An Indirect ELISA for Detection of Encephalitozoon cuniculiInfection in Farmed Blue Foxes (Alopex lagopus)
Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus) . The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed agai...
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ftbiomed:oai:biomedcentral.com:1751-0147-43-211 2023-05-15T13:19:45+02:00 An Indirect ELISA for Detection of Encephalitozoon cuniculiInfection in Farmed Blue Foxes (Alopex lagopus) Åkerstedt, J 2002-12-31 http://www.actavetscand.com/content/43/4/211 en eng BioMed Central Ltd. http://www.actavetscand.com/content/43/4/211 microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence Original article 2002 ftbiomed 2007-11-11T15:27:59Z Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus) . The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT) and carbon immunoassay (CIA) are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay) was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ = 0.99), and the ELISA and IFAT (κ = 0.958). There was no significant statistical difference between the tests (p > 0.05). It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. Article in Journal/Newspaper Alopex lagopus BioMed Central |
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BioMed Central |
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ftbiomed |
language |
English |
topic |
microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence |
spellingShingle |
microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence Åkerstedt, J An Indirect ELISA for Detection of Encephalitozoon cuniculiInfection in Farmed Blue Foxes (Alopex lagopus) |
topic_facet |
microsporidia encephalitozoonosis ELISA carbon immunoassay indirect immunofluorescence |
description |
Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause serious disease, encephalitozoonosis, in the blue fox (Alopex lagopus) . The disease diagnosis is based on clinical signs and pathological findings, and detection of E. cuniculi or circulating antibodies directed against the parasite. Indirect immunofluorescence (IFAT) and carbon immunoassay (CIA) are the most commonly used serological methods for diagnosis in this species. In the present study, an indirect ELISA (enzyme linked immunosorbent assay) was established and evaluated against IFAT by testing of 205 field samples from blue foxes. There was high agreement between the results of the ELISA and CIA (κ = 0.99), and the ELISA and IFAT (κ = 0.958). There was no significant statistical difference between the tests (p > 0.05). It was concluded that the ELISA could be used to identify seropositive farmed blue foxes. The advantage of the ELISA lies in the potential of screening large numbers of animals with the goal of eradicating E. cuniculi infection in the farms. |
format |
Article in Journal/Newspaper |
author |
Åkerstedt, J |
author_facet |
Åkerstedt, J |
author_sort |
Åkerstedt, J |
title |
An Indirect ELISA for Detection of Encephalitozoon cuniculiInfection in Farmed Blue Foxes (Alopex lagopus) |
title_short |
An Indirect ELISA for Detection of Encephalitozoon cuniculiInfection in Farmed Blue Foxes (Alopex lagopus) |
title_full |
An Indirect ELISA for Detection of Encephalitozoon cuniculiInfection in Farmed Blue Foxes (Alopex lagopus) |
title_fullStr |
An Indirect ELISA for Detection of Encephalitozoon cuniculiInfection in Farmed Blue Foxes (Alopex lagopus) |
title_full_unstemmed |
An Indirect ELISA for Detection of Encephalitozoon cuniculiInfection in Farmed Blue Foxes (Alopex lagopus) |
title_sort |
indirect elisa for detection of encephalitozoon cuniculiinfection in farmed blue foxes (alopex lagopus) |
publisher |
BioMed Central Ltd. |
publishDate |
2002 |
url |
http://www.actavetscand.com/content/43/4/211 |
genre |
Alopex lagopus |
genre_facet |
Alopex lagopus |
op_relation |
http://www.actavetscand.com/content/43/4/211 |
_version_ |
1766349385149448192 |