Complete genome sequence of Lutibacter profundi LP1 T isolated from an Arctic deep-sea hydrothermal vent system
Abstract Lutibacter profundi LP1 T within the family Flavobacteriaceae was isolated from a biofilm growing on the surface of a black smoker chimney at the Loki’s Castle vent field, located on the Arctic Mid-Ocean Ridge. The complete genome of L. profundi LP1 T is the first genome to be published wit...
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ftbiomed:oai:biomedcentral.com:s40793-016-0219-x 2023-05-15T15:03:51+02:00 Complete genome sequence of Lutibacter profundi LP1 T isolated from an Arctic deep-sea hydrothermal vent system Wissuwa, Juliane Bauer, Sven Steen, Ida Stokke, Runar 2017-01-07 http://www.standardsingenomics.com/content/12/1/5 en eng BioMed Central Ltd. http://www.standardsingenomics.com/content/12/1/5 Copyright 2017 The Author(s). Lutibacter Flavobacteriaceae Loki’s castle Biofilm Deep-sea hydrothermal vent Short genome report 2017 ftbiomed 2017-01-15T00:56:55Z Abstract Lutibacter profundi LP1 T within the family Flavobacteriaceae was isolated from a biofilm growing on the surface of a black smoker chimney at the Loki’s Castle vent field, located on the Arctic Mid-Ocean Ridge. The complete genome of L. profundi LP1 T is the first genome to be published within the genus Lutibacter. L. profundi LP1 T consists of a single 2,966,978 bp circular chromosome with a GC content of 29.8%. The genome comprises 2,537 protein-coding genes, 40 tRNA species and 2 rRNA operons. The microaerophilic, organotrophic isolate contains genes for all central carbohydrate metabolic pathways. However, genes for the oxidative branch of the pentose-phosphate-pathway, the glyoxylate shunt of the tricarboxylic acid cycle and the ATP citrate lyase for reverse TCA are not present. L. profundi LP1 T utilizes starch, sucrose and diverse proteinous carbon sources. In accordance, the genome harbours 130 proteases and 104 carbohydrate-active enzymes, indicating a specialization in degrading organic matter. Among a small arsenal of 24 glycosyl hydrolases, which offer the possibility to hydrolyse diverse poly- and oligosaccharides, a starch utilization cluster was identified. Furthermore, a variety of enzymes may be secreted via T9SS and contribute to the hydrolytic variety of the microorganism. Genes for gliding motility are present, which may enable the bacteria to move within the biofilm. A substantial number of genes encoding for extracellular polysaccharide synthesis pathways, curli fibres and attachment to surfaces could mediate adhesion in the biofilm and may contribute to the biofilm formation. In addition to aerobic respiration, the complete denitrification pathway and genes for sulphide oxidation e.g. sulphide:quinone reductase are present in the genome. sulphide:quinone reductase and denitrification may serve as detoxification systems allowing L. profundi LP1 T to thrive in a sulphide and nitrate enriched environment. The information gained from the genome gives a greater insight in the functional role of L. profundi LP1 T in the biofilm and its adaption strategy in an extreme environment. Other/Unknown Material Arctic BioMed Central Arctic |
institution |
Open Polar |
collection |
BioMed Central |
op_collection_id |
ftbiomed |
language |
English |
topic |
Lutibacter Flavobacteriaceae Loki’s castle Biofilm Deep-sea hydrothermal vent |
spellingShingle |
Lutibacter Flavobacteriaceae Loki’s castle Biofilm Deep-sea hydrothermal vent Wissuwa, Juliane Bauer, Sven Steen, Ida Stokke, Runar Complete genome sequence of Lutibacter profundi LP1 T isolated from an Arctic deep-sea hydrothermal vent system |
topic_facet |
Lutibacter Flavobacteriaceae Loki’s castle Biofilm Deep-sea hydrothermal vent |
description |
Abstract Lutibacter profundi LP1 T within the family Flavobacteriaceae was isolated from a biofilm growing on the surface of a black smoker chimney at the Loki’s Castle vent field, located on the Arctic Mid-Ocean Ridge. The complete genome of L. profundi LP1 T is the first genome to be published within the genus Lutibacter. L. profundi LP1 T consists of a single 2,966,978 bp circular chromosome with a GC content of 29.8%. The genome comprises 2,537 protein-coding genes, 40 tRNA species and 2 rRNA operons. The microaerophilic, organotrophic isolate contains genes for all central carbohydrate metabolic pathways. However, genes for the oxidative branch of the pentose-phosphate-pathway, the glyoxylate shunt of the tricarboxylic acid cycle and the ATP citrate lyase for reverse TCA are not present. L. profundi LP1 T utilizes starch, sucrose and diverse proteinous carbon sources. In accordance, the genome harbours 130 proteases and 104 carbohydrate-active enzymes, indicating a specialization in degrading organic matter. Among a small arsenal of 24 glycosyl hydrolases, which offer the possibility to hydrolyse diverse poly- and oligosaccharides, a starch utilization cluster was identified. Furthermore, a variety of enzymes may be secreted via T9SS and contribute to the hydrolytic variety of the microorganism. Genes for gliding motility are present, which may enable the bacteria to move within the biofilm. A substantial number of genes encoding for extracellular polysaccharide synthesis pathways, curli fibres and attachment to surfaces could mediate adhesion in the biofilm and may contribute to the biofilm formation. In addition to aerobic respiration, the complete denitrification pathway and genes for sulphide oxidation e.g. sulphide:quinone reductase are present in the genome. sulphide:quinone reductase and denitrification may serve as detoxification systems allowing L. profundi LP1 T to thrive in a sulphide and nitrate enriched environment. The information gained from the genome gives a greater insight in the functional role of L. profundi LP1 T in the biofilm and its adaption strategy in an extreme environment. |
format |
Other/Unknown Material |
author |
Wissuwa, Juliane Bauer, Sven Steen, Ida Stokke, Runar |
author_facet |
Wissuwa, Juliane Bauer, Sven Steen, Ida Stokke, Runar |
author_sort |
Wissuwa, Juliane |
title |
Complete genome sequence of Lutibacter profundi LP1 T isolated from an Arctic deep-sea hydrothermal vent system |
title_short |
Complete genome sequence of Lutibacter profundi LP1 T isolated from an Arctic deep-sea hydrothermal vent system |
title_full |
Complete genome sequence of Lutibacter profundi LP1 T isolated from an Arctic deep-sea hydrothermal vent system |
title_fullStr |
Complete genome sequence of Lutibacter profundi LP1 T isolated from an Arctic deep-sea hydrothermal vent system |
title_full_unstemmed |
Complete genome sequence of Lutibacter profundi LP1 T isolated from an Arctic deep-sea hydrothermal vent system |
title_sort |
complete genome sequence of lutibacter profundi lp1 t isolated from an arctic deep-sea hydrothermal vent system |
publisher |
BioMed Central Ltd. |
publishDate |
2017 |
url |
http://www.standardsingenomics.com/content/12/1/5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://www.standardsingenomics.com/content/12/1/5 |
op_rights |
Copyright 2017 The Author(s). |
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1766335692467601408 |