Disturbance induced decoupling between host genetics and composition of the associated microbiome
Abstract Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental cond...
Main Authors: | , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BioMed Central Ltd.
2013
|
Subjects: | |
Online Access: | http://www.biomedcentral.com/1471-2180/13/252 |
id |
ftbiomed:oai:biomedcentral.com:1471-2180-13-252 |
---|---|
record_format |
openpolar |
spelling |
ftbiomed:oai:biomedcentral.com:1471-2180-13-252 2023-05-15T15:58:10+02:00 Disturbance induced decoupling between host genetics and composition of the associated microbiome Wegner, Karl Volkenborn, Nils Peter, Hannes Eiler, Alexander 2013-11-09 http://www.biomedcentral.com/1471-2180/13/252 en eng BioMed Central Ltd. http://www.biomedcentral.com/1471-2180/13/252 Copyright 2013 Wegner et al.; licensee BioMed Central Ltd. Microbiota Population structure Stress Pathogen Biological invasion Pacific oyster Crassostrea gigas Vibrio Mycoplasma Research article 2013 ftbiomed 2013-11-24T01:22:43Z Abstract Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results While the gill associated microbial communities in oysters were dominated by few abundant taxa (i.e. Sphingomonas, Mycoplasma ) the distribution of rare bacterial groups correlated to relatedness between the hosts under ambient conditions. Exposing the host to disturbance broke apart this relationship by removing rare phylotypes thereby reducing overall microbial diversity. Shifts in the microbiome composition in response to stress did not result in a net increase in genera known to contain potentially pathogenic strains. Conclusion The decrease in microbial diversity and the disassociation between population genetic structure of the hosts and their associated microbiome suggest that disturbance (i.e. stress) may play a significant role for the assembly of the natural microbiome. Such community shifts may in turn also feed back on the course of disease and the occurrence of mass mortality events in oyster populations. Article in Journal/Newspaper Crassostrea gigas Pacific oyster BioMed Central Pacific |
institution |
Open Polar |
collection |
BioMed Central |
op_collection_id |
ftbiomed |
language |
English |
topic |
Microbiota Population structure Stress Pathogen Biological invasion Pacific oyster Crassostrea gigas Vibrio Mycoplasma |
spellingShingle |
Microbiota Population structure Stress Pathogen Biological invasion Pacific oyster Crassostrea gigas Vibrio Mycoplasma Wegner, Karl Volkenborn, Nils Peter, Hannes Eiler, Alexander Disturbance induced decoupling between host genetics and composition of the associated microbiome |
topic_facet |
Microbiota Population structure Stress Pathogen Biological invasion Pacific oyster Crassostrea gigas Vibrio Mycoplasma |
description |
Abstract Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results While the gill associated microbial communities in oysters were dominated by few abundant taxa (i.e. Sphingomonas, Mycoplasma ) the distribution of rare bacterial groups correlated to relatedness between the hosts under ambient conditions. Exposing the host to disturbance broke apart this relationship by removing rare phylotypes thereby reducing overall microbial diversity. Shifts in the microbiome composition in response to stress did not result in a net increase in genera known to contain potentially pathogenic strains. Conclusion The decrease in microbial diversity and the disassociation between population genetic structure of the hosts and their associated microbiome suggest that disturbance (i.e. stress) may play a significant role for the assembly of the natural microbiome. Such community shifts may in turn also feed back on the course of disease and the occurrence of mass mortality events in oyster populations. |
format |
Article in Journal/Newspaper |
author |
Wegner, Karl Volkenborn, Nils Peter, Hannes Eiler, Alexander |
author_facet |
Wegner, Karl Volkenborn, Nils Peter, Hannes Eiler, Alexander |
author_sort |
Wegner, Karl |
title |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_short |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_full |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_fullStr |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_full_unstemmed |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_sort |
disturbance induced decoupling between host genetics and composition of the associated microbiome |
publisher |
BioMed Central Ltd. |
publishDate |
2013 |
url |
http://www.biomedcentral.com/1471-2180/13/252 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://www.biomedcentral.com/1471-2180/13/252 |
op_rights |
Copyright 2013 Wegner et al.; licensee BioMed Central Ltd. |
_version_ |
1766393904536485888 |