A transcriptional timetable of autumn senescence
Abstract Background We have developed genomic tools to allow the genus Populus (aspens and cottonwoods) to be exploited as a full-featured model for investigating fundamental aspects of tree biology. We have undertaken large-scale expressed sequence tag (EST) sequencing programs and created Populus...
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ftbiomed:oai:biomedcentral.com:gb-2004-5-4-r24 2023-05-15T17:44:50+02:00 A transcriptional timetable of autumn senescence Andersson, Anders Keskitalo, Johanna Sjödin, Andreas Bhalerao, Rupali Sterky, Fredrik Wissel, Kirsten Tandre, Karolina Aspeborg, Henrik Moyle, Richard Ohmiya, Yasunori Bhalerao, Rishikesh Brunner, Amy Gustafsson, Petter Karlsson, Jan Lundeberg, Joakim Nilsson, Ove Sandberg, Göran Strauss, Steven Sundberg, Björn Uhlen, Mathias Jansson, Stefan Nilsson, Peter 2004-03-10 http://genomebiology.com/2004/5/4/R24 en eng BioMed Central Ltd. http://genomebiology.com/2004/5/4/R24 Copyright 2004 Andersson et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Research 2004 ftbiomed 2007-11-11T15:39:09Z Abstract Background We have developed genomic tools to allow the genus Populus (aspens and cottonwoods) to be exploited as a full-featured model for investigating fundamental aspects of tree biology. We have undertaken large-scale expressed sequence tag (EST) sequencing programs and created Populus microarrays with significant gene coverage. One of the important aspects of plant biology that cannot be studied in annual plants is the gene activity involved in the induction of autumn leaf senescence. Results On the basis of 36,354 Populus ESTs, obtained from seven cDNA libraries, we have created a DNA microarray consisting of 13,490 clones, spotted in duplicate. Of these clones, 12,376 (92%) were confirmed by resequencing and all sequences were annotated and functionally classified. Here we have used the microarray to study transcript abundance in leaves of a free-growing aspen tree ( Populus tremula ) in northern Sweden during natural autumn senescence. Of the 13,490 spotted clones, 3,792 represented genes with significant expression in all leaf samples from the seven studied dates. Conclusions We observed a major shift in gene expression, coinciding with massive chlorophyll degradation, that reflected a shift from photosynthetic competence to energy generation by mitochondrial respiration, oxidation of fatty acids and nutrient mobilization. Autumn senescence had much in common with senescence in annual plants; for example many proteases were induced. We also found evidence for increased transcriptional activity before the appearance of visible signs of senescence, presumably preparing the leaf for degradation of its components. Other/Unknown Material Northern Sweden BioMed Central |
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Open Polar |
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BioMed Central |
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ftbiomed |
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English |
description |
Abstract Background We have developed genomic tools to allow the genus Populus (aspens and cottonwoods) to be exploited as a full-featured model for investigating fundamental aspects of tree biology. We have undertaken large-scale expressed sequence tag (EST) sequencing programs and created Populus microarrays with significant gene coverage. One of the important aspects of plant biology that cannot be studied in annual plants is the gene activity involved in the induction of autumn leaf senescence. Results On the basis of 36,354 Populus ESTs, obtained from seven cDNA libraries, we have created a DNA microarray consisting of 13,490 clones, spotted in duplicate. Of these clones, 12,376 (92%) were confirmed by resequencing and all sequences were annotated and functionally classified. Here we have used the microarray to study transcript abundance in leaves of a free-growing aspen tree ( Populus tremula ) in northern Sweden during natural autumn senescence. Of the 13,490 spotted clones, 3,792 represented genes with significant expression in all leaf samples from the seven studied dates. Conclusions We observed a major shift in gene expression, coinciding with massive chlorophyll degradation, that reflected a shift from photosynthetic competence to energy generation by mitochondrial respiration, oxidation of fatty acids and nutrient mobilization. Autumn senescence had much in common with senescence in annual plants; for example many proteases were induced. We also found evidence for increased transcriptional activity before the appearance of visible signs of senescence, presumably preparing the leaf for degradation of its components. |
format |
Other/Unknown Material |
author |
Andersson, Anders Keskitalo, Johanna Sjödin, Andreas Bhalerao, Rupali Sterky, Fredrik Wissel, Kirsten Tandre, Karolina Aspeborg, Henrik Moyle, Richard Ohmiya, Yasunori Bhalerao, Rishikesh Brunner, Amy Gustafsson, Petter Karlsson, Jan Lundeberg, Joakim Nilsson, Ove Sandberg, Göran Strauss, Steven Sundberg, Björn Uhlen, Mathias Jansson, Stefan Nilsson, Peter |
spellingShingle |
Andersson, Anders Keskitalo, Johanna Sjödin, Andreas Bhalerao, Rupali Sterky, Fredrik Wissel, Kirsten Tandre, Karolina Aspeborg, Henrik Moyle, Richard Ohmiya, Yasunori Bhalerao, Rishikesh Brunner, Amy Gustafsson, Petter Karlsson, Jan Lundeberg, Joakim Nilsson, Ove Sandberg, Göran Strauss, Steven Sundberg, Björn Uhlen, Mathias Jansson, Stefan Nilsson, Peter A transcriptional timetable of autumn senescence |
author_facet |
Andersson, Anders Keskitalo, Johanna Sjödin, Andreas Bhalerao, Rupali Sterky, Fredrik Wissel, Kirsten Tandre, Karolina Aspeborg, Henrik Moyle, Richard Ohmiya, Yasunori Bhalerao, Rishikesh Brunner, Amy Gustafsson, Petter Karlsson, Jan Lundeberg, Joakim Nilsson, Ove Sandberg, Göran Strauss, Steven Sundberg, Björn Uhlen, Mathias Jansson, Stefan Nilsson, Peter |
author_sort |
Andersson, Anders |
title |
A transcriptional timetable of autumn senescence |
title_short |
A transcriptional timetable of autumn senescence |
title_full |
A transcriptional timetable of autumn senescence |
title_fullStr |
A transcriptional timetable of autumn senescence |
title_full_unstemmed |
A transcriptional timetable of autumn senescence |
title_sort |
transcriptional timetable of autumn senescence |
publisher |
BioMed Central Ltd. |
publishDate |
2004 |
url |
http://genomebiology.com/2004/5/4/R24 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
http://genomebiology.com/2004/5/4/R24 |
op_rights |
Copyright 2004 Andersson et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
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1766147124871823360 |