Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla
In a study of reproduction in male European eels Anguilla anguilla, we induced spermatogenesis through hormone injection and established a spermatogenic maturity index (SMI) as a novel quantification of testis development. Eels in the experiments were sacrificed weekly and testis tissue was sampled...
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American Fisheries Society
2011
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Online Access: | https://doi.org/10.1080/19425120.2011.556902 |
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ftbioone:10.1080/19425120.2011.556902 2024-06-02T07:55:10+00:00 Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla Jonna Tomkiewicz Tanja M. N. Kofoed Jonna Tomkiewicz Tanja M. N. Kofoed world 2011-01-01 text/HTML https://doi.org/10.1080/19425120.2011.556902 en eng American Fisheries Society doi:10.1080/19425120.2011.556902 All rights reserved. https://doi.org/10.1080/19425120.2011.556902 Text 2011 ftbioone https://doi.org/10.1080/19425120.2011.556902 2024-05-07T00:51:43Z In a study of reproduction in male European eels Anguilla anguilla, we induced spermatogenesis through hormone injection and established a spermatogenic maturity index (SMI) as a novel quantification of testis development. Eels in the experiments were sacrificed weekly and testis tissue was sampled for histological analysis of spermatogenesis. Testis development was followed over 18 weeks, during which the males continued to develop spermatocytes and produce spermatozoa. The SMI describes testis development from estimation of the area fractions of various tissue categories characterized by progressive gamete development stages in histological sections of the testes. The index weighs the volume fractions of the different tissues (somatic cells and germ cell stages) and describes development on a scale of 0 to 1. The method improves the existing histological classification, providing a quantitative measure that reflects the spermatogenic process and can be correlated with morphological and physiological parameters. In this study, the SMI reacted immediately to the onset of spermatogenesis and increased linearly over time, tracking the development of spermatocysts and spermatozoa. In week 7, the SMI reached a stable level of around 0.75, where it remained, with limited fluctuations, until the end of the experiment. This reflected the composition of different germ cell stages in the testis tissue with a continuous generation of spermatocysts and production of spermatozoa. In comparison, the gonadosomatic index showed a delayed response to the onset of spermatogenesis and fluctuated substantially during the sperm production period. The properties of the SMI made it a useful index for describing spermatogenesis in male European eels during this experiment and a promising tool for quantifying testis development and describing male reproductive strategy in other fish species. Text Anguilla anguilla European eel BioOne Online Journals Marine and Coastal Fisheries 3 1 106 118 |
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description |
In a study of reproduction in male European eels Anguilla anguilla, we induced spermatogenesis through hormone injection and established a spermatogenic maturity index (SMI) as a novel quantification of testis development. Eels in the experiments were sacrificed weekly and testis tissue was sampled for histological analysis of spermatogenesis. Testis development was followed over 18 weeks, during which the males continued to develop spermatocytes and produce spermatozoa. The SMI describes testis development from estimation of the area fractions of various tissue categories characterized by progressive gamete development stages in histological sections of the testes. The index weighs the volume fractions of the different tissues (somatic cells and germ cell stages) and describes development on a scale of 0 to 1. The method improves the existing histological classification, providing a quantitative measure that reflects the spermatogenic process and can be correlated with morphological and physiological parameters. In this study, the SMI reacted immediately to the onset of spermatogenesis and increased linearly over time, tracking the development of spermatocysts and spermatozoa. In week 7, the SMI reached a stable level of around 0.75, where it remained, with limited fluctuations, until the end of the experiment. This reflected the composition of different germ cell stages in the testis tissue with a continuous generation of spermatocysts and production of spermatozoa. In comparison, the gonadosomatic index showed a delayed response to the onset of spermatogenesis and fluctuated substantially during the sperm production period. The properties of the SMI made it a useful index for describing spermatogenesis in male European eels during this experiment and a promising tool for quantifying testis development and describing male reproductive strategy in other fish species. |
author2 |
Jonna Tomkiewicz Tanja M. N. Kofoed |
format |
Text |
author |
Jonna Tomkiewicz Tanja M. N. Kofoed |
spellingShingle |
Jonna Tomkiewicz Tanja M. N. Kofoed Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
author_facet |
Jonna Tomkiewicz Tanja M. N. Kofoed |
author_sort |
Jonna Tomkiewicz |
title |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_short |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_full |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_fullStr |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_full_unstemmed |
Assessment of Testis Development during Induced Spermatogenesis in the European Eel Anguilla anguilla |
title_sort |
assessment of testis development during induced spermatogenesis in the european eel anguilla anguilla |
publisher |
American Fisheries Society |
publishDate |
2011 |
url |
https://doi.org/10.1080/19425120.2011.556902 |
op_coverage |
world |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
https://doi.org/10.1080/19425120.2011.556902 |
op_relation |
doi:10.1080/19425120.2011.556902 |
op_rights |
All rights reserved. |
op_doi |
https://doi.org/10.1080/19425120.2011.556902 |
container_title |
Marine and Coastal Fisheries |
container_volume |
3 |
container_issue |
1 |
container_start_page |
106 |
op_container_end_page |
118 |
_version_ |
1800746457061392384 |