peggy Callahan Darren J. Wostenberg Eric M. Gese Matthew W. Hopken DNA persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses |
author_facet |
Antoinette J. Piaggio Susan A. Shriner Julie K. Young Doreen L. Griffin Peggy Callahan Darren J. Wostenberg Eric M. Gese Matthew W. Hopken |
author_sort |
Antoinette J. Piaggio |
title |
DNA persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses |
title_short |
DNA persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses |
title_full |
DNA persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses |
title_fullStr |
DNA persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses |
title_full_unstemmed |
DNA persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses |
title_sort |
dna persistence in predator saliva from multiple species and methods for optimal recovery from depredated carcasses |
publisher |
American Society of Mammalogists |
publishDate |
2019 |
url |
https://doi.org/10.1093/jmammal/gyz156 |
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world |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
https://doi.org/10.1093/jmammal/gyz156 |
op_relation |
doi:10.1093/jmammal/gyz156 |
op_rights |
All rights reserved. |
op_doi |
https://doi.org/10.1093/jmammal/gyz156 |
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Journal of Mammalogy |
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1800749788264660992 |