Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories
Abstract Background Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocomp...
Main Authors: | , , , , |
---|---|
Format: | Other/Unknown Material |
Language: | English |
Published: |
BioMed Central Ltd.
2016
|
Subjects: | |
Online Access: | http://www.microbialcellfactories.com/content/15/1/76 |
id |
ftbiomed:oai:biomedcentral.com:s12934-016-0477-8 |
---|---|
record_format |
openpolar |
spelling |
ftbiomed:oai:biomedcentral.com:s12934-016-0477-8 2023-05-15T13:39:16+02:00 Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories Plaza, D. Gallardo, C. Straub, Y. Bravo, D. Pérez-Donoso, J. 2016-05-06 http://www.microbialcellfactories.com/content/15/1/76 en eng BioMed Central Ltd. http://www.microbialcellfactories.com/content/15/1/76 Copyright 2016 Plaza et al. Fluorescent nanoparticles Quantum dots Green synthesis Antarctica Bacteria Heavy metals Research 2016 ftbiomed 2016-05-07T23:59:55Z Abstract Background Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocompatibility and avoid the generation of toxic byproducts. The use of biological systems, particularly prokaryotes, has emerged as a promising alternative. Recent studies indicate that QDs biosynthesis is related to factors such as cellular redox status and antioxidant defenses. Based on this, the mixture of extreme conditions of Antarctica would allow the development of natural QDs producing bacteria. Results In this study we isolated and characterized cadmium and tellurite resistant Antarctic bacteria capable of synthesizing CdS and CdTe QDs when exposed to these oxidizing heavy metals. A time dependent change in fluorescence emission color, moving from green to red, was determined on bacterial cells exposed to metals. Biosynthesis was observed in cells grown at different temperatures and high metal concentrations. Electron microscopy analysis of treated cells revealed nanometric electron-dense elements and structures resembling membrane vesicles mostly associated to periplasmic space. Purified biosynthesized QDs displayed broad absorption and emission spectra characteristic of biogenic Cd nanoparticles. Conclusions Our work presents a novel and simple biological approach to produce QDs at room temperature by using heavy metal resistant Antarctic bacteria, highlighting the unique properties of these microorganisms as potent natural producers of nano-scale materials and promising candidates for bioremediation purposes. Other/Unknown Material Antarc* Antarctic Antarctica BioMed Central Antarctic |
institution |
Open Polar |
collection |
BioMed Central |
op_collection_id |
ftbiomed |
language |
English |
topic |
Fluorescent nanoparticles Quantum dots Green synthesis Antarctica Bacteria Heavy metals |
spellingShingle |
Fluorescent nanoparticles Quantum dots Green synthesis Antarctica Bacteria Heavy metals Plaza, D. Gallardo, C. Straub, Y. Bravo, D. Pérez-Donoso, J. Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
topic_facet |
Fluorescent nanoparticles Quantum dots Green synthesis Antarctica Bacteria Heavy metals |
description |
Abstract Background Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocompatibility and avoid the generation of toxic byproducts. The use of biological systems, particularly prokaryotes, has emerged as a promising alternative. Recent studies indicate that QDs biosynthesis is related to factors such as cellular redox status and antioxidant defenses. Based on this, the mixture of extreme conditions of Antarctica would allow the development of natural QDs producing bacteria. Results In this study we isolated and characterized cadmium and tellurite resistant Antarctic bacteria capable of synthesizing CdS and CdTe QDs when exposed to these oxidizing heavy metals. A time dependent change in fluorescence emission color, moving from green to red, was determined on bacterial cells exposed to metals. Biosynthesis was observed in cells grown at different temperatures and high metal concentrations. Electron microscopy analysis of treated cells revealed nanometric electron-dense elements and structures resembling membrane vesicles mostly associated to periplasmic space. Purified biosynthesized QDs displayed broad absorption and emission spectra characteristic of biogenic Cd nanoparticles. Conclusions Our work presents a novel and simple biological approach to produce QDs at room temperature by using heavy metal resistant Antarctic bacteria, highlighting the unique properties of these microorganisms as potent natural producers of nano-scale materials and promising candidates for bioremediation purposes. |
format |
Other/Unknown Material |
author |
Plaza, D. Gallardo, C. Straub, Y. Bravo, D. Pérez-Donoso, J. |
author_facet |
Plaza, D. Gallardo, C. Straub, Y. Bravo, D. Pérez-Donoso, J. |
author_sort |
Plaza, D. |
title |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_short |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_full |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_fullStr |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_full_unstemmed |
Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories |
title_sort |
biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant antarctic bacteria: exploring novel natural nanofactories |
publisher |
BioMed Central Ltd. |
publishDate |
2016 |
url |
http://www.microbialcellfactories.com/content/15/1/76 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://www.microbialcellfactories.com/content/15/1/76 |
op_rights |
Copyright 2016 Plaza et al. |
_version_ |
1766116621870432256 |