Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt
Abstract Background Mine tailings, containing metals and production chemicals such as flotation chemicals and flocculants, may pose an environmental threat to aquatic organisms living in downstream ecosystems. The aim of this work was to study to which degree Lilaflot D817M, a flotation chemical ext...
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ftbiomed:oai:biomedcentral.com:s13104-015-1342-2 2023-05-15T15:30:50+02:00 Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt Olsvik, Pål Urke, Henning Nilsen, Tom Ulvund, John Kristensen, Torstein 2015-08-29 http://www.biomedcentral.com/1756-0500/8/389 en eng BioMed Central Ltd. http://www.biomedcentral.com/1756-0500/8/389 Copyright 2015 Olsvik et al. Mining activity Atlantic salmon Flotation chemicals Lilaflot D817M Transcriptional responses Research article 2015 ftbiomed 2015-08-30T00:05:14Z Abstract Background Mine tailings, containing metals and production chemicals such as flotation chemicals and flocculants, may pose an environmental threat to aquatic organisms living in downstream ecosystems. The aim of this work was to study to which degree Lilaflot D817M, a flotation chemical extensively used by the mining industry, represents a hazard for migrating salmon in rivers affected by mining activity. Smoltifying Atlantic salmon were exposed to four concentrations of iron-ore mine tailings containing residual Lilaflot D817M [water versus tailing volumes of 0.002 (Low), 0.004 (Medium), 0.013 (High) and 0.04 (Max)]. After 96 h of exposure, gill and liver tissues were harvested for transcriptional responses. Target genes included markers for oxidative stress, detoxification, apoptosis and DNA repair, cell signaling and growth. Results Of the 16 evaluated markers, significant transcriptional responses of exposure to tailings enriched with Lilaflot D817M were observed for CYP1A, HSP70 and HMOX1 in liver tissue and CYP1A in gill tissue. The significant induction of CYP1A in both liver and gills suggest that the flotation chemical is taken up by the fish and activates cytochrome P450 detoxification via phase I biotransformation in the cells. Conclusions The overall weak transcriptional responses to short-term exposure to Lilaflot D817M-containing iron-ore tailings suggest that the mining chemical has relatively low toxic effect on fish. The underlying mechanisms behind the observed CYP1A induction should be studied further. Article in Journal/Newspaper Atlantic salmon BioMed Central |
institution |
Open Polar |
collection |
BioMed Central |
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ftbiomed |
language |
English |
topic |
Mining activity Atlantic salmon Flotation chemicals Lilaflot D817M Transcriptional responses |
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Mining activity Atlantic salmon Flotation chemicals Lilaflot D817M Transcriptional responses Olsvik, Pål Urke, Henning Nilsen, Tom Ulvund, John Kristensen, Torstein Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt |
topic_facet |
Mining activity Atlantic salmon Flotation chemicals Lilaflot D817M Transcriptional responses |
description |
Abstract Background Mine tailings, containing metals and production chemicals such as flotation chemicals and flocculants, may pose an environmental threat to aquatic organisms living in downstream ecosystems. The aim of this work was to study to which degree Lilaflot D817M, a flotation chemical extensively used by the mining industry, represents a hazard for migrating salmon in rivers affected by mining activity. Smoltifying Atlantic salmon were exposed to four concentrations of iron-ore mine tailings containing residual Lilaflot D817M [water versus tailing volumes of 0.002 (Low), 0.004 (Medium), 0.013 (High) and 0.04 (Max)]. After 96 h of exposure, gill and liver tissues were harvested for transcriptional responses. Target genes included markers for oxidative stress, detoxification, apoptosis and DNA repair, cell signaling and growth. Results Of the 16 evaluated markers, significant transcriptional responses of exposure to tailings enriched with Lilaflot D817M were observed for CYP1A, HSP70 and HMOX1 in liver tissue and CYP1A in gill tissue. The significant induction of CYP1A in both liver and gills suggest that the flotation chemical is taken up by the fish and activates cytochrome P450 detoxification via phase I biotransformation in the cells. Conclusions The overall weak transcriptional responses to short-term exposure to Lilaflot D817M-containing iron-ore tailings suggest that the mining chemical has relatively low toxic effect on fish. The underlying mechanisms behind the observed CYP1A induction should be studied further. |
format |
Article in Journal/Newspaper |
author |
Olsvik, Pål Urke, Henning Nilsen, Tom Ulvund, John Kristensen, Torstein |
author_facet |
Olsvik, Pål Urke, Henning Nilsen, Tom Ulvund, John Kristensen, Torstein |
author_sort |
Olsvik, Pål |
title |
Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt |
title_short |
Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt |
title_full |
Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt |
title_fullStr |
Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt |
title_full_unstemmed |
Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt |
title_sort |
effects of mining chemicals on fish: exposure to tailings containing lilaflot d817m induces cyp1a transcription in atlantic salmon smolt |
publisher |
BioMed Central Ltd. |
publishDate |
2015 |
url |
http://www.biomedcentral.com/1756-0500/8/389 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.biomedcentral.com/1756-0500/8/389 |
op_rights |
Copyright 2015 Olsvik et al. |
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1766361307842347008 |