Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures
Abstract Background The bacteriology during storage of the North-Atlantic cod has been investigated for the past decades using conventional cultivation strategies which have generated large amount of information. This paper presents a study where both conventional cultivation and cultivation indepen...
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ftbiomed:oai:biomedcentral.com:1471-2180-9-250 2023-05-15T15:27:18+02:00 Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures Reynisson, Eyjólfur Lauzon, Hélène L Magnússon, Hannes Jónsdóttir, Rósa Ólafsdóttir, Guðrún Marteinsson, Viggó Hreggviðsson, Guðmundur 2009-12-04 http://www.biomedcentral.com/1471-2180/9/250 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-2180/9/250 Copyright 2009 Reynisson et al; licensee BioMed Central Ltd. Research article 2009 ftbiomed 2009-12-27T00:24:36Z Abstract Background The bacteriology during storage of the North-Atlantic cod has been investigated for the past decades using conventional cultivation strategies which have generated large amount of information. This paper presents a study where both conventional cultivation and cultivation independent approaches were used to investigate the bacterial succession during storage of cod loins at chilled and superchilled temperatures. Results Unbrined (0.4% NaCl) and brined (2.5% NaCl) cod loins were stored at chilled (0°C) and superchilled (-2 and -3.6°C) temperatures in air or modified atmosphere (MA, % CO 2 /O 2 /N 2 : 49.0 ± 0.6/7.4 ± 0.2/43.7 ± 0.4). Discrepancy was observed between cultivation enumeration and culture independent methods where the former showed a general dominance of Pseudomonas spp. (up to 59%) while the latter showed a dominance of Photobacterium phosphoreum (up to 100%). Gas chromatography-mass spectrophotometry (GC-MC) showed that trimethylamine was the most abundant volatile in mid- and late storage periods. Terminal restriction polymorphism (t-RFLP) analysis showed that the relative abundance of P. phosphoreum increased with storage time. Conclusion The present study shows the bacteriological developments on lightly salted or non-salted cod loins during storage at superchilled temperatures. It furthermore confirms the importance of P. phosphoreum as a spoilage organism during storage of cod loins at low temperatures using molecular techniques. The methods used compensate each other, giving more detailed data on bacterial population developments during spoilage. Article in Journal/Newspaper atlantic cod Gadus morhua North Atlantic BioMed Central |
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BioMed Central |
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ftbiomed |
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English |
description |
Abstract Background The bacteriology during storage of the North-Atlantic cod has been investigated for the past decades using conventional cultivation strategies which have generated large amount of information. This paper presents a study where both conventional cultivation and cultivation independent approaches were used to investigate the bacterial succession during storage of cod loins at chilled and superchilled temperatures. Results Unbrined (0.4% NaCl) and brined (2.5% NaCl) cod loins were stored at chilled (0°C) and superchilled (-2 and -3.6°C) temperatures in air or modified atmosphere (MA, % CO 2 /O 2 /N 2 : 49.0 ± 0.6/7.4 ± 0.2/43.7 ± 0.4). Discrepancy was observed between cultivation enumeration and culture independent methods where the former showed a general dominance of Pseudomonas spp. (up to 59%) while the latter showed a dominance of Photobacterium phosphoreum (up to 100%). Gas chromatography-mass spectrophotometry (GC-MC) showed that trimethylamine was the most abundant volatile in mid- and late storage periods. Terminal restriction polymorphism (t-RFLP) analysis showed that the relative abundance of P. phosphoreum increased with storage time. Conclusion The present study shows the bacteriological developments on lightly salted or non-salted cod loins during storage at superchilled temperatures. It furthermore confirms the importance of P. phosphoreum as a spoilage organism during storage of cod loins at low temperatures using molecular techniques. The methods used compensate each other, giving more detailed data on bacterial population developments during spoilage. |
format |
Article in Journal/Newspaper |
author |
Reynisson, Eyjólfur Lauzon, Hélène L Magnússon, Hannes Jónsdóttir, Rósa Ólafsdóttir, Guðrún Marteinsson, Viggó Hreggviðsson, Guðmundur |
spellingShingle |
Reynisson, Eyjólfur Lauzon, Hélène L Magnússon, Hannes Jónsdóttir, Rósa Ólafsdóttir, Guðrún Marteinsson, Viggó Hreggviðsson, Guðmundur Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures |
author_facet |
Reynisson, Eyjólfur Lauzon, Hélène L Magnússon, Hannes Jónsdóttir, Rósa Ólafsdóttir, Guðrún Marteinsson, Viggó Hreggviðsson, Guðmundur |
author_sort |
Reynisson, Eyjólfur |
title |
Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures |
title_short |
Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures |
title_full |
Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures |
title_fullStr |
Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures |
title_full_unstemmed |
Bacterial composition and succession during storage of North-Atlantic cod (Gadus morhua)at superchilled temperatures |
title_sort |
bacterial composition and succession during storage of north-atlantic cod (gadus morhua)at superchilled temperatures |
publisher |
BioMed Central Ltd. |
publishDate |
2009 |
url |
http://www.biomedcentral.com/1471-2180/9/250 |
genre |
atlantic cod Gadus morhua North Atlantic |
genre_facet |
atlantic cod Gadus morhua North Atlantic |
op_relation |
http://www.biomedcentral.com/1471-2180/9/250 |
op_rights |
Copyright 2009 Reynisson et al; licensee BioMed Central Ltd. |
_version_ |
1766357749931704320 |