Characterization of a new genotype of avian bornavirus from wild ducks

Abstract Background Avian bornaviruses (ABV) are a recently described group of intranuclear negative-stranded RNA viruses (Order Mononegavirales , Family Bornaviridae ). At least 13 different ABV genotypes have been described. One genotype, the Canada goose genotype (ABV-CG), has been isolated from...

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Main Authors: Guo, Jianhua, Shivaprasad, H L, Rech, Raquel R, Heatley, Jill J, Tizard, Ian, Payne, Susan
Format: Other/Unknown Material
Language:English
Published: BioMed Central Ltd. 2014
Subjects:
Online Access:http://www.virologyj.com/content/11/1/197
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spelling ftbiomed:oai:biomedcentral.com:s12985-014-0197-9 2023-05-15T15:48:57+02:00 Characterization of a new genotype of avian bornavirus from wild ducks Guo, Jianhua Shivaprasad, H L Rech, Raquel R Heatley, Jill J Tizard, Ian Payne, Susan 2014-11-19 http://www.virologyj.com/content/11/1/197 en eng BioMed Central Ltd. http://www.virologyj.com/content/11/1/197 Copyright 2014 Guo et al.; licensee BioMed Central Ltd. Avian bornavirus Genotype Mallard Research 2014 ftbiomed 2014-11-23T00:44:44Z Abstract Background Avian bornaviruses (ABV) are a recently described group of intranuclear negative-stranded RNA viruses (Order Mononegavirales , Family Bornaviridae ). At least 13 different ABV genotypes have been described. One genotype, the Canada goose genotype (ABV-CG), has been isolated from geese and swans and is widely distributed across North America. Results We have isolated and characterized a previously undescribed genotype of avian bornavirus from the brains of wild ducks. This new genotype, provisionally designated ABV genotype MALL, was detected in 12 of 83 mallards, and 1 of 8 wood ducks collected at a single location in central Oklahoma. The virus was cultured on primary duck embryo fibroblasts, fragments were cloned, and its genome sequence of 8904 nucleotides determined. This new genotype has 72% nucleotide identity and 83% amino acid identity with the ABV-CG genotype previously shown to be present in geese and swans. Histologic and immunohistochemical examination of the brains and eyes of four positive ducks indicated the presence of virus-infected neurons and glia in their cerebrums and retinas in the absence of inflammation. Conclusions More than one genotype of ABV is circulating in North American waterfowl. While the infected ducks were not observed to be suffering from overt disease, based on the immunohistochemistry, we speculate that they may have suffered some visual impairment. Other/Unknown Material Canada Goose BioMed Central Canada
institution Open Polar
collection BioMed Central
op_collection_id ftbiomed
language English
topic Avian bornavirus
Genotype
Mallard
spellingShingle Avian bornavirus
Genotype
Mallard
Guo, Jianhua
Shivaprasad, H L
Rech, Raquel R
Heatley, Jill J
Tizard, Ian
Payne, Susan
Characterization of a new genotype of avian bornavirus from wild ducks
topic_facet Avian bornavirus
Genotype
Mallard
description Abstract Background Avian bornaviruses (ABV) are a recently described group of intranuclear negative-stranded RNA viruses (Order Mononegavirales , Family Bornaviridae ). At least 13 different ABV genotypes have been described. One genotype, the Canada goose genotype (ABV-CG), has been isolated from geese and swans and is widely distributed across North America. Results We have isolated and characterized a previously undescribed genotype of avian bornavirus from the brains of wild ducks. This new genotype, provisionally designated ABV genotype MALL, was detected in 12 of 83 mallards, and 1 of 8 wood ducks collected at a single location in central Oklahoma. The virus was cultured on primary duck embryo fibroblasts, fragments were cloned, and its genome sequence of 8904 nucleotides determined. This new genotype has 72% nucleotide identity and 83% amino acid identity with the ABV-CG genotype previously shown to be present in geese and swans. Histologic and immunohistochemical examination of the brains and eyes of four positive ducks indicated the presence of virus-infected neurons and glia in their cerebrums and retinas in the absence of inflammation. Conclusions More than one genotype of ABV is circulating in North American waterfowl. While the infected ducks were not observed to be suffering from overt disease, based on the immunohistochemistry, we speculate that they may have suffered some visual impairment.
format Other/Unknown Material
author Guo, Jianhua
Shivaprasad, H L
Rech, Raquel R
Heatley, Jill J
Tizard, Ian
Payne, Susan
author_facet Guo, Jianhua
Shivaprasad, H L
Rech, Raquel R
Heatley, Jill J
Tizard, Ian
Payne, Susan
author_sort Guo, Jianhua
title Characterization of a new genotype of avian bornavirus from wild ducks
title_short Characterization of a new genotype of avian bornavirus from wild ducks
title_full Characterization of a new genotype of avian bornavirus from wild ducks
title_fullStr Characterization of a new genotype of avian bornavirus from wild ducks
title_full_unstemmed Characterization of a new genotype of avian bornavirus from wild ducks
title_sort characterization of a new genotype of avian bornavirus from wild ducks
publisher BioMed Central Ltd.
publishDate 2014
url http://www.virologyj.com/content/11/1/197
geographic Canada
geographic_facet Canada
genre Canada Goose
genre_facet Canada Goose
op_relation http://www.virologyj.com/content/11/1/197
op_rights Copyright 2014 Guo et al.; licensee BioMed Central Ltd.
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