Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria
Abstract Background Both Dirofilaria repens and recently D. immitis are known to be endemic in Hungary. As one of several recent cases, the fatal case of a dog infested with D. immitis in Szeged, Southern Hungary , received attention from the media. Hence it was decided to catch mosquitoes in the ga...
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ftbiomed:oai:biomedcentral.com:s13071-015-0637-4 2023-05-15T18:40:08+02:00 Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria Zittra, Carina Kocziha, Zsanett Pinnyei, Szilárd Harl, Josef Kieser, Katrin Laciny, Alice Eigner, Barbara Silbermayr, Katja Duscher, Georg G Fok, Éva Fuehrer, Hans-Peter 2015-01-13 http://www.parasitesandvectors.com/content/8/1/16 en eng BioMed Central Ltd. http://www.parasitesandvectors.com/content/8/1/16 Copyright 2015 Zittra et al.; licensee BioMed Central. Culicidae Dirofilaria Setaria tundra Blood-fed Avian malaria Cytochrome c oxidase I Cytochrome b Hungary Research 2015 ftbiomed 2015-01-25T00:50:04Z Abstract Background Both Dirofilaria repens and recently D. immitis are known to be endemic in Hungary. As one of several recent cases, the fatal case of a dog infested with D. immitis in Szeged, Southern Hungary , received attention from the media. Hence it was decided to catch mosquitoes in the garden where the dog lived to screen for filarioid helminths and Plasmodium spp. using molecular tools. Methods Mosquitoes were caught in Szeged, in the garden where the infected dog was kept, in July 2013 with M-360 electric mosquito traps and were stored in ethanol until further procedure. Female mosquitoes were classified to genus level by morphology. Each mosquito was homogenized and analyzed for filarioid helminths and avian malaria using standardized PCR techniques. Positive mosquito samples were further identified to species level by comparing a section of the mitochondrial COI gene to GenBank ® entries. Results In this study, 267 blood-fed mosquitoes were caught in July 2013 in Szeged. Subsequent molecular screening revealed that not only D. immitis was present in the analyzed specimens but also DNA of D. repens , Setaria tundra and Plasmodium spp. was confirmed. Conclusions The analysis of blood-fed mosquitoes for the diagnosis of Dirofilaria spp. and other mosquito-borne pathogens seems to be an adequate technique to evaluate if filarioid helminths are present in a certain area. Usually only unfed female mosquitoes are analyzed for epidemiological studies. However, blood-fed mosquitoes can only be used for screening if a pathogen is present because the role of the mosquito as vector cannot be classified (blood of bitten host). Furthermore, Setaria tundra was confirmed for the first time in Hungary. Other/Unknown Material Tundra BioMed Central |
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BioMed Central |
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English |
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Culicidae Dirofilaria Setaria tundra Blood-fed Avian malaria Cytochrome c oxidase I Cytochrome b Hungary |
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Culicidae Dirofilaria Setaria tundra Blood-fed Avian malaria Cytochrome c oxidase I Cytochrome b Hungary Zittra, Carina Kocziha, Zsanett Pinnyei, Szilárd Harl, Josef Kieser, Katrin Laciny, Alice Eigner, Barbara Silbermayr, Katja Duscher, Georg G Fok, Éva Fuehrer, Hans-Peter Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
topic_facet |
Culicidae Dirofilaria Setaria tundra Blood-fed Avian malaria Cytochrome c oxidase I Cytochrome b Hungary |
description |
Abstract Background Both Dirofilaria repens and recently D. immitis are known to be endemic in Hungary. As one of several recent cases, the fatal case of a dog infested with D. immitis in Szeged, Southern Hungary , received attention from the media. Hence it was decided to catch mosquitoes in the garden where the dog lived to screen for filarioid helminths and Plasmodium spp. using molecular tools. Methods Mosquitoes were caught in Szeged, in the garden where the infected dog was kept, in July 2013 with M-360 electric mosquito traps and were stored in ethanol until further procedure. Female mosquitoes were classified to genus level by morphology. Each mosquito was homogenized and analyzed for filarioid helminths and avian malaria using standardized PCR techniques. Positive mosquito samples were further identified to species level by comparing a section of the mitochondrial COI gene to GenBank ® entries. Results In this study, 267 blood-fed mosquitoes were caught in July 2013 in Szeged. Subsequent molecular screening revealed that not only D. immitis was present in the analyzed specimens but also DNA of D. repens , Setaria tundra and Plasmodium spp. was confirmed. Conclusions The analysis of blood-fed mosquitoes for the diagnosis of Dirofilaria spp. and other mosquito-borne pathogens seems to be an adequate technique to evaluate if filarioid helminths are present in a certain area. Usually only unfed female mosquitoes are analyzed for epidemiological studies. However, blood-fed mosquitoes can only be used for screening if a pathogen is present because the role of the mosquito as vector cannot be classified (blood of bitten host). Furthermore, Setaria tundra was confirmed for the first time in Hungary. |
format |
Other/Unknown Material |
author |
Zittra, Carina Kocziha, Zsanett Pinnyei, Szilárd Harl, Josef Kieser, Katrin Laciny, Alice Eigner, Barbara Silbermayr, Katja Duscher, Georg G Fok, Éva Fuehrer, Hans-Peter |
author_facet |
Zittra, Carina Kocziha, Zsanett Pinnyei, Szilárd Harl, Josef Kieser, Katrin Laciny, Alice Eigner, Barbara Silbermayr, Katja Duscher, Georg G Fok, Éva Fuehrer, Hans-Peter |
author_sort |
Zittra, Carina |
title |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_short |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_full |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_fullStr |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_full_unstemmed |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_sort |
screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
publisher |
BioMed Central Ltd. |
publishDate |
2015 |
url |
http://www.parasitesandvectors.com/content/8/1/16 |
genre |
Tundra |
genre_facet |
Tundra |
op_relation |
http://www.parasitesandvectors.com/content/8/1/16 |
op_rights |
Copyright 2015 Zittra et al.; licensee BioMed Central. |
_version_ |
1766229332113489920 |