Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA
Abstract Background A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA , was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway ( n = 669) and Telemark county in southern Norway ( n =...
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ftbiomed:oai:biomedcentral.com:s12866-015-0486-5 2023-05-15T15:03:02+02:00 Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA Henningsson, Anna Hvidsten, Dag Kristiansen, Bjørn-Erik Matussek, Andreas Stuen, Snorre Jenkins, Andrew 2015-08-01 http://www.biomedcentral.com/1471-2180/15/153 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-2180/15/153 Copyright 2015 Henningsson et al. Anaplasma phagocytophilum Ixodes ricinus TaqMan realtime PCR Norway gltA Prevalence Research article 2015 ftbiomed 2015-08-02T00:01:31Z Abstract Background A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA , was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway ( n = 669) and Telemark county in southern Norway ( n = 96). Results Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % ( p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. Conclusion This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway. Article in Journal/Newspaper Arctic Northern Norway BioMed Central Arctic Norway |
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language |
English |
topic |
Anaplasma phagocytophilum Ixodes ricinus TaqMan realtime PCR Norway gltA Prevalence |
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Anaplasma phagocytophilum Ixodes ricinus TaqMan realtime PCR Norway gltA Prevalence Henningsson, Anna Hvidsten, Dag Kristiansen, Bjørn-Erik Matussek, Andreas Stuen, Snorre Jenkins, Andrew Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
topic_facet |
Anaplasma phagocytophilum Ixodes ricinus TaqMan realtime PCR Norway gltA Prevalence |
description |
Abstract Background A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA , was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway ( n = 669) and Telemark county in southern Norway ( n = 96). Results Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % ( p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. Conclusion This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway. |
format |
Article in Journal/Newspaper |
author |
Henningsson, Anna Hvidsten, Dag Kristiansen, Bjørn-Erik Matussek, Andreas Stuen, Snorre Jenkins, Andrew |
author_facet |
Henningsson, Anna Hvidsten, Dag Kristiansen, Bjørn-Erik Matussek, Andreas Stuen, Snorre Jenkins, Andrew |
author_sort |
Henningsson, Anna |
title |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_short |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_full |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_fullStr |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_full_unstemmed |
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA |
title_sort |
detection of anaplasma phagocytophilum in ixodes ricinus ticks from norway using a realtime pcr assay targeting the anaplasma citrate synthase gene glta |
publisher |
BioMed Central Ltd. |
publishDate |
2015 |
url |
http://www.biomedcentral.com/1471-2180/15/153 |
geographic |
Arctic Norway |
geographic_facet |
Arctic Norway |
genre |
Arctic Northern Norway |
genre_facet |
Arctic Northern Norway |
op_relation |
http://www.biomedcentral.com/1471-2180/15/153 |
op_rights |
Copyright 2015 Henningsson et al. |
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1766334933977006080 |