Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada

Abstract Background This study reports progress in assembling a DNA barcode reference library for Ephemeroptera, Plecoptera, and Trichoptera ("EPTs") from a Canadian subarctic site, which is the focus of a comprehensive biodiversity inventory using DNA barcoding. These three groups of aqua...

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Main Authors: Zhou, Xin, Adamowicz, Sarah J, Jacobus, Luke M, DeWalt, R Edward, Hebert, Paul DN
Format: Other/Unknown Material
Language:English
Published: BioMed Central Ltd. 2009
Subjects:
Online Access:http://www.frontiersinzoology.com/content/6/1/30
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spelling ftbiomed:oai:biomedcentral.com:1742-9994-6-30 2023-05-15T15:06:03+02:00 Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada Zhou, Xin Adamowicz, Sarah J Jacobus, Luke M DeWalt, R Edward Hebert, Paul DN 2009-12-10 http://www.frontiersinzoology.com/content/6/1/30 en eng BioMed Central Ltd. http://www.frontiersinzoology.com/content/6/1/30 Copyright 2009 Zhou et al; licensee BioMed Central Ltd. Research 2009 ftbiomed 2010-01-03T01:09:50Z Abstract Background This study reports progress in assembling a DNA barcode reference library for Ephemeroptera, Plecoptera, and Trichoptera ("EPTs") from a Canadian subarctic site, which is the focus of a comprehensive biodiversity inventory using DNA barcoding. These three groups of aquatic insects exhibit a moderate level of species diversity, making them ideal for testing the feasibility of DNA barcoding for routine biotic surveys. We explore the correlation between the morphological species delineations, DNA barcode-based haplotype clusters delimited by a sequence threshold (2%), and a threshold-free approach to biodiversity quantification--phylogenetic diversity. Results A DNA barcode reference library is built for 112 EPT species for the focal region, consisting of 2277 COI sequences. Close correspondence was found between EPT morphospecies and haplotype clusters as designated using a standard threshold value. Similarly, the shapes of taxon accumulation curves based upon haplotype clusters were very similar to those generated using phylogenetic diversity accumulation curves, but were much more computationally efficient. Conclusion The results of this study will facilitate other lines of research on northern EPTs and also bode well for rapidly conducting initial biodiversity assessments in unknown EPT faunas. Other/Unknown Material Arctic Churchill Subarctic BioMed Central Arctic Canada
institution Open Polar
collection BioMed Central
op_collection_id ftbiomed
language English
description Abstract Background This study reports progress in assembling a DNA barcode reference library for Ephemeroptera, Plecoptera, and Trichoptera ("EPTs") from a Canadian subarctic site, which is the focus of a comprehensive biodiversity inventory using DNA barcoding. These three groups of aquatic insects exhibit a moderate level of species diversity, making them ideal for testing the feasibility of DNA barcoding for routine biotic surveys. We explore the correlation between the morphological species delineations, DNA barcode-based haplotype clusters delimited by a sequence threshold (2%), and a threshold-free approach to biodiversity quantification--phylogenetic diversity. Results A DNA barcode reference library is built for 112 EPT species for the focal region, consisting of 2277 COI sequences. Close correspondence was found between EPT morphospecies and haplotype clusters as designated using a standard threshold value. Similarly, the shapes of taxon accumulation curves based upon haplotype clusters were very similar to those generated using phylogenetic diversity accumulation curves, but were much more computationally efficient. Conclusion The results of this study will facilitate other lines of research on northern EPTs and also bode well for rapidly conducting initial biodiversity assessments in unknown EPT faunas.
format Other/Unknown Material
author Zhou, Xin
Adamowicz, Sarah J
Jacobus, Luke M
DeWalt, R Edward
Hebert, Paul DN
spellingShingle Zhou, Xin
Adamowicz, Sarah J
Jacobus, Luke M
DeWalt, R Edward
Hebert, Paul DN
Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada
author_facet Zhou, Xin
Adamowicz, Sarah J
Jacobus, Luke M
DeWalt, R Edward
Hebert, Paul DN
author_sort Zhou, Xin
title Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada
title_short Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada
title_full Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada
title_fullStr Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada
title_full_unstemmed Towards a comprehensive barcode library for arctic life - Ephemeroptera, Plecoptera, and Trichoptera of Churchill, Manitoba, Canada
title_sort towards a comprehensive barcode library for arctic life - ephemeroptera, plecoptera, and trichoptera of churchill, manitoba, canada
publisher BioMed Central Ltd.
publishDate 2009
url http://www.frontiersinzoology.com/content/6/1/30
geographic Arctic
Canada
geographic_facet Arctic
Canada
genre Arctic
Churchill
Subarctic
genre_facet Arctic
Churchill
Subarctic
op_relation http://www.frontiersinzoology.com/content/6/1/30
op_rights Copyright 2009 Zhou et al; licensee BioMed Central Ltd.
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