Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA

• Main Authors: Henningsson, Anna, Hvidsten, Dag, Kristiansen, Bjørn-Erik, Matussek, Andreas, Stuen, Snorre, Jenkins, Andrew
• Format: Article in Journal/Newspaper
• Language: English
• Published: BioMed Central Ltd. 2015
• Subjects: Anaplasma phagocytophilum; Ixodes ricinus; TaqMan realtime PCR; Norway; gltA; Prevalence; Arctic; Northern Norway
• Online Access: http://www.biomedcentral.com/1471-2180/15/153

Abstract Background A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA , was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway ( n = 669) and Telemark county in southern Norway ( n = 96). Results Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % ( p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. Conclusion This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.