Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO 2 stress response
Abstract Background Human activities have led to a substantial increase in carbon dioxide (CO 2 ) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated p CO 2 . Results A total o...
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ftbiomed:oai:biomedcentral.com:s40665-016-0019-y 2023-05-15T17:51:48+02:00 Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO 2 stress response Ertl, Nicole O’Connor, Wayne Wiegand, Aaron Elizur, Abigail 2016-09-19 http://www.climatechangeresponses.com/content/3/1/6 en eng BioMed Central Ltd. http://www.climatechangeresponses.com/content/3/1/6 Copyright 2016 The Author(s). Saccostrea glomerata Sydney rock oyster Molluscs RNA-seq Stress Carbon dioxide Immunity Biomineralisation Research 2016 ftbiomed 2016-10-09T00:01:46Z Abstract Background Human activities have led to a substantial increase in carbon dioxide (CO 2 ) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated p CO 2 . Results A total of 1626 S. glomerata transcripts were found to be differentially expressed in oysters exposed to elevated p CO 2 when compared to control oysters. These transcripts cover a range of functions, from immunity (e.g. pattern recognition receptors, antimicrobial peptides), to respiration (e.g. antioxidants, mitochondrial respiratory chain proteins) and biomineralisation (e.g. carbonic anhydrase). Overall, elevated levels of CO 2 appear to have resulted in a priming of the immune system and in producing countermeasures to potential oxidative stress. CO 2 exposure also seems to have resulted in an increase in the expression of proteins involved in protein synthesis, whereas transcripts putatively coding for proteins with a role in cilia and flagella function were down-regulated in response to the stressor. In addition, while some of the transcripts related to biomineralisation were up-regulated (e.g. carbonic anhydrase 2, alkaline phosphatase), a small group was down-regulated (e.g. perlucin). Conclusions This study highlighted the complex molecular response of the bivalve S. glomerata to expected near-future ocean acidification levels. While there are indications that the oyster attempted to adapt to the stressor, gauged by immune system priming and the increase in protein synthesis, some processes such cilia function appear to have been negatively affected by the elevated levels of CO 2 . Other/Unknown Material Ocean acidification BioMed Central |
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BioMed Central |
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English |
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Saccostrea glomerata Sydney rock oyster Molluscs RNA-seq Stress Carbon dioxide Immunity Biomineralisation |
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Saccostrea glomerata Sydney rock oyster Molluscs RNA-seq Stress Carbon dioxide Immunity Biomineralisation Ertl, Nicole O’Connor, Wayne Wiegand, Aaron Elizur, Abigail Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO 2 stress response |
topic_facet |
Saccostrea glomerata Sydney rock oyster Molluscs RNA-seq Stress Carbon dioxide Immunity Biomineralisation |
description |
Abstract Background Human activities have led to a substantial increase in carbon dioxide (CO 2 ) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated p CO 2 . Results A total of 1626 S. glomerata transcripts were found to be differentially expressed in oysters exposed to elevated p CO 2 when compared to control oysters. These transcripts cover a range of functions, from immunity (e.g. pattern recognition receptors, antimicrobial peptides), to respiration (e.g. antioxidants, mitochondrial respiratory chain proteins) and biomineralisation (e.g. carbonic anhydrase). Overall, elevated levels of CO 2 appear to have resulted in a priming of the immune system and in producing countermeasures to potential oxidative stress. CO 2 exposure also seems to have resulted in an increase in the expression of proteins involved in protein synthesis, whereas transcripts putatively coding for proteins with a role in cilia and flagella function were down-regulated in response to the stressor. In addition, while some of the transcripts related to biomineralisation were up-regulated (e.g. carbonic anhydrase 2, alkaline phosphatase), a small group was down-regulated (e.g. perlucin). Conclusions This study highlighted the complex molecular response of the bivalve S. glomerata to expected near-future ocean acidification levels. While there are indications that the oyster attempted to adapt to the stressor, gauged by immune system priming and the increase in protein synthesis, some processes such cilia function appear to have been negatively affected by the elevated levels of CO 2 . |
format |
Other/Unknown Material |
author |
Ertl, Nicole O’Connor, Wayne Wiegand, Aaron Elizur, Abigail |
author_facet |
Ertl, Nicole O’Connor, Wayne Wiegand, Aaron Elizur, Abigail |
author_sort |
Ertl, Nicole |
title |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO 2 stress response |
title_short |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO 2 stress response |
title_full |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO 2 stress response |
title_fullStr |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO 2 stress response |
title_full_unstemmed |
Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO 2 stress response |
title_sort |
molecular analysis of the sydney rock oyster (saccostrea glomerata) co 2 stress response |
publisher |
BioMed Central Ltd. |
publishDate |
2016 |
url |
http://www.climatechangeresponses.com/content/3/1/6 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
http://www.climatechangeresponses.com/content/3/1/6 |
op_rights |
Copyright 2016 The Author(s). |
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1766159069821796352 |