An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis
Abstract Background Francisellosis, caused by the bacterium Francisella noatunensis subsp. noatunensis , remains a serious threat to Atlantic cod ( Gadhus morhua ) farming in Norway and potentially in other countries. As outbreak strains appear clonal in population structure, access to highly discri...
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ftbiomed:oai:biomedcentral.com:1746-6148-9-252 2023-05-15T15:27:36+02:00 An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis Duodu, Samuel Wan, Xihe Tandstad, Nora Larsson, Pär Myrtennäs, Kerstin Sjödin, Andreas Forsman, Mats Colquhoun, Duncan J 2013-12-13 http://www.biomedcentral.com/1746-6148/9/252 en eng BioMed Central Ltd. http://www.biomedcentral.com/1746-6148/9/252 Copyright 2013 Duodu et al.; licensee BioMed Central Ltd. VNTR Capillary electrophoresis Francisella noatunensis Fish Methodology article 2013 ftbiomed 2013-12-22T01:24:31Z Abstract Background Francisellosis, caused by the bacterium Francisella noatunensis subsp. noatunensis , remains a serious threat to Atlantic cod ( Gadhus morhua ) farming in Norway and potentially in other countries. As outbreak strains appear clonal in population structure, access to highly discriminatory typing tools is critical for understanding the epidemiology of francisellosis infections in aquaculture. In this study, a simplified multiple-locus variable-number of tandem repeat analysis (MLVA) targeting five highly polymorphic variable number of tandem repeat (VNTR) loci in a single multiplex PCR was developed to rapidly discriminate between outbreak strains. Results The assay resulted in identification of at least 13 different allelic profiles or subpopulations among 91 F. noatunensis isolates from farmed cod in Norway. The VNTR loci appear relatively stable, with isolates originating from individual outbreaks showing identical MLVA profiles following repeated passage. MLVA displayed greater discriminatory power than pulse-field gel electrophoresis (PFGE). Both MLVA and PFGE show good epidemiological concordance by their abilities to separate outbreak strains from epidemiologically unrelated isolates. Conclusions The MLVA method presented here is robust, easy to perform and provides a good alternative to other typing systems for F. noatunensis subsp. noatunensis and epidemiological study of francisellosis in cod. Other/Unknown Material atlantic cod BioMed Central Norway |
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BioMed Central |
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language |
English |
topic |
VNTR Capillary electrophoresis Francisella noatunensis Fish |
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VNTR Capillary electrophoresis Francisella noatunensis Fish Duodu, Samuel Wan, Xihe Tandstad, Nora Larsson, Pär Myrtennäs, Kerstin Sjödin, Andreas Forsman, Mats Colquhoun, Duncan J An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis |
topic_facet |
VNTR Capillary electrophoresis Francisella noatunensis Fish |
description |
Abstract Background Francisellosis, caused by the bacterium Francisella noatunensis subsp. noatunensis , remains a serious threat to Atlantic cod ( Gadhus morhua ) farming in Norway and potentially in other countries. As outbreak strains appear clonal in population structure, access to highly discriminatory typing tools is critical for understanding the epidemiology of francisellosis infections in aquaculture. In this study, a simplified multiple-locus variable-number of tandem repeat analysis (MLVA) targeting five highly polymorphic variable number of tandem repeat (VNTR) loci in a single multiplex PCR was developed to rapidly discriminate between outbreak strains. Results The assay resulted in identification of at least 13 different allelic profiles or subpopulations among 91 F. noatunensis isolates from farmed cod in Norway. The VNTR loci appear relatively stable, with isolates originating from individual outbreaks showing identical MLVA profiles following repeated passage. MLVA displayed greater discriminatory power than pulse-field gel electrophoresis (PFGE). Both MLVA and PFGE show good epidemiological concordance by their abilities to separate outbreak strains from epidemiologically unrelated isolates. Conclusions The MLVA method presented here is robust, easy to perform and provides a good alternative to other typing systems for F. noatunensis subsp. noatunensis and epidemiological study of francisellosis in cod. |
format |
Other/Unknown Material |
author |
Duodu, Samuel Wan, Xihe Tandstad, Nora Larsson, Pär Myrtennäs, Kerstin Sjödin, Andreas Forsman, Mats Colquhoun, Duncan J |
author_facet |
Duodu, Samuel Wan, Xihe Tandstad, Nora Larsson, Pär Myrtennäs, Kerstin Sjödin, Andreas Forsman, Mats Colquhoun, Duncan J |
author_sort |
Duodu, Samuel |
title |
An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis |
title_short |
An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis |
title_full |
An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis |
title_fullStr |
An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis |
title_full_unstemmed |
An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis |
title_sort |
improved multiple-locus variable-number of tandem repeat analysis (mlva) for the fish pathogen francisella noatunensis using capillary electrophoresis |
publisher |
BioMed Central Ltd. |
publishDate |
2013 |
url |
http://www.biomedcentral.com/1746-6148/9/252 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_relation |
http://www.biomedcentral.com/1746-6148/9/252 |
op_rights |
Copyright 2013 Duodu et al.; licensee BioMed Central Ltd. |
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1766358017479016448 |