Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility
Abstract Background Enzymatic activity of Te lomerase R everse T ranscriptase (TERT) is important in maintaining the telomere length and has been implicated in cancer and aging related pathology. Since cancer susceptibility as well as longevity of dogs vary between breeds, this study involved sequen...
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ftbiomed:oai:biomedcentral.com:1746-6148-10-20 2023-05-15T17:22:30+02:00 Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility McAloney, Camille A Silverstein, Kevin A T Modiano, Jaime F Bagchi, Anindya 2014-01-14 http://www.biomedcentral.com/1746-6148/10/20 en eng BioMed Central Ltd. http://www.biomedcentral.com/1746-6148/10/20 Copyright 2014 McAloney et al.; licensee BioMed Central Ltd. Research article 2014 ftbiomed 2014-02-02T01:26:56Z Abstract Background Enzymatic activity of Te lomerase R everse T ranscriptase (TERT) is important in maintaining the telomere length and has been implicated in cancer and aging related pathology. Since cancer susceptibility as well as longevity of dogs vary between breeds, this study involved sequencing the entire TERT gene of Canis familiaris from DNA samples obtained from forty dogs, with ten dogs each of four breeds: Shih Tzu, Dachshund, Irish Wolfhound, and Newfoundland, each with different life expectancies and susceptibility to cancer. Results We compared the sequences of all forty individuals amongst one another and with the published sequence of canine TERT , and analyzed relationships between members of the same or different breeds. Two separate phylogenetic trees were generated and analyzed from these individuals. Polymorphisms were found most frequently in intronic regions of the gene, although exonic polymorphisms also were observed. In many locations genotypes were observed that were either homozygous for the reference sequence or heterozygous, but the variant homozygous genotype was not observed. Conclusions We propose that these homozygous variants are likely to have adverse effects in dogs. It was also found that the polymorphisms did not segregate by breed. Because the four breeds chosen come from geographically and physiologically distinct backgrounds, it can be inferred that the polymorphic diversification of TERT preceded breed derivation. Article in Journal/Newspaper Newfoundland BioMed Central |
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BioMed Central |
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ftbiomed |
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English |
description |
Abstract Background Enzymatic activity of Te lomerase R everse T ranscriptase (TERT) is important in maintaining the telomere length and has been implicated in cancer and aging related pathology. Since cancer susceptibility as well as longevity of dogs vary between breeds, this study involved sequencing the entire TERT gene of Canis familiaris from DNA samples obtained from forty dogs, with ten dogs each of four breeds: Shih Tzu, Dachshund, Irish Wolfhound, and Newfoundland, each with different life expectancies and susceptibility to cancer. Results We compared the sequences of all forty individuals amongst one another and with the published sequence of canine TERT , and analyzed relationships between members of the same or different breeds. Two separate phylogenetic trees were generated and analyzed from these individuals. Polymorphisms were found most frequently in intronic regions of the gene, although exonic polymorphisms also were observed. In many locations genotypes were observed that were either homozygous for the reference sequence or heterozygous, but the variant homozygous genotype was not observed. Conclusions We propose that these homozygous variants are likely to have adverse effects in dogs. It was also found that the polymorphisms did not segregate by breed. Because the four breeds chosen come from geographically and physiologically distinct backgrounds, it can be inferred that the polymorphic diversification of TERT preceded breed derivation. |
format |
Article in Journal/Newspaper |
author |
McAloney, Camille A Silverstein, Kevin A T Modiano, Jaime F Bagchi, Anindya |
spellingShingle |
McAloney, Camille A Silverstein, Kevin A T Modiano, Jaime F Bagchi, Anindya Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility |
author_facet |
McAloney, Camille A Silverstein, Kevin A T Modiano, Jaime F Bagchi, Anindya |
author_sort |
McAloney, Camille A |
title |
Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility |
title_short |
Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility |
title_full |
Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility |
title_fullStr |
Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility |
title_full_unstemmed |
Polymorphisms within the Telomerase Reverse Transcriptase gene (TERT) in four breeds of dogs selected for difference in lifespan and cancer susceptibility |
title_sort |
polymorphisms within the telomerase reverse transcriptase gene (tert) in four breeds of dogs selected for difference in lifespan and cancer susceptibility |
publisher |
BioMed Central Ltd. |
publishDate |
2014 |
url |
http://www.biomedcentral.com/1746-6148/10/20 |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_relation |
http://www.biomedcentral.com/1746-6148/10/20 |
op_rights |
Copyright 2014 McAloney et al.; licensee BioMed Central Ltd. |
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1766109210017267712 |